• Dr. Rakesh Kumar

    Dr. Rakesh Kumar
    Principal Scientist

    Animal Biotechnology Centre

    • Current Research Area
      Functional Genomics, Gamete Biology, Non-coding RNAs, Reproductive Immunology


    Degree Name of the College/Institute and University Passing Year
    B.Sc.(Life Sciences) Himachal Pradesh University, Shimla, H.P. 1996
    M.Sc. (Biotechnology) Punjabi University, Patiala, India 1998
    Ph. D. (Biotechnology) Cochin University of Science & Technology, Kochi, India. 2009


    My research interests are in functional genomics related to buffalo oocytes and early embryos, circulatory miRNAs associated with pregnancy. I am also focus on molecular determinants of bull fertility, coding and non-coding carriage of extracellular vesicles and their carriages in seminal plasma in relation to bull fertility and heterologous expression of buffalo sperm coat proteins.


    S. No. Course code Course nomenclature Level
    1 BT-613 Immunology Applied to biotechnology (3+0) Masters
    2 BT-612 Animal Tissue culture: Principles and Applications (2+1) Masters
    3 BT-621 Applied Molecular Biology (3+0) Masters
    4 BT-623 Animal Genomics (2+1) Masters
    5 BT-712 Gene Cloning and Expression (3+0) PhD
    6    BT-722 Advances in Animal Tissue culture (3+0) PhD


    1. Batra, V. Avinash Maheshwarappa, Komal Dagar, Sandeep Kumar, Apoorva Soni, A. Kumaresan, Rakesh Kumarand T. K. Datta. 2019. Unusual interplay of contrasting selective pressures on β-defensin genes implicated in male fertility of the Buffalo (Bubalus bubalis). BMC Evolutionary Biology (2019) 19:214, https://doi.org/10.1186/s12862-019-1535-8.
    2. Kumar Manish, Faraji Mona, Parul, Kumar Sandeep, Gohain Moloya, De S, Kumar Rakesh and Datta TK. (2018) Propensity in low-grade oocytes for delayed germinal vesicle breakdown compromises the developmental ability of sub-optimal grade Bubalus bubalis Zygote. Accepted & in press.
    3. Panda Madhusmita, Asmita, Sandeep Kumar, Purusottam Mishra, Mahesh Chandra Sahu, Sachinandan De,Tirtha Kumar Datta, Rakesh Kumar. 2018. Effect of exogenous progesterone on cumulus characteristics of buffalo oocytes by allowing passage of more number of sperm through cumulus but not essentially fertilization. Asian Pacific Journal of Reproduction. doi: 10.4103/2305-0500.228017.
    4. Chaubey GK, Kumar S, Kumar M, Sarwalia Parul, Kumaresan, A, De S, Kumar R and Datta T K. (2018). Induced cumulus expansion of poor quality buffalo cumulus oocyte complexes by Interleukin-1beta improves their developmental ability. Journal of Cellular Biochemistry doi:10.1002/jcb.26688.
    5. Sandeep Kumar, Sunny Dholpuria, Gaurav Kumar Chaubey, Rakesh Kumar, and Tirtha Kumar Datta. (2018). Assessment of Nuclear Membrane Dynamics Using Anti-lamin Staining Offers a Clear Cut Evidence of Germinal Vesicle Breakdown in Buffalo Oocytes. Cytology and Genetics, 52, 80-85
    6. Kumar Sandeep, Kumar Manish, Dholpuria Sunny, Sarwalia Parul, Batra Vipul, De Sachinandan, Kumar Rakesh and Datta Tirtha Kumar. 2017. Transient arrest of Germinal Vesicle Breakdown improved in vitro development potential of buffalo (Bubalus bubalis) oocytes. Journal of Cellular Biochemistry. doi: 0.1002/jcb.26171.
    7. Sunny Dholpuria, Manish Kumar, Sandeep Kumar, Parul Sarwalia, Sandeep Rajput, Rakesh Kumar, Sachinandan De and Tirtha Kumar Datta. 2016. Differential expression of newly identified long intergenic non-coding RNAs in buffalo oocytes indicating their possible role in maturation and embryonic development. Journal of Cellular Biochemistry. doi: 10.1002/jcb.25828
    8. Kumar R, DK Datta, KV lalitha, 2015. Salmonella grows vigorously on seafood  and expresses its virulence and stress genes at different temperature exposure. BMC Microbiology, DOI 10.1186/s12866-015-0579-1.
    9. Jain, A, T. Jain, P. Kumar, M. Kumar, S. De, Gohain, R. Kumar, T.K. Datta, 2016. Follicle-stimulating hormone–induced rescue of cumulus cell apoptosis and enhanced development ability of buffalo oocytes. Domestic Animal Endocrinology, 55, 74-82
    10. Kumar P, Verma A, Kumar M, De S, Kumar R, Datta TK. 2015. Expression pattern of glucose metabolism genes correlate with development rate of buffalo oocytes and embryos in vitro under low oxygen condition. J Assist Reprod Genet. [Epub ahead of print].
    11. Verma A, Rajput S, Kumar S, De S, Chakravarty AK, Kumar R, Datta TK. 2014. Differential histone modification status of spermatozoa in relation to fertility of Buffalo bulls. J Cell Biochem. doi: 10.1002/jcb.25029. [Epub ahead of print]
    12. Verma A, Rajput S, De S, Kumar R, Chakravarty AK, Datta TK. 2014. Genome-wide profiling of sperm DNA methylation in relation to buffalo (Bubalus bubalis) bull Theriogenology. 2014 Sep 15;82(5):750-9.e1. doi: 10.1016/j.theriogenology.2014.06.012.
    13. Jena SC, Kumar S, Rajput S, Roy B, Verma A, Kumaresan A, Mohanty TK, De S, Kumar R, Datta TK. 2014. Differential methylation status of IGF2-H19 locus does not affect the fertility of crossbred bulls but some of the CTCF binding sites could  be potentially important. Mol Reprod Dev. 2014 Apr; 81(4):350-62. doi: 10.1002/mrd.22303.
    14.  Kumar R, K.V. Lalitha, 2013. Prevalence and Molecular Characterization of Vibrio cholerae O1, Non-O1 and Non-O139 in Tropical Seafood in Cochin, India. Foodborne Pathogens and Disease, 10, 278-283. doi:10.1089/fpd.2012.1310.
    15. Kumar R, K.V.Lalitha, 2012. Digoxigenin-labelled probe based colony blotting assay for rapid quantification of Salmonella serovars in seafood and water. Journal of AOAC International, 95, 1652-55