• Dr. Manoj Kumar Singh
    Senior Scientist

    Animal Biotechnology Centre, ICAR-NDRI, Karnal

    • Current Research Area
      Embryo Biotechnology, Somatic Cell Cloning, Stem Cell Biology, Transgenesis, Ovum pick-up.


    Degree Name of the College/Institute and University Passing Year


    (Animal Biotechnology)

    ICAR-N.D.R.I., Karnal 2009
    M.V.Sc. & A.H.


    (Veterinary Biotechnology)

    Biotechnology Centre, J.N.K.V.V., Jabalpur 2004
    B.V.Sc. & A.H. Bombay Veterinary College, Mumbai/M.A.F.S.U., Nagpur 2002


    Research Projects handled/being handled:


    Title of the project Level of association (PI/Co-PI) Period Sponsoring organization



    Project Cost in Lakhs






    1. Conservation of Indigenous Pig of Assam through Handmade Cloning technique PI


    (Collaborative Institute)

    02.01.2017 02.07.2020 DBT, New Delhi 40.59 Lakh



    2. Global transcriptome and miRNA analysis for deciphering reasons for low cloning efficiency in buffalo Co-PI 22.03.2018 21.03.2021 DST, New Delhi



    46.78 Lakh
    3. Production of multiple copies of elite buffalo bulls using animal cloning technology Co-PI 01.04.2018 31.03.2022 NASF, New Delhi 264.3 Lakh
    4. Synthetic Endometrium: A novel model to study early embryonic development and uterine health in ruminants Co-PI 01.04.2017 31.03.2020 NASF (ICAR) 77.48 Lakhs



    5. Generation of induced pluripotent stem (iPS) cell like cells from buffalo fibroblasts PI 01.04.2014 31.03.2017 N.D.R.I., Karnal (Institute Project) 27.00 Lakh
    6. Development of parthenogenetic goat from embryonic stem cells PI


    (Collaborative Centre)

    01.04.2013 31.03.2017 NFBSFARA (ICAR) 90.84 Lakhs
    7. Semen sexing in cattle



    Co-PI June 2015 31.03.2017 ICAR, New Delhi  
    8. Expression of apoptosis-related genes in buffalo embryos produced in vitro under different oxygen concentrations. PI January, 2010 Dec, 2012 N.D.R.I., Karnal


    (Institute Project)

    49.56 Lakh
    9. Establishment of facilities for research and training in stem cell technology in pigs. Co-PI


    PI from March 30, 2016

    April, 2012 October, 2016 DBT, New Delhi 37.38 Lakhs
    10. Buffalo transgenesis for expression of desired proteins. Co-PI


    PI from March 30, 2016

    June, 2014 June, 2016 DBT, New Delhi 59.078 Lakhs
    11. Development of transgenic goat for production of human lactoferrin. (Co-PI)


    PI from March 30, 2016

    June, 2012 May, 2016 NFBSFARA (ICAR) 253.68 Lakhs
    12. Characterization and differentiation of embryonic and spermatogonial stem cells in cattle and buffaloes. Co-PI Sep. 2008  Joined from July, 2009 June, 2014 N.A.I.P., New Delhi 398.51 Lakhs
    13. Development of transgenes for in vitro expression in buffalo mammary gland epithelial cells. Co-PI Sep. 2011 Sep. 2013 DBT, New Delhi 43.08 Lakhs
    14. Improvement in fertilizability of cryopreserved buffalo bull semen by minimizing cryocapacitation and apoptosis like changes. Co-PI  March, 2011 Feb, 2013 DBT, New Delhi 17 Lakhs



    15. Molecular cloning and expression of buffalo leukemia inhibitory factor (LIF) in eukaryotic system. Co-PI January, 2010 Dec, 2012 N.D.R.I., Karnal


    (Institute Project)

    Rs9 Lakh
    16. Embryo production by Ovum Pick-Up from live cattle. Co-PI Sep. 2008 Joined from July, 2009 August, 2011 DBT, New Delhi Rs 58.88 Lakh
    1. Teaching:
    S. No. Course code Course nomenclature Credit hours
    1. BT – 613 Immunology Applied to Biotechnology (2+1)
    2. BT – 614 Reproductive Biotechnology (2+1)
    3. BT – 713 Advances in Reproductive Biotechnology (3+0)
    4. BT – 722 Advances in Animal Cell Culture (3+0)
    5. LP-411 Anatomy of Farm Animals (2+1)
    6. GS – 635 Disaster Management (1+0)


    1. Publications: 46
    S. No. Title Authors Year/journal
    Articles in Foreign Referred Journals (39)
    1. Propagation of goat putative spermatogonial stem cells under growth factors defined serum-free culture conditions. Sharma A, Shah SM, Tiwari M, Roshan M, SinghMK, Singla SK, Palta P, Manik RS and Chauhan MS. 2020 Cytotechnology, 72:489-497.
    2. Successful transplantation of transfected enriched buffalo (Bubalus bubalis) spermatogonial stem cells to homologous recipients. A. Sharma, A. Kumaresan, P. Mehta, N. Nala, M.K. Singh, P. Palta, S.K. Singla, R.S. Manik and M.S. Chauhan. 2020


    Theriogenology, 142: 441-449.

    3. Media switching at different time periods affects the reprogramming efficiency of buffalo fetal fibroblasts. N. Rawat, M.K. Singh, T. Sharma P. Vats, D. Nagoorvali, P. Paltaa, M.S. Chauhan, and R.S. Manik.





    Animal Biotechnology, DOI: 10.1080/10495398.2019.1671435

    4. Selection of Reference miRNAs for Relative Quantification in Buffalo (Bubalus bubalis) Blastocysts Produced by Hand-Made Cloning and In Vitro Fertilization. S.V. Lagah, T.J. Sood, P. Palta, M. Mukesh, M.S. Chauhan, R.S. Manik, M.K. Singh and S.K. Singla.





    Cellular Reprogramming, 21(4): 200-209.

    5. Calcium ionophore enhanced developmental competence and apoptotic dynamics of goat parthenogenetic embryos produced in vitro. Dua D., Nagoorvali D., Chauhan M.S., Palta P., Mathur P., Singh M.K. 2019


    In Vitro Cell Dev Biol Anim.  55(3):159-168

    6. Comparative analysis of buffalo (Bubalus bubalis) non-transgenic and transgenic embryos containing human insulin gene, produced by SCNT. P. Mehta, R. Kaushik, K.P. Singh, A. Sharma, M.K. Singh, M.S. Chauhan, P. Palta, S.K. Singla, R.S. Manik. 2019


    Theriogenology 135 25e32

    7. Supplementation of glial cell line-derived neurotrophic factor, fibroblast growth factor 2, and epidermal growth factor promotes self-renewal of putative buffalo (Bubalus bubalis) spermatogonial stem cells by upregulating the expression of mir-20b, mir-21, and mir-106a. A. Sharma, S.V. Lagah, D. Nagoorvali, B.S.B. Kumar, M.K.Singh, S.K. Singla, R.S.Manik, P. Palta,and M.S. Chauhan. 2019


    Cell Reprogram. 2019Feb;21(1):11-17.

    8. Optimization of serum-free culture conditions for propagation of putative buffalo (Bubalus bubalis) spermatogonial stem cells. A. Sharma, S.M. Shah, N. Saini,P. Mehta, B.S.B. Kumar, D. Dua, M.K. Singh, S.K.Singla, P. Palta, R.S. Manik and M.S. Chauhan. 2019


    Cell Reprogram. 2019 21(1):1-10.

    9. Treatment of buffalo (Bubalus bubalis) SCNT embryos with microRNA-21 mimic improves their quality and alters gene expression but does not affect their developmental competence. Rashmi, S. Sah, S. Shyam, M.K. Singh, P. Palta. 2019


    Theriogenology 126, 8-16

    10. Epigenetic alteration of donor cells with histone deacetylase inhibitor m- carboxycinnamic acid bishydroxymide (CBHA) improves the in vitro developmental competence of buffalo (Bubalus bubalis) cloned embryos. Agarwal H, Selokar NL, Saini M, Singh MK, Chauhan MS, Palta P, Singla SK, Manik RS. 2018


    Cell Reprogram 20(1):76-88.

    11. m-carboxycinnamic acid bishydroxamide improves developmental competence, reduces apoptosis and alters epigenetic status and gene expression pattern in cloned buffalo (Bubalus bubalis) embryos. Agarwal, H., Selokar, N.L., Saini, M., Singh, M.K., Chauhan, M.S., Palta, P., Singla, S.K. and Manik, R.S. 2018 Reproduction in Domestic Animals. Doi:10.1111/rda.13198.
    12. Establishment, growth, proliferation and gene expression of buffalo (Bubalus bubalis) transgenic fetal fibroblasts containing human insulin gene, and production of embryos by hand-made cloning using these cells. Mehta, P., Kaushik, R., Singh, K.P., Sharma, A., Singh, M.K., Chauhan, M.S., Palta, P., Singla, S.K. ans Manik R.S. (2018). 2018


    Cellular Reprogramming 20(2): 135-143.

    13. Testicular cell-conditioned medium supports embryonic stem cell differentiation toward germ lineage and to spermatocyte- and oocyte-like cells. Shah SM, Saini N, Singh MK, Manik RS, Singla SK, Palta P and Chauhan MS. 2016. 2016


    Theriogenology, 86 (3): 715-729.

    14. Effect of sex of embryo on developmental competence, epigenetic status, and gene expression in buffalo (Bubalus bubalis) embryos produced by hand-made cloning. Sandhu A, Mohapatra SK, Agrawal H, Singh MK, Palta P, Singla SK, Chauhan MS, Manik RS. 2016


    Cell Reprogram, 18(5):356-365.

    15. Supplementation of tauroursodeoxycholic acid during IVC did not enhance in vitro development and quality of buffalo IVF embryos but combated endoplasmic reticulum stress. A. Sharma, H. Agrawal, N. Mullani, A. Sandhu,M.K. Singh, M.S. Chauhan, S.K.Singla, P. Palta, R.S. Manik. 2015


    Theriogenology 84 (2015) 200-207

    16. Caspase-9 inhibitor Z-LEHD-FMK enhances the yield of in vitro produced buffalo (Bubalus bubalis) pre-implantation embryos and alters cellular stress response. Mullani N, Singh MK, Sharma A, Rameshbabu K, Manik RS, Palta P, Singla SK and Chauhan MS. 2015


    Research in Veterinary Science. 104:4-9.

    17. Developmental competence of different quality bovine oocytes retrieved through ovum pick-up following in vitro maturation and fertilization. Saini N, Singh MK, Shah SM, Singh KP, Kaushik R, Manik RS, Singla SK, Palta P and Chauhan MS. 2015


    Animal. 9(12):1979-1985.

    18. Molecular characterization and expression of buffalo (Bubalus bubalis) DEAD-box family VASA gene and mRNA transcript variants isolated from testis tissue. Kaushik R, Singh KP, Bahuguna V, Rameshbabu K, Singh MK, Manik RS, Palta P, Singla SK and Chauhan MS. 2015. 2015


    Gene. 572(1):17-26.

    19. Cumulus cell-conditioned medium supports embryonic stem cell differentiation to germ cell-like cells. Shah SM, Saini N, Ashraf S, Singh MK, Manik RS, Singla SK, Palta P and Chauhan MS. 2015. 2015


    Reproduction Fertility and Development. 29: 679-693.

    20. Comparative expression analysis of gametogenesis-associated genes in foetal and adult bubaline (Bubalus bubalis) ovaries and testes Shah SM, Saini N, Ashraf S, Zandi M, Singh MK, Manik RS and Chauhan MS. 2015. 2015


    Reproduction in Domestic Animals. 50(3), 365-377.

    21. Optimization of Buffalo (Bubalus bubalis) Embryonic Stem Cell Culture System. Zandi M, Muzaffar M, Shah SM, Singh MK, Palta P, Kumar Singla S, Manik R and Chauhan MS. 2015


    Cell J. 17(2):264-73.

    22. Activation and inhibition of the Wnt3A signaling pathway in buffalo (Bubalus bubalis) embryonic stem cells: effects of WNT3A, Bio and Dkk1. Zandi M, Shah SM, Muzaffar M, Singh MK, Palta P, Singla SK, Manik RS and Chauhan MS. 2015


    International Journal of Fertility and Sterility.  9(3): 361-70.

    23. Developmental competence and expression pattern of bubaline (Bubalus bubalis) oocytes subjected to elevated temperatures during meiotic maturation in vitro. Ashraf S, Shah SM, Saini N, Dhanda S, Kumar A, Goud TS, Singh MK, Chauhan MS and Upadhyay RC. 2014


    Journal of assisted reproduction and genetics 31 (10), 1349-1360.

    24. Quantitative expression of pluripotency-related genes in parthenogenetically produced buffalo (Bubalus bubalis) embryos and in putative embryonic stem cells derived from them. Singh, K.P., Kaushik, R., Mohapatra, S.K., Garg, V., Rameshbabu, K., Singh, M.K., Palta, P., Manik, R.S., Singla, S.K. and Chauhan, M.S. 2014. 2014


    Gene Expression Patterns 16(1), 23-30.

    25. Construction of a recombinant human insulin expression vector for mammary gland-specific expression in buffalo (Bubalus bubalis) mammary epithelial cell line. Kaushik, R., Singh, K.P., Kumari, A., Rameshbabu, K., Singh, M.K., Manik, R.S., Palta, P., Singla, S.K. and Chauhan, M.S. 2014. 2014


    Molecular Biology Reports 41(9), 5891-5902.

    26. Buffalo (Bubalus bubalis) ES cell-like cells are capable of in vitro skeletal myogenic differentiation. M.K. Singh, K.P. Singh, D. Kumar, R.A. Shah, T. Anand, M.S. Chauhan, R.S. Manik, S.K. Singla and P. Palta. 2013. Reprod. Dom. Anim., 48(2):284-91.
    27. WNT3A signaling pathway in buffalo (Bubalus bubalis) embryonic stem cells. Zandi M,  Muzaffar M, Shah SM, Kaushik R, Singh MK,  Palta P, Singla SK, Manik RS  and Chauhan MS. 2013. 2013


    Reproduction, Fertility and Development: 26: 551-561.

    28. Effect of physiologically relevant heat shock on development, apoptosis and expression of some genes in buffalo (Bubalus bubalis) embryos produced in vitro. A. Yadav, K.P. Singh, M.K. Singh, N. Saini, P. Palta, R.S. Manik, S.K. Singla, R.C. Upadhyay and M.S. Chauhan. 2013. 2013


    Reproduction in Domestic Animals,  48(5): 858-865.

    29. Isolation, characterization, and EGFP expression in the buffalo (Bubalus bubalis) mammary gland epithelial cell line. Kaushik R, Singh KP, Kumari A, Singh MK, Manik RS, Palta P, Singla SK and Chauhan MS. 2013


    In Vitro Cellular & Dev Biol – Animal 49(1): 1-7.

    30. Effects of GDNF, FGF2 and EGF on proliferation and expression of some important genes in buffalo (Bubalus bubalis) spermatogonial cells. P.H. Kadam, S. Kala, H. Agrawal, K.P. Singh, M.K. Singh, M.S. Chauhan, P. Palta, S.K. Singla and  R.S. Manik. 2012


    Reproduction, Fertility and Development. 25: 1149-1157.

    31. Equivalency of buffalo (Bubalus bubalis) embryonic stem cells derived from fertilized, parthenogenetic and handmade cloned embryos. M. Muzaffar, N.L. Selokar, K.P. Singh, M. Zandi, M.K. Singh, R.A. Shah, M.S. Chauhan, S.K. Singla, P. Palta and R.S. Manik. 2012


    Cellular Reprogramming, 14(3):267-79.

    32. In vitro culture and morphological characterization of prepubertal buffalo (Bubalus bubalis) putative spermatogonial stem cell. Kala S, Kaushik R, Singh KP, Kadam PH, Singh MK, Manik RS, Singla SK, Palta P, Chauhan MS. 2012


    J. Assist. Reprod. Genet. 29(12): 1335-1342.

    33. Expression pattern of pluripotent markers in different embryonic developmental stages of buffalo (Bubalus bubalis) embryos and putative embryonic stem cells generated by parthenogenetic activation. Singh KP, Kaushik R, Garg V, Sharma R, George A, Singh MK, Manik RS, Palta P, Singla SK, Chauhan MS. 2012


    Cell. Reprogram. 14(6): 530-538.

    34. Oxygen concentration and cysteamine supplementation during in vitro production of buffalo (Bubalus bubalis) embryos affect mRNA expression of BCL-2, BCL-XL, MCL-1, BAX and BID. Elamaran G, Singh KP, Singh MK, Singla SK, Chauhan MS, Manik RS, Palta P. 2012


    Reprod. Domest. Anim. 47(6): 1027-1036.

    35. Derivation of buffalo embryonic stem-like cells from in vitro-produced blastocysts on homologous and heterologous feeder cells. Kumar, D., Anand, T., Singh, K.P., Singh, M.K., Shah, R.A., Chauhan, M.S. Palta, P., Singla, S.K., and Manik, R.S. 2011


    J Assist Reprod Genet., 28: 697-688.

    36. Buffalo (Bubalus bubalis) embryonic stem cell-like cells and preimplantation embryos exhibit comparable expression of pluripotency-related antigens. T. Anand, D. Kumar, M.K. Singh, R.A Shah, M.S. Chauhan, R.S. Manik, S.K. Singla and P. Palta. 2011


    Reprod. Dom. Anim. 6: 50-58.

    37. Effect of sodium nitroprusside, a nitric oxide donor, and aminoguanidine, a nitric oxide synthase inhibitor, on in vitro development of buffalo (Bubalus bubalis) embryos. S. Saugandhika, D. Kumar, M.K. Singh, R. Shah, T. Anand, M.S.Chauhan, R.S. Manik, S.K. Singla, P. Palta. 2010


    Reprod. Dom. Anim., 45 (5): 931-933.

    38. Pregnancies established from handmade cloned blastocysts reconstructed using skin fibroblasts in buffalo (Bubalus bubalis). R.A. Shah, A. George, M.K. Singh, D. Kumar, T. Anand, M.S. Chauhan, R.S. Manik, P. Palta and S.K. Singla. 2009  Theriogenology. 71(8):1215-9.
    39. Hand-Made Cloned Buffalo (Bubalus bubalis) Embryos: Comparison of Different Media and Culture Systems. R.A. Shah, A. George, M.K. Singh, D. Kumar, T. Anand, M.S. Chauhan, R.S. Manik, P. Palta and S.K. Singla. 2008


    Cloning and Stem Cells. 10 (4):435-42. (Now Cellular Reprogramming)

    Articles in Indian Referred Journals (07)
    40. Comparative study of three different media to improve semen processing and flow cytometric analysis in bovine. Nala N., Kumar V., Singh M.K., Manik R.S. and Singla S.K.





    Journal of Animal Research, 7(3): 537-541.

    41. Derivation, enrichment and characterization of goat (Capra hircus) spermatogonial stem cells from pre-pubertal testes. Sharma A, Shah SM, Saini N, Kaushik R, Singh MK, Manik RS, Singla SK, Palta P and Chauhan MS. 2016


    Indian J. Anim. Res., 50(5): 662-667.

    42. ‘Holi’: India’s first cattle calf produced through ovum pick-up – IVF technology- from an aged sahiwal cattle. Saini N, Singh MK, Singh KP, Manik RS, Singla SK, Palta P and Chauhan MS. 2015


    Indian J. Animal Sci. 85(3), 244-246.

    43. Expression of apoptosis related genes in buffalo embryos produced through in vitro fertilization and parthenogenetic activation. Singh, MK, Saw S, Singh KP, Saini N, Kaushik R, Manik RS, Palta P, Singla SK and Chauhan MS. 2014


    Indian Journal of Animal Sciences84 (2): 146–151.

    44. Characterization of the coding region of basic fibroblast growth factor (FGF2) c-DNA (open reading frame) in buffalo cumulus cells. Kumar S, Singh MK, Kumar V, Palta P, Singla SK, Manik RS and Chauhan MS. (2013). Indian Journal of Animal Sciences 83(7):744-746.
    45. Expression patterns of OCT-4 and NANOG genes in buffalo (Bubalus bubalis) embryos produced by in vitro fertilization or parthenogenetic activation. Kumar D, Anand T, Shah RA, Singh MK, Chauhan MS and Manik RS. 2012


    Ind. J. Anim. Sci. 82(8): 834-837.

    46. Generation of buffalo embryonic stem cell-like cells from in vitro produced, day 8 hatched and day 9 expanded blastocysts Kumar D, Anand T, Singh KP, Shah RA, Singh MK, Chauhan MS, Palta P, Singla SK and Manik RS. 2012


    Ind. J. Anim. Sci. 82(8): 838-843.

    Present Lab Members: Dr. M.K. Singh & Dr. P. Palta

    Students currently enrolled

    Sr. No. Name of student Class Dissertation topic:
    1. Mr. Gaurav Tripathi



    Ph.D. Scholar Effect of miRNA- 29b and miRNA- 449b   on buffalo transgenic embryos, containing human insulin gene, produced by handmade cloning.
    2. Ms. Tanya Gupta



    Ph.D. Scholar Global DNA methylation profile of spermatozoa of cloned buffalo bulls and their respective donor bulls.
    3. Ms. Kumari Rinka



    Ph.D. Scholar Effect of trichostatin A and 5-aza-2’-deoxycytidine on the developmental competence and transcriptome profile of buffalo embryos produced by Hand-made cloning”
    4. Ms. Sonal Gupta



    Ph.D. Scholar Effect of oxamflatin and ascorbic acid on the developmental competence and transcriptome profile of buffalo embryos produced by Hand- made cloning (Proposed).
    5.        Mr. Atul Kaushal



    Ph.D. Scholar Course work
    6.        Mr. Prem Kumar



    Ph.D. Scholar Course work
    7.        Mr. Faiz Akram



    M.Sc. Scholar Course work
    8.        Ms. Smriti Gupta



    M.Sc. Scholar Course work
    9.        Ms. Kanika Gandhi



    M.Sc. Scholar Course work
    10.    Ms. Ritika



    M.Sc. Scholar Course work

    Technologies Developed (if any):

    • Ovum pick-up-IVF technique standardised in indigenous cattle (India’s first cattle calf “Holi” produced)
    • Hand-guided Cloning protocol in buffalo developed.
    • Established the procedure of buffalo embryonic stem cell culture and their induced differentiation into myogenic cells.
    • Establishment of spermatogonial stem cell line in buffalo and goat.
    • Protocol established for transgenic embryo production in goat and buffalo.