Dr K P Ramesha
Dr K P Ramesha
Southern Campus of NDRI Adugodi, Bangalore-560030 Telephone No.: 080-25710661-4 Ext 311
Current Research Area
Genomics, Cytogenetics, Animal Genetic Resources and IPR issues
Major Research Accomplishments
Developed “Zona free hamster egg bioassay for fertility evaluation of buffalo males For which I received Dr.G.B.Singh memorial Award.
We screened cattle and buffalo bulls for sperm chromosomal abnormality.
We reported the synergistic effect of pentoxifyline and heparin on buffalo sperm motility and in vitro fertilization of oocytes matured in culture which is useful to improve the success rate of IVF particularly in buffaloes.
We developed a Dual staining (Nigrosin. Eosin Giemsa) technique for simultaneous determination of viability and acrosomal status of bovine spermatozoa.
The molecular genetic studies in South Indian Zebu breeds revealed the genetic relationship between the breeds. Construction of dendrogram of relationship based on genetic distances clearly indicated that Hallikar breed is the progenitor for the draft breeds of southern India. Malnad Gidda is genetically distinct from other south Indian zebu breeds.
Characterization and evaluation of Murrah and South Kanara buffaloes using molecular markers was carried out by us.
We characterized Krishnavalley breed of cattle for economic traits. An effort was made to conserve the breed with the participation of progressive farmers and NGOs A total of 19000 doses of semen from elite Krishnavalley bulls were frozen for future use.
Our effort to identify the SNPs in ? lactalbumin and ?- s, casein genes indicated the utility of the study to identify and to predict the effect of SNPs on structure and function.
For the first time superior yak males were identified and male exchange programme was started by us to avoid inbreeding and for their genetic improvement. The molecular genetic studies clearly indicated that all the phenotypic types of yak found in India are genetically similar.
Association between IGF-1 genotypes with birth weight and body weight at different ages was investigated. The polymorphism in IGF-1 was associated with birth weight. Heterozygotes were superior to homozygotes.
The studies on variation in candidate genes indicated that genes related to growth and SRY gene (related to reproduction) are highly conserved in yaks.
Our observations on semen collection and embryo transfer experiments clearly indicated that these procedures have been successfully standardized in yak and conservation of yak genetic resources can be successfully adopted using semen and embryos.
A simple reliable Ssp1-PCR-RFLP assay to identify male mediated introgression based on mutation in SRY gene was developed for identification of paternal origin of hybrids.
The protocols used in cattle for freezing of semen was modified by us to suit for use in freezing of yak semen. Morphometrical dimensions of yak spermatozoa are much lower than other bovines.
Fast, sensitive and reliable PCR assay was developed by us for molecular diagnosis of B. bigemina in yak and its hybrids using primers specific for small subunit ribosomal RNA sequence and the assay was tested at field level. The developed assay was able to detect carrier animals.
Externally funded Projects: worked as P.I for NATP project entitled “SNPs in alpha lactalbumin and ?- s, casein genes in cattle and buffaloes”. I was also Co-PI for one DBT funded project on Zebu cattle of southern India and one NATP project on Dairy Production in India – a critical appraisal of policy and production. I completed one consultancy project for NDDB.
I developed well equipped molecular genetics laboratory at SRS of NDRI, Bangalore. Two scholars earned M.V.Sc in Animal Genetics & Breeding in the area of Molecular Genetics under my guidance. Worked as Advisory Committee member for 3 Ph.D and 2 M.V.Sc students. Worked has External Examiner PG programme for various Universities including TANUVAS, Chennai, KVAFSU, Bidar, Dharwad and Bangalore Agricultural University.
Guidance to student: 2 M.V.Sc scholars
- I received Fakrudhin Ali Ahemed Award, for the biennium 2004-2005 by Indian Council of Agricultural Research, New Delhi
- Invited by the president Jawaharlal Nehru Centre for Animal Science Research to be a visiting Fellow of JNCASR.
- Best paper award-2005 – of the Alumni Association of NDRI, Bangalore for the article entitled “Stem cells in animal agriculture” Ind. J. of Dairy & Bio Science. 2005.
- Won the best research poster award for “Genetic divergence study among Deoni breed of cattle using RAPD markers” Appannavar, M.M., Govindiah M.G. and Ramesha,K.P. (2003) at the National seminar on “Quality up gradation and safety aspects of dairy products” at Veterinary college, Bidar, Karnataka.
- Best paper award by the Alumni Association, NDRI, Adugodi, Bangalore-560030 for the paper entitled “Embryo Transfer Technology In India: Costs and Applications” published in Indian Journal of Dairy and Biosciences, Vol. 10, 1999.
- Best paper award-1994 – of the Alumni Association of NDRI, Bangalore for the article entitled “Perspectives of gene transfer in animal production” Ind. J. of Dairy & Bio Science. 5: 34-39.
- Dr. G.B. Singh memorial award 1993 of the Indian Society for study of animal reproduction (ISSAR) at the 12th convention of ISSAR on 13th January 1995 at Akola, Maharashtra for the research paper entitled “Zona free hamster egg penetration test for assessing fertility of Murrah buffalo bulls”. Buffalo Journal. 9 (3): 259-263.
- Executive Education Programme –Multi-disciplinary perspectives including techno- scientific management and S&T policy ,Kennedy School of Government, Harvard University, USA
- Nano-technology and Nano- materials, NIAS, Bangalore
- Fourth NIAS-DST Course for Senior Scientist-Administrators on “Multi-disciplinary Perspectives on Science and Technology”. National Institute of Advanced Studies, Bangalore
- Intellectual Property Rights and World Trade Organization ASCI, Hyderabad supported by DST
- Indo-UK Stem Cell Workshop NCBS &JNCASR, Bangalore
- International workshop in Cell biology-2004 Institute of Bio-informatics & Applied Biotechnology
- Nucleic acid probes and hybridization methods in diagnosis of animal diseases Madras Veterinary College, TANUVAS, Chennai-7.
- Computer applications in Animal Biotechnology NDRI, Karnal
- Indo-US CME programme on Genetic Risk Assessment St.John’s Medical College, Bangalore
- Animal models for biomedical research Indian Institute of Sciences, Bangalore
- Training course on Genetic Risk Assessment Centre for Biotechnology, Punjab University, Chandigarh.
- Recent advances in Livestock Breeding (Gametogenic and Cytogenetic) Madras Veterinary College, Chenna
Patents, Technology, Methodology, Genetic Stock, Variety, etc.:
- Gene bank submissions: 23 Genetic Stock Description: Described Krishnavalley, Amrithmahal and Malnad Gidda cattle
- Paternally inherited species specific ssp1-pcr-rflp assay for hybridization analysis in yak-cattle hybrid populations. 86:635-637. K.P.Ramesha, S. Das, M.A. Kataktalware, B.C. Saravanan, R. Pourouchottamane, S. Bandopadyaya, G. Krishnan and M. Sarkar Ind. Vet. J.
- Calves born from anestrus yak (Poephagus grunniens L.) using ovsynch and superovulation treatment. 23: 721-725. M. Sarkar , S. Borah, P. Chakraborty, B.C. Deka, B.C.Sharma, M. Raquib, K. Baruah, K.P. Ramesha, M. Ghosh, R. Pourochottamne, M. Kataktakware, B.S.Prakash and M. Bhattacharya
- SNPs in alpha lactalbumin gene in zebu cattle of India. 76(10):816-820 ,Ramesha,K.P., Shivraj Gowda, Nagasuma R.Chandra and Rao, M.R.S. Ind. J.Anim.Sci.
- Isolation of an oocyte stimulatory peptide from the ovarian follicular fluid of water buffalo (Bubalus bubalis).18(9),Gupta, P.S.P., Ravindra,J.P., Nandi,S., Raghu, H.M. and Ramesha K.P. Asian Australian Journal of Animal Sciences.
- Multitrait selection with restriction for cutup carcass value: Genetic relatedness of lines involved based on RAPD. Vol. 18: 1535-1541. Kosravinia,, H. Murthy, H.N.N. Ramesha K.P. and Govindaiah, M.G. Asian Australian J.of Animal Sciences.
- Genetic distance study among Deoni breed of cattle using RAPD markers.15 (7):1-4. Appananavar, M.M., Govindiah,M.G. and Ramesha.K.P. Asian Australian J.Anim. Sci.
- Genetic distance among South Indian breeds of Zebu cattle using Random Amplified DNA Markers. 15(3): 309-314. Ramesha, K.P, Sarvanan, T, Rao, M.K. Appananavar, M.M and Obi Reddy, A. Asian Australian J. Anim Sci.
- Additive effect of Pentoxifyline and Heparin on buffalo sperm motility and fertilization of oocytes matured in culture. 16 (1):63-72. Ramesha,K.P, Balakrishnan,M, Krishnamurthy, L, and Balakrishnan, C.R. Buffalo Journal
- Sperm chromosome analysis of Karan Swiss and Karan Fries crossbred bulls. 37: 291-298.Ramesha,K.P. and Goswami,S.L. Anim. Reprod. Sci
- Chromosome analysis of Murrah buffalo (Bubalus bubalis) spermatozoa using zona free hamster eggs. 42: 297-305. Ramesha,K.P. and Goswami,S.L. Theriogenology