Dr. Manoj Kumar Singh
Senior Scientist
Animal Biotechnology Centre, ICAR-NDRI, Karnal
Email: manoj.singh@icar.gov.in
Current Research Area
Embryo Biotechnology, Somatic Cell Cloning, Stem Cell Biology, Transgenesis, Ovum pick-up.
Qualification:
| Degree | Name of the College/Institute and University | Passing Year |
| Ph.D.
(Animal Biotechnology) |
ICAR-N.D.R.I., Karnal | 2009 |
| M.V.Sc. & A.H.
(Veterinary Biotechnology) |
Biotechnology Centre, J.N.K.V.V., Jabalpur | 2004 |
| B.V.Sc. & A.H. | Bombay Veterinary College, Mumbai/M.A.F.S.U., Nagpur | 2002 |
Research:
Research Projects handled/being handled:
| Title of the project | Level of association (PI/Co-PI) | Period | Sponsoring organization
|
Project Cost in Lakhs | ||
| From
(DD/MM/YYYY) |
To
(DD/MM/YYYY) |
|||||
| ON-GOING | ||||||
| 1. | Conservation of Indigenous Pig of Assam through Handmade Cloning technique | PI
(Collaborative Institute) |
02.01.2017 | 02.07.2020 | DBT, New Delhi | 40.59 Lakh
(NDRI) |
| 2. | Global transcriptome and miRNA analysis for deciphering reasons for low cloning efficiency in buffalo | Co-PI | 22.03.2018 | 21.03.2021 | DST, New Delhi
|
46.78 Lakh |
| 3. | Production of multiple copies of elite buffalo bulls using animal cloning technology | Co-PI | 01.04.2018 | 31.03.2022 | NASF, New Delhi | 264.3 Lakh |
| COMPLETED | ||||||
| 4. | Synthetic Endometrium: A novel model to study early embryonic development and uterine health in ruminants | Co-PI | 01.04.2017 | 31.03.2020 | NASF (ICAR) | 77.48 Lakhs
(NDRI) |
| 5. | Generation of induced pluripotent stem (iPS) cell like cells from buffalo fibroblasts | PI | 01.04.2014 | 31.03.2017 | N.D.R.I., Karnal (Institute Project) | 27.00 Lakh |
| 6. | Development of parthenogenetic goat from embryonic stem cells | PI
(Collaborative Centre) |
01.04.2013 | 31.03.2017 | NFBSFARA (ICAR) | 90.84 Lakhs |
| 7. | Semen sexing in cattle
|
Co-PI | June 2015 | 31.03.2017 | ICAR, New Delhi | |
| 8. | Expression of apoptosis-related genes in buffalo embryos produced in vitro under different oxygen concentrations. | PI | January, 2010 | Dec, 2012 | N.D.R.I., Karnal
(Institute Project) |
49.56 Lakh |
| 9. | Establishment of facilities for research and training in stem cell technology in pigs. | Co-PI
PI from March 30, 2016 |
April, 2012 | October, 2016 | DBT, New Delhi | 37.38 Lakhs |
| 10. | Buffalo transgenesis for expression of desired proteins. | Co-PI
PI from March 30, 2016 |
June, 2014 | June, 2016 | DBT, New Delhi | 59.078 Lakhs |
| 11. | Development of transgenic goat for production of human lactoferrin. | (Co-PI)
PI from March 30, 2016 |
June, 2012 | May, 2016 | NFBSFARA (ICAR) | 253.68 Lakhs |
| 12. | Characterization and differentiation of embryonic and spermatogonial stem cells in cattle and buffaloes. | Co-PI | Sep. 2008 Joined from July, 2009 | June, 2014 | N.A.I.P., New Delhi | 398.51 Lakhs |
| 13. | Development of transgenes for in vitro expression in buffalo mammary gland epithelial cells. | Co-PI | Sep. 2011 | Sep. 2013 | DBT, New Delhi | 43.08 Lakhs |
| 14. | Improvement in fertilizability of cryopreserved buffalo bull semen by minimizing cryocapacitation and apoptosis like changes. | Co-PI | March, 2011 | Feb, 2013 | DBT, New Delhi | 17 Lakhs
|
| 15. | Molecular cloning and expression of buffalo leukemia inhibitory factor (LIF) in eukaryotic system. | Co-PI | January, 2010 | Dec, 2012 | N.D.R.I., Karnal
(Institute Project) |
Rs9 Lakh |
| 16. | Embryo production by Ovum Pick-Up from live cattle. | Co-PI | Sep. 2008 Joined from July, 2009 | August, 2011 | DBT, New Delhi | Rs 58.88 Lakh |
- Teaching:
| S. No. | Course code | Course nomenclature | Credit hours |
| 1. | BT – 613 | Immunology Applied to Biotechnology | (2+1) |
| 2. | BT – 614 | Reproductive Biotechnology | (2+1) |
| 3. | BT – 713 | Advances in Reproductive Biotechnology | (3+0) |
| 4. | BT – 722 | Advances in Animal Cell Culture | (3+0) |
| 5. | LP-411 | Anatomy of Farm Animals | (2+1) |
| 6. | GS – 635 | Disaster Management | (1+0) |
- Publications: 46
| S. No. | Title | Authors | Year/journal |
| Articles in Foreign Referred Journals (39) | |||
| 1. | Propagation of goat putative spermatogonial stem cells under growth factors defined serum-free culture conditions. | Sharma A, Shah SM, Tiwari M, Roshan M, SinghMK, Singla SK, Palta P, Manik RS and Chauhan MS. | 2020 Cytotechnology, 72:489-497. |
| 2. | Successful transplantation of transfected enriched buffalo (Bubalus bubalis) spermatogonial stem cells to homologous recipients. | A. Sharma, A. Kumaresan, P. Mehta, N. Nala, M.K. Singh, P. Palta, S.K. Singla, R.S. Manik and M.S. Chauhan. | 2020
Theriogenology, 142: 441-449. |
| 3. | Media switching at different time periods affects the reprogramming efficiency of buffalo fetal fibroblasts. | N. Rawat, M.K. Singh, T. Sharma P. Vats, D. Nagoorvali, P. Paltaa, M.S. Chauhan, and R.S. Manik.
|
2019
Animal Biotechnology, DOI: 10.1080/10495398.2019.1671435 |
| 4. | Selection of Reference miRNAs for Relative Quantification in Buffalo (Bubalus bubalis) Blastocysts Produced by Hand-Made Cloning and In Vitro Fertilization. | S.V. Lagah, T.J. Sood, P. Palta, M. Mukesh, M.S. Chauhan, R.S. Manik, M.K. Singh and S.K. Singla.
|
2019
Cellular Reprogramming, 21(4): 200-209. |
| 5. | Calcium ionophore enhanced developmental competence and apoptotic dynamics of goat parthenogenetic embryos produced in vitro. | Dua D., Nagoorvali D., Chauhan M.S., Palta P., Mathur P., Singh M.K. | 2019
In Vitro Cell Dev Biol Anim. 55(3):159-168 |
| 6. | Comparative analysis of buffalo (Bubalus bubalis) non-transgenic and transgenic embryos containing human insulin gene, produced by SCNT. | P. Mehta, R. Kaushik, K.P. Singh, A. Sharma, M.K. Singh, M.S. Chauhan, P. Palta, S.K. Singla, R.S. Manik. | 2019
Theriogenology 135 25e32 |
| 7. | Supplementation of glial cell line-derived neurotrophic factor, fibroblast growth factor 2, and epidermal growth factor promotes self-renewal of putative buffalo (Bubalus bubalis) spermatogonial stem cells by upregulating the expression of mir-20b, mir-21, and mir-106a. | A. Sharma, S.V. Lagah, D. Nagoorvali, B.S.B. Kumar, M.K.Singh, S.K. Singla, R.S.Manik, P. Palta,and M.S. Chauhan. | 2019
Cell Reprogram. 2019Feb;21(1):11-17. |
| 8. | Optimization of serum-free culture conditions for propagation of putative buffalo (Bubalus bubalis) spermatogonial stem cells. | A. Sharma, S.M. Shah, N. Saini,P. Mehta, B.S.B. Kumar, D. Dua, M.K. Singh, S.K.Singla, P. Palta, R.S. Manik and M.S. Chauhan. | 2019
Cell Reprogram. 2019 21(1):1-10. |
| 9. | Treatment of buffalo (Bubalus bubalis) SCNT embryos with microRNA-21 mimic improves their quality and alters gene expression but does not affect their developmental competence. | Rashmi, S. Sah, S. Shyam, M.K. Singh, P. Palta. | 2019
Theriogenology 126, 8-16 |
| 10. | Epigenetic alteration of donor cells with histone deacetylase inhibitor m- carboxycinnamic acid bishydroxymide (CBHA) improves the in vitro developmental competence of buffalo (Bubalus bubalis) cloned embryos. | Agarwal H, Selokar NL, Saini M, Singh MK, Chauhan MS, Palta P, Singla SK, Manik RS. | 2018
Cell Reprogram 20(1):76-88. |
| 11. | m-carboxycinnamic acid bishydroxamide improves developmental competence, reduces apoptosis and alters epigenetic status and gene expression pattern in cloned buffalo (Bubalus bubalis) embryos. | Agarwal, H., Selokar, N.L., Saini, M., Singh, M.K., Chauhan, M.S., Palta, P., Singla, S.K. and Manik, R.S. | 2018 Reproduction in Domestic Animals. Doi:10.1111/rda.13198. |
| 12. | Establishment, growth, proliferation and gene expression of buffalo (Bubalus bubalis) transgenic fetal fibroblasts containing human insulin gene, and production of embryos by hand-made cloning using these cells. | Mehta, P., Kaushik, R., Singh, K.P., Sharma, A., Singh, M.K., Chauhan, M.S., Palta, P., Singla, S.K. ans Manik R.S. (2018). | 2018
Cellular Reprogramming 20(2): 135-143. |
| 13. | Testicular cell-conditioned medium supports embryonic stem cell differentiation toward germ lineage and to spermatocyte- and oocyte-like cells. | Shah SM, Saini N, Singh MK, Manik RS, Singla SK, Palta P and Chauhan MS. 2016. | 2016
Theriogenology, 86 (3): 715-729. |
| 14. | Effect of sex of embryo on developmental competence, epigenetic status, and gene expression in buffalo (Bubalus bubalis) embryos produced by hand-made cloning. | Sandhu A, Mohapatra SK, Agrawal H, Singh MK, Palta P, Singla SK, Chauhan MS, Manik RS. | 2016
Cell Reprogram, 18(5):356-365. |
| 15. | Supplementation of tauroursodeoxycholic acid during IVC did not enhance in vitro development and quality of buffalo IVF embryos but combated endoplasmic reticulum stress. | A. Sharma, H. Agrawal, N. Mullani, A. Sandhu,M.K. Singh, M.S. Chauhan, S.K.Singla, P. Palta, R.S. Manik. | 2015
Theriogenology 84 (2015) 200-207 |
| 16. | Caspase-9 inhibitor Z-LEHD-FMK enhances the yield of in vitro produced buffalo (Bubalus bubalis) pre-implantation embryos and alters cellular stress response. | Mullani N, Singh MK, Sharma A, Rameshbabu K, Manik RS, Palta P, Singla SK and Chauhan MS. | 2015
Research in Veterinary Science. 104:4-9. |
| 17. | Developmental competence of different quality bovine oocytes retrieved through ovum pick-up following in vitro maturation and fertilization. | Saini N, Singh MK, Shah SM, Singh KP, Kaushik R, Manik RS, Singla SK, Palta P and Chauhan MS. | 2015
Animal. 9(12):1979-1985. |
| 18. | Molecular characterization and expression of buffalo (Bubalus bubalis) DEAD-box family VASA gene and mRNA transcript variants isolated from testis tissue. | Kaushik R, Singh KP, Bahuguna V, Rameshbabu K, Singh MK, Manik RS, Palta P, Singla SK and Chauhan MS. 2015. | 2015
Gene. 572(1):17-26. |
| 19. | Cumulus cell-conditioned medium supports embryonic stem cell differentiation to germ cell-like cells. | Shah SM, Saini N, Ashraf S, Singh MK, Manik RS, Singla SK, Palta P and Chauhan MS. 2015. | 2015
Reproduction Fertility and Development. 29: 679-693. |
| 20. | Comparative expression analysis of gametogenesis-associated genes in foetal and adult bubaline (Bubalus bubalis) ovaries and testes | Shah SM, Saini N, Ashraf S, Zandi M, Singh MK, Manik RS and Chauhan MS. 2015. | 2015
Reproduction in Domestic Animals. 50(3), 365-377. |
| 21. | Optimization of Buffalo (Bubalus bubalis) Embryonic Stem Cell Culture System. | Zandi M, Muzaffar M, Shah SM, Singh MK, Palta P, Kumar Singla S, Manik R and Chauhan MS. | 2015
Cell J. 17(2):264-73. |
| 22. | Activation and inhibition of the Wnt3A signaling pathway in buffalo (Bubalus bubalis) embryonic stem cells: effects of WNT3A, Bio and Dkk1. | Zandi M, Shah SM, Muzaffar M, Singh MK, Palta P, Singla SK, Manik RS and Chauhan MS. | 2015
International Journal of Fertility and Sterility. 9(3): 361-70. |
| 23. | Developmental competence and expression pattern of bubaline (Bubalus bubalis) oocytes subjected to elevated temperatures during meiotic maturation in vitro. | Ashraf S, Shah SM, Saini N, Dhanda S, Kumar A, Goud TS, Singh MK, Chauhan MS and Upadhyay RC. | 2014
Journal of assisted reproduction and genetics 31 (10), 1349-1360. |
| 24. | Quantitative expression of pluripotency-related genes in parthenogenetically produced buffalo (Bubalus bubalis) embryos and in putative embryonic stem cells derived from them. | Singh, K.P., Kaushik, R., Mohapatra, S.K., Garg, V., Rameshbabu, K., Singh, M.K., Palta, P., Manik, R.S., Singla, S.K. and Chauhan, M.S. 2014. | 2014
Gene Expression Patterns 16(1), 23-30. |
| 25. | Construction of a recombinant human insulin expression vector for mammary gland-specific expression in buffalo (Bubalus bubalis) mammary epithelial cell line. | Kaushik, R., Singh, K.P., Kumari, A., Rameshbabu, K., Singh, M.K., Manik, R.S., Palta, P., Singla, S.K. and Chauhan, M.S. 2014. | 2014
Molecular Biology Reports 41(9), 5891-5902. |
| 26. | Buffalo (Bubalus bubalis) ES cell-like cells are capable of in vitro skeletal myogenic differentiation. | M.K. Singh, K.P. Singh, D. Kumar, R.A. Shah, T. Anand, M.S. Chauhan, R.S. Manik, S.K. Singla and P. Palta. 2013. | Reprod. Dom. Anim., 48(2):284-91. |
| 27. | WNT3A signaling pathway in buffalo (Bubalus bubalis) embryonic stem cells. | Zandi M, Muzaffar M, Shah SM, Kaushik R, Singh MK, Palta P, Singla SK, Manik RS and Chauhan MS. 2013. | 2013
Reproduction, Fertility and Development: 26: 551-561. |
| 28. | Effect of physiologically relevant heat shock on development, apoptosis and expression of some genes in buffalo (Bubalus bubalis) embryos produced in vitro. | A. Yadav, K.P. Singh, M.K. Singh, N. Saini, P. Palta, R.S. Manik, S.K. Singla, R.C. Upadhyay and M.S. Chauhan. 2013. | 2013
Reproduction in Domestic Animals, 48(5): 858-865. |
| 29. | Isolation, characterization, and EGFP expression in the buffalo (Bubalus bubalis) mammary gland epithelial cell line. | Kaushik R, Singh KP, Kumari A, Singh MK, Manik RS, Palta P, Singla SK and Chauhan MS. | 2013
In Vitro Cellular & Dev Biol – Animal 49(1): 1-7. |
| 30. | Effects of GDNF, FGF2 and EGF on proliferation and expression of some important genes in buffalo (Bubalus bubalis) spermatogonial cells. | P.H. Kadam, S. Kala, H. Agrawal, K.P. Singh, M.K. Singh, M.S. Chauhan, P. Palta, S.K. Singla and R.S. Manik. | 2012
Reproduction, Fertility and Development. 25: 1149-1157. |
| 31. | Equivalency of buffalo (Bubalus bubalis) embryonic stem cells derived from fertilized, parthenogenetic and handmade cloned embryos. | M. Muzaffar, N.L. Selokar, K.P. Singh, M. Zandi, M.K. Singh, R.A. Shah, M.S. Chauhan, S.K. Singla, P. Palta and R.S. Manik. | 2012
Cellular Reprogramming, 14(3):267-79. |
| 32. | In vitro culture and morphological characterization of prepubertal buffalo (Bubalus bubalis) putative spermatogonial stem cell. | Kala S, Kaushik R, Singh KP, Kadam PH, Singh MK, Manik RS, Singla SK, Palta P, Chauhan MS. | 2012
J. Assist. Reprod. Genet. 29(12): 1335-1342. |
| 33. | Expression pattern of pluripotent markers in different embryonic developmental stages of buffalo (Bubalus bubalis) embryos and putative embryonic stem cells generated by parthenogenetic activation. | Singh KP, Kaushik R, Garg V, Sharma R, George A, Singh MK, Manik RS, Palta P, Singla SK, Chauhan MS. | 2012
Cell. Reprogram. 14(6): 530-538. |
| 34. | Oxygen concentration and cysteamine supplementation during in vitro production of buffalo (Bubalus bubalis) embryos affect mRNA expression of BCL-2, BCL-XL, MCL-1, BAX and BID. | Elamaran G, Singh KP, Singh MK, Singla SK, Chauhan MS, Manik RS, Palta P. | 2012
Reprod. Domest. Anim. 47(6): 1027-1036. |
| 35. | Derivation of buffalo embryonic stem-like cells from in vitro-produced blastocysts on homologous and heterologous feeder cells. | Kumar, D., Anand, T., Singh, K.P., Singh, M.K., Shah, R.A., Chauhan, M.S. Palta, P., Singla, S.K., and Manik, R.S. | 2011
J Assist Reprod Genet., 28: 697-688. |
| 36. | Buffalo (Bubalus bubalis) embryonic stem cell-like cells and preimplantation embryos exhibit comparable expression of pluripotency-related antigens. | T. Anand, D. Kumar, M.K. Singh, R.A Shah, M.S. Chauhan, R.S. Manik, S.K. Singla and P. Palta. | 2011
Reprod. Dom. Anim. 6: 50-58. |
| 37. | Effect of sodium nitroprusside, a nitric oxide donor, and aminoguanidine, a nitric oxide synthase inhibitor, on in vitro development of buffalo (Bubalus bubalis) embryos. | S. Saugandhika, D. Kumar, M.K. Singh, R. Shah, T. Anand, M.S.Chauhan, R.S. Manik, S.K. Singla, P. Palta. | 2010
Reprod. Dom. Anim., 45 (5): 931-933. |
| 38. | Pregnancies established from handmade cloned blastocysts reconstructed using skin fibroblasts in buffalo (Bubalus bubalis). | R.A. Shah, A. George, M.K. Singh, D. Kumar, T. Anand, M.S. Chauhan, R.S. Manik, P. Palta and S.K. Singla. | 2009 Theriogenology. 71(8):1215-9. |
| 39. | Hand-Made Cloned Buffalo (Bubalus bubalis) Embryos: Comparison of Different Media and Culture Systems. | R.A. Shah, A. George, M.K. Singh, D. Kumar, T. Anand, M.S. Chauhan, R.S. Manik, P. Palta and S.K. Singla. | 2008
Cloning and Stem Cells. 10 (4):435-42. (Now Cellular Reprogramming) |
| Articles in Indian Referred Journals (07) | |||
| 40. | Comparative study of three different media to improve semen processing and flow cytometric analysis in bovine. | Nala N., Kumar V., Singh M.K., Manik R.S. and Singla S.K.
|
2017
Journal of Animal Research, 7(3): 537-541. |
| 41. | Derivation, enrichment and characterization of goat (Capra hircus) spermatogonial stem cells from pre-pubertal testes. | Sharma A, Shah SM, Saini N, Kaushik R, Singh MK, Manik RS, Singla SK, Palta P and Chauhan MS. | 2016
Indian J. Anim. Res., 50(5): 662-667. |
| 42. | ‘Holi’: India’s first cattle calf produced through ovum pick-up – IVF technology- from an aged sahiwal cattle. | Saini N, Singh MK, Singh KP, Manik RS, Singla SK, Palta P and Chauhan MS. | 2015
Indian J. Animal Sci. 85(3), 244-246. |
| 43. | Expression of apoptosis related genes in buffalo embryos produced through in vitro fertilization and parthenogenetic activation. | Singh, MK, Saw S, Singh KP, Saini N, Kaushik R, Manik RS, Palta P, Singla SK and Chauhan MS. | 2014
Indian Journal of Animal Sciences84 (2): 146–151. |
| 44. | Characterization of the coding region of basic fibroblast growth factor (FGF2) c-DNA (open reading frame) in buffalo cumulus cells. | Kumar S, Singh MK, Kumar V, Palta P, Singla SK, Manik RS and Chauhan MS. (2013). | Indian Journal of Animal Sciences 83(7):744-746. |
| 45. | Expression patterns of OCT-4 and NANOG genes in buffalo (Bubalus bubalis) embryos produced by in vitro fertilization or parthenogenetic activation. | Kumar D, Anand T, Shah RA, Singh MK, Chauhan MS and Manik RS. | 2012
Ind. J. Anim. Sci. 82(8): 834-837. |
| 46. | Generation of buffalo embryonic stem cell-like cells from in vitro produced, day 8 hatched and day 9 expanded blastocysts | Kumar D, Anand T, Singh KP, Shah RA, Singh MK, Chauhan MS, Palta P, Singla SK and Manik RS. | 2012
Ind. J. Anim. Sci. 82(8): 838-843. |
Present Lab Members: Dr. M.K. Singh & Dr. P. Palta
Students currently enrolled
| Sr. No. | Name of student | Class | Dissertation topic: |
| 1. | Mr. Gaurav Tripathi
(15-P-BT-9) |
Ph.D. Scholar | Effect of miRNA- 29b and miRNA- 449b on buffalo transgenic embryos, containing human insulin gene, produced by handmade cloning. |
| 2. | Ms. Tanya Gupta
(16-P-BT-06) |
Ph.D. Scholar | Global DNA methylation profile of spermatozoa of cloned buffalo bulls and their respective donor bulls. |
| 3. | Ms. Kumari Rinka
(16-P-BT-07) |
Ph.D. Scholar | Effect of trichostatin A and 5-aza-2’-deoxycytidine on the developmental competence and transcriptome profile of buffalo embryos produced by Hand-made cloning” |
| 4. | Ms. Sonal Gupta
(17-P-BT-13) |
Ph.D. Scholar | Effect of oxamflatin and ascorbic acid on the developmental competence and transcriptome profile of buffalo embryos produced by Hand- made cloning (Proposed). |
| 5. | Mr. Atul Kaushal
(18-P-BT-11) |
Ph.D. Scholar | Course work |
| 6. | Mr. Prem Kumar
(19-P-BT-13) |
Ph.D. Scholar | Course work |
| 7. | Mr. Faiz Akram
(16-M-BT-06) |
M.Sc. Scholar | Course work |
| 8. | Ms. Smriti Gupta
(18-M-BT-09) |
M.Sc. Scholar | Course work |
| 9. | Ms. Kanika Gandhi
(16-M-BT-02) |
M.Sc. Scholar | Course work |
| 10. | Ms. Ritika
(19-M-BT-10) |
M.Sc. Scholar | Course work |
Technologies Developed (if any):
- Ovum pick-up-IVF technique standardised in indigenous cattle (India’s first cattle calf “Holi” produced)
- Hand-guided Cloning protocol in buffalo developed.
- Established the procedure of buffalo embryonic stem cell culture and their induced differentiation into myogenic cells.
- Establishment of spermatogonial stem cell line in buffalo and goat.
- Protocol established for transgenic embryo production in goat and buffalo.
