• Dr. Jai K. Kaushik

    Qualification:

    Degree Name of the College/Institute and University

    Passing Year

    PDF (JSPS) Inst for Protein Res, Osaka University, Japan

    2000-02

    PhD (Biotechnology) Jawaharlal Nehru University, New Delhi

    1997

    MSc (Biotechnology) Jawaharlal Nehru University, New Delhi

    1990

    BSc University of Delhi, Delhi

    1987

     

    Research:

    Major area of work: Gene cloning and recombinant protein expression, Biophysical chemistry, Structural Biology, Proteomics. Proteins are produced by DNA recombinant technology by optimizing their production in biologically active form or by refolding them from the inclusion bodies. Their structure and function are studis by using various biochemical and biophysical methods including spectroscopic (Circular dichroism, Fluorescence spectroscopy) and thermodynamic techniques (DSC) for understanding the kinetics and thermodynamics associated with molecular processes. Also, we produce high throughput transcriptomics and proteomics data for understanding the molecular and cellular processes and the underlysing mechanisms.
    Antimicrobial proteins: We are working on animal originated antimicrobial proteins like lysozyme, lactoferrin, lactadherin, xanthine oxidoreductase and genes/proteins involved in molybdenum cofactor (Moco) synthesizing metabolic pathway. We are also targeting the antimicrobial resistance via engineering the bacteriophage originated endolysins to improve their activity against the gram negative pathogens, since these organisms are impervious owing to their outer membrane. We are also interested in proteins of probiotic origin like surface proteins to serve as antimicrobial agent against drug-resistant microbes.

    Figure shows the differences in the FAD and NAD binding sites between cattle (A) and buffalo (B) xanthine oxidoreductase (Kaustubh et al., 2012, PLOS ONE).

    Proteolytic enzymes: Another area is production of recombinant aminopeptidases (PepL, PepI, PepN) and other proteolytic enzymes (cell lenvelope protease PepO2) of lactic acid bacteria.  Recombinant leucyl aminopeptidase (PepL) has been found very active but possesses lower thermal stability. Currently, we are trying to engineer PepL to enhance its thermal stability. These enzymes could find an application in dairy, food and pharmaceutical industry for the production of protein hydrolysate or production of bioactive peptides.

    Figure: Active site of PepL of Lactobacillus casei. The balls in magenta color indicate cobalt ion.

    Protein associated with animal reproduction and health: We also have interest in understanding the important genes/proteins involved in animal reproduction, e.g., those involved in sperm-oocyte binding like sperm lysozyme-like proteins (SLLP) and their receptors, and fertility augmenting factors like growth and differentiation factor (GDF9). We try to understand the role of proteins in binding of sperm with oocytes by using biophysical and structural modeling work. The work should provide a better understanding of sperm-oocyte binding and possible reason and treatment of infertility arising because of those reasons in farm animals.

    Figure: The sperm protein (shown in yellow color) binds in the active site of the receptor localized on the oocyte. The receptor has been shown in grey color with zinc (shown in magenta color) sitting in its active site channel.

    Animal Proteomics: Transcriptomics, proteomic and other studies are important to understand the dynamics and adatation at system level in response to external/internal stimuli or genetic variations. We are analysing the proteome and transcriptome of farm animals to understand production and reproduction processes. Our interest is to understand spermatogenesis process and expression of X and Y chromosome located genes; factors responsible for semen freezing potential in cross-bred bulls, and pashmina fibre growth and development to name a few by analysing the differential transcriptome and proteome. The study is likely to provide important biomarkers, biosynthetic pathways and biological processes involved in gene expression regulation at transcrition and protein level.

    Teaching:

    S. No.

    Course code

    Course nomenclature

    Level

    1.

    BT-711

    Gene cloning and expression

    PhD

    2.

    BT-712

    Functional genomics and proteomics

    PhD

    3.

    BT-621

    Applied Molecular Biology

    MSc

    4.

    BT-624

    Techniques in molecular biology and genetic engineering

    MSc

    Publications:

    1. Bathla S, Sindhu A, Kumar S, Dubey SK, Pattnaik S, Rawat P, Chopra A, Dang A, Kaushik JK, Mohanty AK.(2020). Tandem Mass Tag (TMT)-based quantitative proteomics reveals potential targets associated with onset of Sub-clinical Mastitis in cows. Sci Rep. 10: 9321. doi: 10.1038/s41598-020-66211-6
    2. Jaswal S, Anand V, Kumar S, Bathla S, Dang AK, Kaushik JK, Mohanty AK. (2020). In-depth proteome analysis of more than 12,500 proteins in buffalo mammary epithelial cell line identifies protein signatures for active proliferation and lactation. Sci Rep. 10: 4834. doi: 10.1038/s41598-020-61521-1.
    3. Choudhary, S., Janjanam, S., Kumar, S., Kaushik, J.K., Mohanty, A.K. (2019). Structural and functional characterization of buffalo oviduct-specific glycoprotein (OVGP1) expressed during estrous cycle. Bioscience reports, 39 (12).
    4. Singh, S., Kalra, S., Bubber, P., Datta, T.K., Mohanty, A.K., Kaushik, J.K. (2019). Functional analysis of recombinant buffalo lactoferrin and monoferric lobes and their cytotoxic effect on buffalo mammary epithelial cells. BioMetals 32 (5): 771-783.
    5. Singh, S., Choudhary, S., Anand, V., Jaswal, S., Verma, A.K., Kumar, S., Kaushik, J.K., Mohanty, A.K. (2019). New insights into the catalytic inactivity of mammary gland protein-40, a chitinase-like protein expressed during mammary gland involution. Molecular Biology Reports 46 (2): 2243-2257.
    6. Lotfan, M. Choudhary, S., Yadav, M.L., Tripathi, I., Bhan, S.S, Bathla, S., Rawat, P., Kumar, S., Mohanty, T.K., Kaushik, J.K., Mohanty, A.K. (2019). Characterization of buffalo native pregnancy-associated glycoprotein: mass spectrometry-based glycan composition analysis, sugar-binding characteristics and proteolytic. Journal of Proteins and Proteomics 10 (1): 23-32.
    7. Bhat, B., Singh, A., Iqbal, Z., Kaushik, J.K., Rao, A.R., Ahmad, S.M., Bhat, H.,  Ayaz, A., Sheikh, F.D., Kalra, S., Shanaz, S., Mir, M.S., Agarwal, P.K., Mohapatra, T., Ganai, N.A. (2019). Comparative transcriptome analysis reveals the genetic basis of coat color variation in Pashmina goat. Scientific Reports 9 (1): 1-9.
    8. Singh, K.S., Kumar, S., Mohanty, A.K., Grover, S., Kaushik, J.K. (2018). Mechanistic insights into the host-microbe interaction and pathogen exclusion mediated by the Mucus-binding protein of Lactobacillus plantarum. Scientific Reports 8 (1): 1-10
    9. Ali, S., Malakar, D., Kaushik, J.K., Mohanty, A.K., Kumar, S. (2018). Recombinant purified buffalo leukemia inhibitory factor plays an inhibitory role in cell growth. PloS ONE 13 (6), e0198523.
    10. Bisht, S., Singh, K.S., Choudhary, R., Grover, S., Kumar, S., Mohanty, A.K., Kaushik, J.K. (2018). Expression of fibronectin-binding protein of L. acidophilus NCFM and in vitro refolding to adhesion capable native-like protein from inclusion bodies Protein Expression & Purification, 145: 7-13. doi: 10.1016/j.pep.2017.11.007. PMID: 29229289.
    11. Ali, S.A., Kaur, G., Kaushik, J.K., Malakar, D., Mohanty, A.K., Kumar, S. (2017). Examination of pathways involved in leukemia inhibitory factor (LIF)-induced cell growth arrest using label-free proteomics approach. Journal of Proteomics, 168: 37-52. doi: 10.1016/j.jprot.2017.07.014. PMID: 28755912
    12. Choudhary, S., Kumaresan, A., Kumar, M., Chhillar, S., Malik, H., Kumar, S., Kaushik, J.K., Datta, T.K., Mohanty, A.K. (2017). Effect of recombinant and native buffalo OVGP1 on sperm functions and in vitro embryo development: a comparative study. Journal of Animal Science & Biotechnology, 8: 69-80. DOI 10.1186/s40104-017-0201-5. PMID: 28883914
    13. Singh, K.S., Choudhary, R., Bisht, S., Grover, S., Kumar, S., Mohanty, A.K. and Kaushik, J.K. (2017). Expression of recombinant truncated domains of mucus-binding (Mub) protein of Lactobacillus plantarum in soluble and biologically active form. Protein Expression & Purification, 135: 54-60. https://doi.org/10.1016/j.pep.2017.04.015. PMID: 28499579
    14. Chaudhari, D.D., Singh, R., Mallappa, R.H., Rokana, N., Kaushik, J.K., Bajaj, R.,  Batish, V.K., Grover, S. (2017). Evaluation of casein & whey protein hydrolysates as well as milk fermentates from Lactobacillus helveticus for expression of gut hormones. The Indian Journal of Medical Research. 146 (3): 409.
    15. Kaur, G., Ali, S.A., Pachauri, S., Malakar D., Kaushik, J.K., Mohanty A.K., Kumar S. (2017). Buffalo Leukemia Inhibitory Factor Induces Differentiation and Dome-Like Secondary Structures in COS-1 Cells. Cytogenetics & Genome Research. 151: 119-130. https://doi.org/10.1159/000465507. PMID: 28441662
    16. Tyagi, A., Kumar, A., Mohanty, A.K., Kaushik, J.K., Grover, S., Batish, V.K. (2017). Expression of buffalo chymosin in Pichia pastoris for application in mozzarella cheese. LWT-Food Science and Technology.

    Book Chapters:

    1. R. Kumar & J. K. Kaushik. (2015). Molecular Biology of adaptation of Starter lactic acid Bacteria to dairy System. In Fermented Milk and Dairy Products Eds. A. K. Puniya, CRC Press, Pp: 133-166.
    2. J. K. Kaushik (2009). Structure and Stability of Proteins from Hyperthermophiles. In Advances in Microbiology, Chemistry & Technology of Dairy Functional Foods & Nutraceuticals. Editors: R. Singh, S. K. Tomar, G. Goel (Eds). ISBN 81-8321-009-0. Agrotech Publishing Academy, Udaipur, India. Pp. 100-107.
    3. J. K. Kaushik (2011). Adaptation at Molecular Level in Hyperthermophiles. In Functional Dairy Foods: Concepts and Applications. S. K. Tomar, R. Singh, A. K. Singh, S. Arora, R. R. B. Singh (Eds). ISBN: 81-89304-90-9. Satish Serial Publishing House, New Delhi, India. Pp 183-189. 

    Technologies Developed/ Patents Granted (if any):
    Patent: Method for regulating fertility (Submitted)

    Dr. Rakesh Kumar

    Qualification:

    Degree Name of the College/Institute and University Passing Year
    B.Sc.(Life Sciences) Himachal Pradesh University, Shimla, H.P. 1996
    M.Sc. (Biotechnology) Punjabi University, Patiala, India 1998
    Ph. D. (Biotechnology) Cochin University of Science & Technology, Kochi, India. 2009

    Research:

    My research interests are in functional genomics related to buffalo oocytes and early embryos, circulatory miRNAs associated with pregnancy. I am also focus on molecular determinants of bull fertility, coding and non-coding carriage of extracellular vesicles and their carriages in seminal plasma in relation to bull fertility and heterologous expression of buffalo sperm coat proteins.

    Teaching:

    S. No. Course code Course nomenclature Level
    1 BT-613 Immunology Applied to biotechnology (3+0) Masters
    2 BT-612 Animal Tissue culture: Principles and Applications (2+1) Masters
    3 BT-621 Applied Molecular Biology (3+0) Masters
    4 BT-623 Animal Genomics (2+1) Masters
    5 BT-712 Gene Cloning and Expression (3+0) PhD
    6    BT-722 Advances in Animal Tissue culture (3+0) PhD

     Publications:

    1. Batra, V. Avinash Maheshwarappa, Komal Dagar, Sandeep Kumar, Apoorva Soni, A. Kumaresan, Rakesh Kumarand T. K. Datta. 2019. Unusual interplay of contrasting selective pressures on β-defensin genes implicated in male fertility of the Buffalo (Bubalus bubalis). BMC Evolutionary Biology (2019) 19:214, https://doi.org/10.1186/s12862-019-1535-8.
    2. Kumar Manish, Faraji Mona, Parul, Kumar Sandeep, Gohain Moloya, De S, Kumar Rakesh and Datta TK. (2018) Propensity in low-grade oocytes for delayed germinal vesicle breakdown compromises the developmental ability of sub-optimal grade Bubalus bubalis Zygote. Accepted & in press.
    3. Panda Madhusmita, Asmita, Sandeep Kumar, Purusottam Mishra, Mahesh Chandra Sahu, Sachinandan De,Tirtha Kumar Datta, Rakesh Kumar. 2018. Effect of exogenous progesterone on cumulus characteristics of buffalo oocytes by allowing passage of more number of sperm through cumulus but not essentially fertilization. Asian Pacific Journal of Reproduction. doi: 10.4103/2305-0500.228017.
    4. Chaubey GK, Kumar S, Kumar M, Sarwalia Parul, Kumaresan, A, De S, Kumar R and Datta T K. (2018). Induced cumulus expansion of poor quality buffalo cumulus oocyte complexes by Interleukin-1beta improves their developmental ability. Journal of Cellular Biochemistry doi:10.1002/jcb.26688.
    5. Sandeep Kumar, Sunny Dholpuria, Gaurav Kumar Chaubey, Rakesh Kumar, and Tirtha Kumar Datta. (2018). Assessment of Nuclear Membrane Dynamics Using Anti-lamin Staining Offers a Clear Cut Evidence of Germinal Vesicle Breakdown in Buffalo Oocytes. Cytology and Genetics, 52, 80-85
    6. Kumar Sandeep, Kumar Manish, Dholpuria Sunny, Sarwalia Parul, Batra Vipul, De Sachinandan, Kumar Rakesh and Datta Tirtha Kumar. 2017. Transient arrest of Germinal Vesicle Breakdown improved in vitro development potential of buffalo (Bubalus bubalis) oocytes. Journal of Cellular Biochemistry. doi: 0.1002/jcb.26171.
    7. Sunny Dholpuria, Manish Kumar, Sandeep Kumar, Parul Sarwalia, Sandeep Rajput, Rakesh Kumar, Sachinandan De and Tirtha Kumar Datta. 2016. Differential expression of newly identified long intergenic non-coding RNAs in buffalo oocytes indicating their possible role in maturation and embryonic development. Journal of Cellular Biochemistry. doi: 10.1002/jcb.25828
    8. Kumar R, DK Datta, KV lalitha, 2015. Salmonella grows vigorously on seafood  and expresses its virulence and stress genes at different temperature exposure. BMC Microbiology, DOI 10.1186/s12866-015-0579-1.
    9. Jain, A, T. Jain, P. Kumar, M. Kumar, S. De, Gohain, R. Kumar, T.K. Datta, 2016. Follicle-stimulating hormone–induced rescue of cumulus cell apoptosis and enhanced development ability of buffalo oocytes. Domestic Animal Endocrinology, 55, 74-82
    10. Kumar P, Verma A, Kumar M, De S, Kumar R, Datta TK. 2015. Expression pattern of glucose metabolism genes correlate with development rate of buffalo oocytes and embryos in vitro under low oxygen condition. J Assist Reprod Genet. [Epub ahead of print].
    11. Verma A, Rajput S, Kumar S, De S, Chakravarty AK, Kumar R, Datta TK. 2014. Differential histone modification status of spermatozoa in relation to fertility of Buffalo bulls. J Cell Biochem. doi: 10.1002/jcb.25029. [Epub ahead of print]
    12. Verma A, Rajput S, De S, Kumar R, Chakravarty AK, Datta TK. 2014. Genome-wide profiling of sperm DNA methylation in relation to buffalo (Bubalus bubalis) bull Theriogenology. 2014 Sep 15;82(5):750-9.e1. doi: 10.1016/j.theriogenology.2014.06.012.
    13. Jena SC, Kumar S, Rajput S, Roy B, Verma A, Kumaresan A, Mohanty TK, De S, Kumar R, Datta TK. 2014. Differential methylation status of IGF2-H19 locus does not affect the fertility of crossbred bulls but some of the CTCF binding sites could  be potentially important. Mol Reprod Dev. 2014 Apr; 81(4):350-62. doi: 10.1002/mrd.22303.
    14.  Kumar R, K.V. Lalitha, 2013. Prevalence and Molecular Characterization of Vibrio cholerae O1, Non-O1 and Non-O139 in Tropical Seafood in Cochin, India. Foodborne Pathogens and Disease, 10, 278-283. doi:10.1089/fpd.2012.1310.
    15. Kumar R, K.V.Lalitha, 2012. Digoxigenin-labelled probe based colony blotting assay for rapid quantification of Salmonella serovars in seafood and water. Journal of AOAC International, 95, 1652-55

    Dr. Satish Kumar

    Qualification:

    Name of the degree University/Institution Passing Year
    B.Sc. (Ag & A.H.) GBPUA&T Pantnagar, Nainital, UP, India 1995
    M.Sc. (Animal Biotechnology) National Dairy Research Institute Karnal, Haryana India 1997
    Ph.D. (Animal Biotechnology) National Dairy Research Institute Karnal, Haryana India 2012
    Post-Doctoral Training (Molecular Genetics) Roslin Institute & Royal (Dick) School of Veterinary Studies, The University of Edinburgh, Scotland, UK 2013

    Research:

    My research interest is to elucidate the functional role of bone morphogenetic protein receptor (BMPR-1B) in sheep granulosa cells to understand the mechanism of higher ovulation rate in prolific sheep. Further, I am interested to edit β-lactoblobulin protein, and other milk associated genes in buffalo and goat through CRISPR-Cas9 mediated genome editing technologies.

    Teaching:

    S. No.   Course code Course nomenclature Level
    1 BT-713 Advance in Reproductive Biotechnology (3+0) PhD
    2 BT-622 Molecular Diagnostics (1+2) Masters
    3 BT-722 Advances in Animal Cell Culture (3+0) PhD
    4 BT-614 Reproductive Biotechnology (2+1) Masters
    5 BT-623 Animal Genomics (2+1) Masters

     

    Publication:

    • Vijay Kumar, Praveen Kumar, Satish Kumar, and Prakash BS. 2018. Seasonal alterations in plasma cortisol in peripartum Murrah buffaloes (Bubalus bubalis) as assayed by a sensitive enzymeimmunoassay in subtropical climate. Biological Rhythm Research. DOI:  10.1080/09291016.2018.1526497.
    • Kumar A, Sawal RK, Narula HK, Kumar S, Kumar R. 2018. Subjective and objective /machine evaluation of wool luster in Magra sheep vis a vis wool grading and animal selection. Journal of Natural Fibres. Doi.org./10.1080/15440478.2018.1431996.
    • Rajiv Kumar, Gadekar YP, Shinde AK, Naqvi SMK, and Kumar S. PCR based test for identification of ovine, caprine and bovine origin of meat sample. Indian Journal of Small Ruminants. 24 (1): 121-123.
    • Meena AS, Bhatt RS, Sahoo A, and Kumar S. 2017. Polymorphism of the exon 3 of leptin gene in Malpura sheep. Indian Journal of Animal Research. 51(3): 469-473.
    • Kumar R, Kumar S, Swarnkar CP, and Singh D. 2016. Allelic variant analysis of MHC DRB-1 coding region in Garole: The prolific sheep of India. Indian Journal of Biotechnology 15:507-510.
    • Paswan C, Prince LLL, Kumar R, Swarnker CP, Singh D, and Kumar S. 2016. Molecular characterization of Ovar-DRB-1 exon 2 gene in Garole sheep resilient to gastrointestinal nematode. Indian Journal of Animal Research. 50 (2): 143-147.
    • Jyotsana B, Kumar R, Kumari R, Meena AS, Prince LLL, Prakash V, Kumar S. 2016. Genetic variants of β-lactoglobulin in Garole, Malpura, Patanwari, and their crossbred sheep. Indian Journal of Small Ruminants. 22(2): 174-177.
    • Kumar R, Gupta S, Meena AS, Naqvi SMK, and Kumar S. 2016. Polymorphism in exon 17 of diacylglycerol acyltransferase-1 (DGAT-1) gene in Indian sheep breeds. Indian Journal of Small Ruminants. 22(2): 170-173.
    • Meena AS, Kumar R, Rajni K, Jyotsana B, Prince LLL, Kumar S. 2016. Aromatase gene polymorphism in sheep breeds of India. Indian Journal of Small Ruminants. 22(2): 227-229.
    • Kumar R, Shakawar DB, Pareek PK, Raja ASM P, Prince LLL, Kumar S, Naqvi SMK. 2015. Development of PCR-Based techniques for detection of purity of Pashmina fiber from textile materials. Applied Biochemistry and Biotechnology 175 (8): 3856-3862.
    • Kumar R, Meena AS, Kumari R, Jyotsana B, Prince LLL, and Kumar S. Polymorphism of KRT1.2 and KAP1.3 genes in Indian sheep breeds. Indian Journal of Small Ruminant. 22(1):28-31.
    • Meena AS, Bhatt RS, Sahoo A, and Kumar S. 2016. Genetic polymorphism of the diacylglycerol acyl transferase1 (DGAT1) gene in Malpura. Indian Journal of Small Ruminant. 22(1):97-99.
    • Share MG, Sawaimul AD, Ali SZ, Sirothia AR, and Kumar S. 2015. RFLP analysis of mitochondrial D-loop region in Indian sheep breeds. Indian Journal of Small Ruminants. 21(1): 13-16.

    Technologies Developed/ Patents Granted (if any):

    •  Developed PCR based method for identification of cashmere (Pashmina) fibre from processed textile products (ICAR patent granted. No. 3400/DEL/2015)
    • For the first time, Booroola fecundity (FecB) gene was discovered in Kendrapada sheep of India
    • Developed prolific sheep strains for higher litter size and growth rate
    • Developed resistant and susceptible Malpura and Avikalin sheep lines against Haemonchus contortus parasite
    • Developed DNA test for identification of benzimidazole resistance in Haemonchus contortus of sheep
    • Developed buffalo transgenic fetal fibroblast cells and embryonic stem cells (ES) cells for NANOG, LIF and FGF2 genes.

    Dr Shilpa Vij

    Qualifications:

    • PhD Dairying Dairy Microbiology, 1995, ICAR-National Dairy Research Institute
    • Sc. Dairying (Quality control) 1987, ICAR-National Dairy Research Institute
    • Sc. 1984, Kurukshetra University (KUK).

    Research:

     My lab is Functional Fermented Foods and Bioactive Peptides Lab. Our lab is actively engaged in the research and development of functional fermented foods and bioprocessing of whey into value-added products. Processes have been standardized for various probiotic fermented dairy and non-dairy foods and whey based products. The technologies have been developed for biofunctional synbiotic fruit yoghurts, soy-based yoghurt, soy dahi and whey based functional probiotic beverages. The laboratory is also engaged in the production of antimicrobial bioactive peptides from milk protein like whey and casein, and also from soy proteins and biopreservation of dairy products. Besides, the laboratory is also involved in the research project on the production of bioethanol from whey using stress tolerant yeasts. The research on food safety aspect has also been initiated.

    Research Projects:

    • Whey to Biofuel: Bioethanol Production by Stress Tolerant and Metabolically Engineered Yeast from Whey 2011-13
    • Production of antimicrobial bioactive peptides from bovine and non-bovine milk by microbial fermentation and simulated gastrointestinal digestion for development of functional ingredients  2015-2018
    • Production of Antimicrobial Bioactive Peptides from Milk Proteins by   Proteolytic Lactobacillus2008-2012
    • Isolation of antifungal bioactive proteins and peptides derived from milk and their application in biopreservation spp. 2004-2008
    • Micro-encapsulation Methods For Bacteriocins for their Controlled Release 2011-15
    • Development of Bacteriocin Based Anti Microbial Packaging (AMP) System for Enhanced     Food Safety 2008-2010
    • Development of biofunctional ingredients by enzymatic modification of whey protein concentrates. 2007-2009
    • Preparation of Micro/nano Emulsion for the Encapsulation of Bioactive Components.   2011-2013

    Major Research Accomplishments

    • Developed a process for the production of bioethanol from whey by stress tolerant yeasts.
    • Developed a method for the production of antimicrobial bioactive peptides from whey and casein.
    • Developed a process for control release of an antimicrobial substance by microencapsulation.
    • Developed a process for the production of bioethanol from by metabolic engineering of yeast.
    • Developed a process for co-immobilization of yeast and enzyme for ethanol production from whey.
    • Isolated antifungal bioactive proteins and peptides derived from milk and their application in biopreservation.
    • Development of concentrated inoculum of lactic cultures and its packaging in the flexible sachet for the preparation of fermented dairy products.
    • Development of bacteriocin based antimicrobial packaging (AMP) system for enhanced food safety.
    • Production and characterization of proteinaceous antifungal substances (AFS) from Lactobacillus spp.
    • Preservation of paneer by antifungal substances of Lactobacillus and antimicrobial proteins.
    • Antimicrobial activity of casein fermentate of probiotic Lactobacillu spp.

    Teaching :

    • Courses DM 321, DM615, DM626, FS614,DM712,DM721, DM723

    Guidance to Students (M.Sc and Ph.D.) :

    M.Sc completed:12

    Ph.D. completed:9

    Ph.D. ongoing:5

    M.Sc ongoing:2

    Awards:

    • Associate fellow award NADSI, 2018
    • Best Paper Award – in Indian J. Dairy Science in 2010 at XL Dairy Industry Conference organized by Indian Dairy  Association in New Delhi.
    • Best Paper award at XIL Dairy Industry Conference organized by Indian Dairy Association in Chennai from 12-14th 2013
    • Best Poster award – at XL Dairy Industry Conference organized by Indian Dairy Association in New Delhi.
    • Best Poster award – at National Symposium on Emerging Trends in Biotechnology organized by Dept. of Biotechnology, MMM College, Patiala, Punjab
    • Best Poster award (3rdPrize) in International Conference on Food Technology: Impact on Nutrition and Health, Sponsored by ICMR, DST, and DBT.
    • Best Poster award (3rdPrize) in International Conference on Food Technology: Impact on Nutrition and Health, at New Delhi on December 23-24, 2013 at Jawaharlal Nehru University, New Delhi
    • Best poster award (2ndplace) on Physiological growth profile of the dairy yeast Kluyveromyces marxianus 6C17 on galactose during batch cultivation in 44th Dairy industry conference held at ICAR, NDRI, Karnal during 18-20 Feb 2016.
    • Best Poster Award in National Conference on Advances in Food Science and Technology (AFST-2017) held at Eternal University, Baru Sahib, and Himachal Pradesh. (24-25 March 2017).
    • Publications:
    • Brij Pal Singh, Shilpa Vij(2018) In vitro stability of bioactive peptides derived from fermented soy milk against heat treatment, pH and gastrointestinal enzymes. LWT-Food Science and Technology, 91 303-307.
    • Priyanka Saini, Arun Beniwal, Anusha Kokkiligadda, Shilpa Vij(2018) Response and tolerance of yeast to changing environmental stress during ethanol fermentation. Process Biochemistry.  72, 1-12
    • Urmila Choudhary, Latha Sabikhi, Shaik Abdul Hussain, Kaushik Khamrui, Vivek Sharma, Shilpa Vij(2018) Stabilizing the primary emulsion with hydrophobic emulsifiers and salt for encapsulating herbal extracts in a double emulsion. J Food Process Preserv.  1 – 9 https://doi.org/10.1111/jfpp.13699
    • Bachanti, Prashant; Vij, Shilpa; Patil, M. R.; Bajad, D. N.; Adil, Shaikh; Kalyankar, S. D.(2018)Antimicrobial activity of casein fermentate of probiotic Lactobacillus spp.  Asian Journal of Dairy & Food Research., Vol. 37 Issue 3, 175-181. 7p. 2
    • Saini P., Beniwal A., and Vij S Evolutionary adaptation of Kluyveromyces marxianus strain for efficient conversion of whey lactose to bioethanol.Process Biochemistry 57 https://doi.org/10.1016/j.procbio.2017.07.013
    • Saini P., Beniwal A., Kokkiligadda A, and Vij S. 2017. Physiological response of Kluyveromyces marxianusduring oxidative and osmotic stress. Process Biochemistry 56, 21–29.
    • Saini P., Beniwal, A., and Vij, S. Comparative analysis of oxidative stress during aging of Kluyveromyces marxianusin synthetic and whey media. Applied Biochemistry and Biotechnology. 182: 1-14.
    • Saini P., Beniwal, A., and Vij, S. Comparative physiology of Kluyveromyces marxianusand Saccharomyces cerevisiae during batch cultivation on glucose as a sole carbon source. Indian Journal of Dairy Science. 70 (3)
    • Kokkiligadda, A., Beniwal, A., Saini, P., and Vij, S.(2016). Utilization of Cheese Whey Using Synergistic Immobilization of β-Galactosidase and Saccharomyces cerevisiaeCells in Dual Matrices. Applied Biochemistry and Biotechnology 179:1469-1484.
    • Subrota Hati1, Shilpa Vijand Surajit Mandal (2016) Selection of Lactic Dahi Cultures for the Fermentation of Soy Milk. EC Nutrition 5(5): 1248-1254.
    • Deependra Singh, Shilpa Vijand Brij Pal Singh (2016) Antioxidative and antimicrobial activity of whey based fermented soy beverage with curcumin supplementation. Indian Journal of Dairy Science. 69 No. 2; 171-177.
    • Shital Borkar, Shilpa Vijand Subrota Hati (2015) Antimicrobial activity of whey fermentate against antibiotic-resistant pathogens. Indian Journal of Dairy Science 68 No. 6, 577-583.
    • Bachanti P. R.  and Shilpa Vij(2015) Antimicrobial activity of sodium caseinate fermentate of Lactobacillus fermentum NCDC 141.Asian J. Dairy & Food Res, 34(4):265-269
    • Priyanka Kumari and Shilpa Vij(2015) Growth and antimicrobial activity of proteolytic probiotic Lactobacillus rhamnosus C6 in soymilk and whey Indian J. Dairy Sci. 68(3), 229-238.
    • Brij Pal Singh, Shilpa Vij, Subrota Hati, Deependra Singh, Priyanka Kumari and Jagrani Minj (2015). Antimicrobial activity of bioactive peptides derived from fermentation of soy milk by Lactobacillus plantarum C2 against common foodborne pathogens.  International Journal of Fermented Foods: 4(1) p1-9.
    • Subrota Hati, Shilpa Vij(2014) Evaluation of Probiotic potentials of cheese isolate  plantarum C6. Indian Journal of Dairy Science Vol 67, No 5 413-420
    • Narsaiah , Robin A. Wilson , K. Gokul, H.M. Mandge , S.N. Jha, Sheetal Bhadwal, Rahul K. Anurag , R.K. Malik , S. Vij  (2014) Effect of bacteriocin-incorporated alginate coating on shelf-life of minimally processed papaya (Carica papaya L.). Postharvest Biology and Technology 100 212–218.
    • Vandna, Shilpa Vijand Subrota Hati 2014 Physico-chemical and sensory quality of probiotic fermented whey drinks and its storage study Indian J Dairy Science Vol.67 No.2 132-138.
    • Hati S, S Vij,BP Singh, S Mandal  2014  β-Glucosidase activity and bioconversion of isoflavones during fermentation of soymilk.  Journal of the Science of Food and Agriculture first published on 6 June 2014 doi: 10.1002/jsfa.6743
    • Subrota Hati, Shilpa Vij, Surajit Mandal and  R.K. Malik (2014) α-Galactosidase Activity and Oligosaccharides Utilization by Lactobacilli during Fermentation of Soy Milk. Journal of Food Processing and Preservation (Impact Factor: 0.45). 06/2014; 38(3). DOI: 10.1111/jfpp.12063
    • Shilpa Vij,Priyanka Chandra, and Prashant Ramrao Bachanti 2014. Antimicrobial Activity of Casein Fermentate of Probiotic Lactobacillus spp. International Journal of Genetic Engineering and Biotechnology 5, 2, pp. 201-208
    • Hati S, Vij Sand Mandal S. 2014. Effect of Antioxidative activity and polyphenol content of fermented soy milk.  BIOVED24(2) 225-234
    • Brij Pal Singh, Shilpa Vij,Subrota Hati (2014) Review- Functional significance of bioactive peptides derived from soybean Peptides 54 (2014) 171–179.
    • Subrota Hati, Shilpa Vij, Brij Pal Singh and Surajit Mandal (2014) β- glucosidase activity and Bioconversion of isoflavones during fermentation of soymilk. Journal of the Science of Food and Agriculture      DOI: 10.1002/jsfa.6743
    • Vandna, Shilpa Vijand Subrota Hati (2014) Physico-chemical and sensory quality of probiotic fermented whey drinks and its storage study Indian J Dairy Science Vol.67 No.2
    • Subrota H., Shilpa V, Brij S., Vandna K. and Surajit M (2013)  Antioxidative activity and polyphenol content in fermented soy milk supplemented with WPC-70 by probiotic Lactobacilli  International Food Research Journal 20(5): 2125-2131.
    • Subrota Hati, Shilpa Vij, Surajit Mandal, R.K. Malik, Vandna Kumari and Yogesh Khetra (2013)  α-Galactosidase Activity and Oligosaccharides Utilization by Lactobacilli during Fermentation of Soy Milk.   Journal of Food Processing and Preservation37(1): 9   JAN 2013, DOI: 10.1111/jfpp.12063
    • Kairam Narsaiah, Robin A Wilson; Harshad M. Mandge; S. N, Jha; R. K. Malik;Shilpa Vij (2013) “Optimization of encapsulation and controlled release of nanoliposome loaded pediocin in alginate-guar gum- pectin matrix”  Carbohydrate Polymers
    • Shilpa Vij,Shital Borkar, Subrota Hati, Ravinder K Malik, and Gurpreet Kaur (2012) Antibiotics Resistance Pattern of Common Disease-Causing Pathogens. Indian J Dairy Science Vol 65 (1): 47-51
    • Yadav D, Vij S, Hati S, Singh BP, Dhanday M, Dahiya M and Vandna (2012) Evaluation Of Total Antioxidant Activity Of Soy Yoghurt.  Indian J Dairy Science65(3), 220-224
    • Narasaiah K, Jha SN, Wilson RA, Mandge HM, Manikantan MR, Malik RK and Vij S(2012) Pediocin loaded nanoliposomes delivery system for slow release of Pediocin  BioNano Sci. DOI 10.1007/s12668-012-0069-y
    • Minakshi Dahiya, ShilpaVij (2012). Hydrolysis of whey lactose using CTAB permeabilized thermotolerant yeast cells for subsequent fermentation to bioethanol. International J Advanced Biotechnology and Bioinformatics 1 (1), 65-68.
    • Falguni Patra, Shilpa Vijand B. Mann 2010. Production of proteinaceous antifungal substances from Lactobacillus brevis NCDC 02 International J. Dairy Technology Volume 63, 1,70-76.
    • Hati, S., Gandhi, D.N. Vij, S.; Mandal, S.and Dahiya, M. 2010  Process optimization of a drinkable acidophilus milk Indian Journal of Dairy Science.  (5). 350-357
    • Arun Bhardwaj, Hittu Gupta, Suman Kapila, Gurpreet Kaur, Shilpa Vij,Ravinder Kumar Malik 2010. Safety assessment and evaluation of the probiotic potential of bacteriocinogenic Enterococcus faecium KH 24 strain under in vitro and in vivo conditions. International Journal of Food Microbiology .141:156–164,

    Books Authored:

    • Suja Senan, RK Malik &Shilpa Vij (e-Course Book, 2012) Food & Industrial Microbiology

    Book Chapters:

    • Arun Beniwal, Priyanka Saini, Jagrani Minj, Shilpa Vij (2018). Enabling System Biology in Yeast for the Production of Advanced Biofuels in Microbial Cell Factories CRC Press eBook ISBN 9781351668057 p 175-191.
    • Brij Pal Singh and Shilpa Vij 2015. Potential Health Benefits of Fermented Soy Milk. Scientific India, ISSN: 2349-1418, 3(5): 26.
    • Brij Pal Singh, Shilpa Vij, Subrota Hati, Deependra Singh and Bharat Bhushan 2016. Biofunctional Significance of Soybean Based Food Products Dairy and Food Product Technology. ISBN 978-81-7622–358-4, 49-59, BIOTECH BOOKS, Darya Ganj, New Delhi-110002.
    • Deepansh Sharma, Arun Beniwal, Priyanka Saini, Shailly Kapil, Shilpa Vij (2018). Engineering Microbial Cell Factories for Improved Whey Fermentation to Produce Bioethanol. in Microbial Cell Factories CRC Press eBook ISBN 9781351668057 p353-370
    • Hati S, Vij S,Mandal S, Khamrui K. 2014.Utilization of Soy Oligosaccharides during fermentation by Lactobacilli.Book: Oligosaccharides: Food Source, Biological Roles and Health Implications. Nova Science Publishers, Inc., New York, pp. 233-251
    • Jagrani Minj, Priyanka Saini, Shayanti Minj, Arun Beniwal, Deepansh Sharma, Shriya Mehta, Shilpa Vij (2018). Beneficial Effects of Dairy Foods Enriched with Prebiotics and Probiotics. In Microbial Cell Factories CRC Press eBook ISBN 9781351668057 p371-398.
    • Minakshi Dahiya,Shilpa Vij and Subrota Hati (2013)   Biofuels: Current Trends and Future Prospects in” Dairy and Food Processing IndustryRecent Trends— Part I — “Published by Biotech Books New Delhi – 1 p77.
    • Sharma D, Beniwal A Saini P Kapil S, Vij S and Malik RK (2018) Metabolic engineering in yeast for bioethanol production from whey. P-411 in ‘Microbes in Action’. CRC Press.Taylor and Francis, USA.

    Manuals (4 NOS.):

    • K. Malik, S. Vijand N. Kumar 2009 A Laboratory Manual on: “Microbiology of Processed Dairy Foods”. NDRI Publication No.: 44/2009 Total Pages:100
    • Shilpa Vij, K. Malik, and A. K. Puniya2010 Dairy Biotechnology- A laboratory manual NDRI Publication No.: 59/2010 Total Pages:116.
    • Shilpa Vij2008 “Microbial Physiology”- A Laboratory Manual NDRI Publication no.36/2008., Karnal Intech Publications, Karnal
    • AK Puniya and Shilpa Vij Food and Industrial Microbiology. A Laboratory Manual 2010. NDRI Publication no.60/2010. Published by Intech Publications, Karnal

    Patents Filed:

    • Construction of a mutant strain of Kluyveromyces marxianusfor enhanced galactose utilization. (Application Number-201711030808 E-2-1346/2017/DEL).
    • A process for bioethanol production by immobilized stress tolerant microorganism (3710/DEL/2011)

    Lab members:

    Scholars

    1. PhD
    •  Reenu Kashyap

    Thesis title: Buffalo colostrums proteins and peptides derived formulation for diarrhea treatment in immunocompromised mice.

    Period: 2015 onward

    •   Daraksha Iram

    Thesis title: Elucidation and role of milk derived potent antimicrobial bioactive peptides against multi drug resistant bacteria

    Period:  2016 onward

    • Mohamad Rizwan:2017
    • Upma Verma:2017
    •  Chandni:2018
    1. Master
    • Suman S

    Title:Preparation and Evaluation of Bio-functional Fermented Goat Milk using Native Lactic Acid Bacteria

    Period :2018

    •  Priya Ashok

    Microencapsulation of colostrum whey protein derived antimicrobial bioactive peptides (amp) for application in packaging

    Period :2018

    • Ankita Rawat: 2017

     

    Dr. Archana Verma

    Qualifications :

    • M.Sc.
    • Ph.D.

    Major Research Accomplishments:

    • Genetic variation in a number of candidate genes related to milk production & composition(GH, Beta-lactoglobulin, alpha-casein, beta-casein, kappa-casein, leptin, prolactin, ATF4 (Activating Transcription Factor 4),OLR1 (Oxidized low density lipoprotein receptor 1 ),SOCS2 (Suppressor of Cytokine Signalling 2),CRH (Corticotrophin Releasing Hormone); reproduction (BSP5 (Binder of Sperm5), ODF1 (Outer dense fiber of sperm tail 1),PLCZ1 (Phospholipase C zeta 1), ZNF280BY (Zinc finger protein 280BY), PROP1 (Prophet of PIT1), SPAG11, ITGB5, protein phosphtase1 regulatory subunit 11 (PPP1R11), heat shock proteins(HSP27, HSP90AA1, HSPB1, HSF1); mastitis (lactoferrin, Interleukin-8 (IL-8),  Toll-like receptor 4 gene (TLR 4), Forebrain embryonic zinc finger like gene (FEZL), Caspase activating recruitment domain 15 gene (CARD 15), Calgranulin gene (S100A8), Cluster of Differentiation 14 (CD 14) gene, Mannose binding lectin (MBL1, MBL2) C4A); and type traits (GC (GC, vitamin D binding protein), NPFFR2 (neuropeptide FF receptor 2), PRLR (Prolactin receptor), FAM19A1 (family with sequence similarity 19 member A1, C-C motif chemokine like), KCNB1 (potassium voltage-gated channel subfamily B member 1), EDN3 (endothelin 3)have been explored and their association has been established in indigenous and crossbred cattle and Murrah buffalo.
    • Genome-wide SNPs and SSRs have been identified in Gir and Tharparkar cattle.
    • Genome-wide association study with milk production in Murrah buffaloes is in progress.

    Research Projects:

    • Institute: 5 (3 as PI)
    • Externally Funded: 2 (as co-PI)

    Teaching:

    M.V.Sc.:

    • AG-611 (Animal Cytogenetics and Immunogenetics)
    • AG-621 (Molecular Genetics in Animal Breeding)

    Ph.D.:

    • AG-711 (Recent Advances in Animal Genetics)
    • AG-722 (Advances in Molecular Cytogenetics)

    Students Guided:

    • M.Sc./M.V.Sc.: 19 + 3 (presently working)
    • M.Phil./Ph.D.: 14 +1 (presently working)

    Students Guided:

    • M.Sc./M.V.Sc.: 19 + 3 (presently working)
    • M.Phil./Ph.D.: 14 +1 (presently working)

    Transfer of Technology:

    • ‘Detection of buffalo milk in cow milk using Hansa Test Serum’ transferred to M/s Mother Dairy, New Delhi

    Patents, Technology, Methodology, Genetic Stock, Variety, etc.:

    • A total of 123 Gene Bank Accession Numbers have been received for a number of genes in Sahiwal cattle and Murrah buffaloes.

    Publications:

    • Azad M. S., I. D. Gupta, Archana Verma, Raja. K. N, Ritwik Hazra and Rajesh Kumar S 2012. Detection of coagulation factor XI gene deficiency in Sahiwal cattle. The Indian Veterinary Journal. 89 (6):61-62.
    • Datta Sanjoy, Archana Verma, I. D. Gupta, Indrasen Chauhan, Mandeep S. Azad, Yathish H.M., N. K. Batul, and T. Har. 2012. Identification of polymorphism in leptin gene of Murrah buffalo by PCR-RFLP. Exploratory Animal and Medical Research 2 (1) : 7-17
    • Datta Sanjoy, AMJB Adhikari, Anuj Chauhan, Archana Verma, I.D. Gupta, Inderasen Chauhan, Mandeep S. Azad and Yatish H. M. 2012. Nucleotide Sequence Variation in Leptin Gene of Murrah Buffalo (Bubalus bubalis). Exploratory Animal and Medical Research 2 (2): 130-136.
    • Manoj M, Gandhi R.S, Raja T.V, Verma Archna, Singh Avtar and Sachdeva Gk 2012. Growth rates and growth curve in Sahiwal cattle 2012. Indian Journal of Dairy Science 65 (4): 354-355.
    • Raja K. N., Gupta. I. D. and Archana Verma. 2012. Single strand conformation polymorphism at 5’ flanking region of lactoferrin gene in Sahiwal cattle. The Indian Veterinary Journal 89 (9): 16-18.
    • Soumya N. P., D. Gupta, Archana Verma, K. N. Raja, Inderasen Chauhan, Mahesh Chaudhari and R. S .Wakchaure. 2013. Identification of CARD-15 Gene Polymorphism in Sahiwal Cattle. Indian J. Anim. Res. 47 (1): 83-86.
    • Verma Archana, Gupta I D and Gandhi R S. 2012. Genetic polymorphism in exon 3 of prolactin gene in Indian Murrah buffaloes. Wayamba Journal of Animal Science. http://www.wayambajournal.com, DOI 1337018272.
    • Virendra Kumar, D. Gupta, Archana Verma, S. Rajesh Kumar and M.V. Chaudhari. 2013. CD14 Gene Polymorphism using HinfI RE and its Association with Mastitis in Sahiwal Cattle. India J. Anim. Res. (Accepted vide No. ARCC/B-2535).
    • Wakchaure R.S., I. D. Gupta, Archana Verma, Dinesh Kumar and Rajesh Kumar S. 2012. PCR-RFLP with Alu I RE enzyme in TLR4 gene of Sahiwal Cattle. Indian J. Anim. Res. 46 (2): 205-207.
    • Wakchaure R. S., I. D. Gupta, Archana Verma, Dinesh Kumar and G. S. Sonwane. 2012. Association of Genetic Variants of Partial Exon 3 Region of TLR4Gene with Mastitis in Sahiwal Cattle”. Indian J. Anim. Res. 46 (2): 208-209.
    • Archana Verma (2013) Nucleotide sequence variability in exon 4 of prolactin locus in Murrah buffaloes (Bubalus bubalis). Research Journal of Biotechnology 8 (6):61-65.
    • Sanjoy Datta, Archana Verma, Paresh Chatterjee and Aruna Pal Chatterjee (2013) Molecular characterization of the leptin gene in riverine buffaloes. Buffalo Bulletin (Thailand). 32(3): 196-211.
    • Alka Chopra, I. D. Gupta, Archana Verma, Soumya N. P. and V. Vohra (2013) Identification of Lactoferrin gene Polymorphism and its association with Mastitis incidence. Journal of Animal Research, 3 (1): 103-108.
    • Wakchaure, R. S., I. D. Gupta, Archana Verma, Dinesh Kumar and Rajesh Kumar S. (2013) PCR-RFLP with Alu I Restriction Enzyme in TLR4 Gene of Sahiwal Cattle. Indian J. Anim. Res. 47 (2): 172-174.
    • Indrasen Chauhan, Archana Verma, Ishwar Dayal Gupta and Gokul S. Sonawane (2013) Genetic Polymorphism in Caspase Activating Recruitment Domain 15 and its Association with Incidence of Mastitis in Murrah Buffalo. Buffalo Bulletin, 32 (Special Issue 2): 723-728.
    • Archana Verma, Ishwar Dayal Gupta and Ravinder Singh Gandhi (2013) Genetic Variability in Production and Immune Function Genes Associated with Production Traits and Incidence of Mastitis in Indian Murrah Buffalo. Buffalo Bulletin, 32 (Special Issue 2): 729.
    • Alka Chopra, I. D. Gupta, Archana Verma and V. Vohra (2014). PCR-RFLP analysis of bovine lactoferrin promoter and its association with mastitis incidence in crossbred (Karan Fries) cattle. The Indian J. Vet. Sci. 2(1): 34-38.
    • P. Soumya, I. D. Gupta, Archana Verma, K. N. Raja, Inderasen Chauhan, Mahesh Chaudhari and R. S .Wakchaure. (2013). Identification of CARD-15 Gene Polymorphism in Sahiwal Cattle. Indian J. Anim. Res. 47 (1): 83-86.
    • Alka Chopra, I. D. Gupta, Archana Verma and V. Vohra (2014). Detection of Allelic Variants in Lactoferrin gene promoter using Created Restriction Site PCR-RFLP and its Association with Mastitis. The Indian Journal of Animal Sciences 84 (10): 1068-1070.
    • Raja K. N., Gupta. I. D. and Archana Verma (2014). Sequence characterization of lactoferrin gene promoter region in Bos indicus cattle. Indian J. Anim. Res. 48 (1): 6-10.
    • Virendra Kumar, I. D. Gupta, Archana Verma, S. Rajesh Kumar and M. V. Chaudhari (2014). CD14 gene polymorphism using Hinf restriction enzyme and its association with mastitis in Sahiwal cattle. Indian J. Anim. Res., 48 (1): 11-13.
    • Sakthivel Selvan, I. D. Gupta, Archana Verma, Mahesh V. Chaudhari, Shishir Kanungo and S. Rajesh Kumar (2014). Characterization of promoter region of CD14 gene and Association with clinical mastitis in Karan Fries cattle. Indian J. Anim. Res.,48 (6): 545-547.
    • Selvan A.S., Gupta I.D., Verma A., Chaudhari M.V., Kumar V. (2014) Cluster of differentiation 14 gene polymorphism and its association with incidence of clinical mastitis in Karan Fries cattle. Veterinary World 7(12): 1037-1040.
    • Arya Ashwani, Archana Verma, I. D. Gupta, Shahid A. Shergojry, Ankit Magotra, Dhaman Kumar and Arun P. Singh (2015) Molecular characterization and SNP identification in HSPB1 gene in Murrah buffalo. Indian Journal of Animal Research. 48 Online Published Date: 2015-03-10 DOI: 10.5958/0976-0555.2015.00035.7
    • Chakraborty Dibyendu, Singh Avtar, Tantia MS, Verma Archana and Chakravarty AK. 2015. Genetic polymorphism of BOLA-DRB 3.2 locus in Sahiwal cattle.Animal Science Reporter. 9(1): 33-40.
    • Chopra Alka, I. D. Gupta, Archana Verma and V. Vohra (2014). Detection of Allelic Variants in Lactoferrin gene promoter using Created Restriction Site PCR-RFLP and its Association with Mastitis. The Indian Journal of Animal Sciences 84 (10): 1068-1070
    • Chopra Alka, I. D. Gupta, Archana Verma and V. Vohra (2014). PCR-RFLP analysis of bovine lactoferrin promoter and its association with mastitis incidence in crossbred (Karan Fries) cattle. The Indian Journal of Vet. Sci.  2(1): 34-38. ijmanager.org/ index.php/ijvs
    • Das Ramendra, I. D. Gupta, Archana Verma, Avtar Singh, Mahesh V Chaudhari, Lalrengpuii Sailo, R. C. Upadhyay, J. Goswami (2015). Genetic polymorphisms in ATP1A1 gene and their association with heat tolerance in Jersey crossbred cows. Indian J. Dairy Sci. 68(1): 50-54.
    • Lalrengpuii Sailo, I. D. Gupta, Archana Verma, Avtar Singh, Mahesh Vishwas Chaudhari, Ramendra Das, R.C. Upadhyay, Goswami (2015). Single nucleotide polymorphism in HSP90AB1 gene and its association with thermo-tolerance in Jersey crossbred cows. Animal Science Reporter 9(2):43-49
    • Manoj M, Gandhi R S, Raja TV, Verma Archana, Singh Avtar, Sachdeva GK and Amit Kumar. 2014. Genetic Parameters of body weights at different ages in Sahiwal Heifers. Indian Journal of Animal Research. 48 (3): 217-220.
    • Selvan A.S., Gupta I. D., Verma A., Chaudhari M. V., Kumar V. (2014) Cluster of differentiation 14 gene polymorphism and its association with incidence of clinical mastitis in Karan Fries cattle, Veterinary World 7(12): 1037-1040.
    • Selvan Sakthivel, Gupta I.D., Archana Verma, Mahesh V. Chaudhari, Shish Kanungo and S. Rajesh Kumar (2014). Characterization of promoter region of CD14 gene and Association with clinical mastitis in Karan Fries cattle. Indian J. Anim. Res.,48 (6): 545-547.
    • Krishanender Dinesh, Archana Verma, I. D. Gupta, Y. P. Thakur, NishantVerma and AshwaniArya (2015). Identification of polymorphism in exons 7 and 12 of lactoferrin gene and its association with incidence of clinical mastitis in Murrah buffalo. Tropical Animal Health and Production 47: 643-647.
    • Ashwani Arya, Archana Verma, I D Gupta, Shahid A Shergojry, Ankit Magotra, Dhaman Kumar and Arun P Singh (2016)  Molecular Characterization and SNP Identification in HSPB1 Gene in Murrah Buffalo. Indian Journal of Animal Research 50 (4): 645-649
    • Ashwani Arya, Archana Verma, I D Gupta, Dhaman Kumar, Ankit Magotra, Mohsin A. Mir and Arun P Singh (2016) Influence of parity, THI and HSPB1 gene SNP on heat tolerance indicator traits in Murrah buffalo (Bubalus bubalis). Ruminant Science 5 (2): 143-148.
    • Lalrengpuii Sailo, I D Gupta, Archana Verma, Ramendra Das, M V Chaudhari, Sohanvir Singh(2016) Polymorphisms in HSP90AB1 gene and their association with heat tolerance in Sahiwal and Karan Fries cows. Indian Journal of Animal Research 50 (6): 856-861.
    • NishantVerma, IshwarDayal Gupta, Archana Verma, Rakesh Kumar, Ramendra Das and Vineeth M R (2016)Novel SNPs in HSPB8 Gene and their Association with Heat Tolerance Traits in Sahiwal Indigenous Cattle. Tropical Animal Health and Production 48(1): 175-18.
    • Rakesh Kumar, Ishwar Dayal Gupta, Archana Verma,Sohan Vir Singh, Nishant Verma, M R Vineeth, Ankit Magotra and Ramendra Das (2016) Novel SNP Identification in Exon 3 of Hsp90AA1 Gene and their Association with Heat Tolerance Traits in Karan Fries (Bos Taurus × Bos indicus) Cows under Tropical Climatic Condition. Tropical Animal Health and Production. 48 (4): 735-740
    • Rakesh Kumar, Ishwar Dayal Gupta, Archana Verma,  Nishant Verma and  Vineeth M.R. (2017) Single nucleotide polymorphisms in Heat Shock Protein HSP90AA1 gene and their association with heat tolerance traits in Sahiwal cows. Indian J Animal Research 51 (1): 64-69.
    • Ramendra Das,  IshwarDayal Gupta, Archana Verma,  Sohanvir Singh, Mahesh Vishwas Chaudhari ,  Lalrengpuii Sailo,  Nishant Verma and Rakesh Kumar (2017) Single nucleotide polymorphisms in ATP1A1 gene and their association with thermo-tolerance traits in Sahiwal and Karan Fries cattle. Indian Journal of Animal Research 51 (1): 70-74.
    • Sourabh Sulabh, Archana Verma and D. Gupta (2016) Genotyping of Calgranulin A Gene in Murrah Buffaloes by PCR-RFLP. Indian Journal of Dairy Science. 69 (2): 178-181.
    • Sourabh Sulabh, Archana Verma, I D Gupta and S Rajesh Kumar  (2016) Detection of Polymorphism of Calgranulin A Gene in Indian Murrah Buffalo. Indian Journal of Animal Research: 50 (5): 658-661.
    • Sourabh Sulabh, Archana Verma, I D Gupta and Bharat Kumar (2018) Novel SNPs in DGAT1 gene and their association with milk traits in Murrah buffaloes. Indian J Dairy Sci 71(2):187-192.

    Dr. Vikas Vohra

    Qualifications:

    • Ph.D. in Animal Genetics and Breeding from National Dairy Research Institute, Karnal
    • M.V.Sc. in Animal Genetics and Breeding from Indian Veterinary Research Insitute, Izatnagar, Bareilly
    • B.V.Sc. & A.H. from College of Veterinary and Animal Sciences, Mathura

    Major Research Accomplishments:

    • Concept of enumeration of grades within the major livestock breeds was proposedto DAHDAF, Government of India, which was implemented during National Breed Survey, 2013. Same concept is being adopted for the livestock enumeration in the forthcoming 20th Livestock Survey, 2017.
    • Surveyed, identified, characterized and documented the lesser known animal genetic resource of India, which includes Belahi Cattle, Gojri Buffalo, Chhattisgarhi Buffalo, Bargur Buffalo and Parlekhimundi, Sambalpuri, Kalahandi, Manda buffalo of Odisha; Kajali and Poonchi Sheep; Bakarwali and Rohailkhandi Goat populations.
    • Proof of concept that neutral microsatellite markers and their allele frequency, FST values can be applied in classifying nondescript livestock in to genetically distinct breeds. Following this concept diversity of lesser known germplasm was established.
    • Candidate gene approach was used in characterization of genes related to milk fat, growth and reproduction were studied. The work has enriched the bovine and ovine genome database and these gene variants generate new opportunities to be used in Marker Assisted Selection.
    • Developed a methodology to identify the persistent dairy cows using leptin genotype profile for maximizing the lifetime milk production.

    Guidance to Student:

    • M.V.Sc. = 3
    • PhD = 2

    Books Authored:

    • Sustainable Breeding in Cattle and Buffalo. 2011. ISBN 81-89304-98-4

    Books Edited:

    • Conservation of Indigenous Domestic Animal Diversity. 2016. ISBN 978-93-83537-29-7
    • Management of Animal Genetic Resources in India. 2015. ISBN 978-93-83537-23-5

    Reports Prepared:

    BOOK CHAPTER /MANUAL/ MONOGRAPH:

    • Book Chapters Written = 6
    • Teaching Manual written = 4
    • Monographs written = 6
    • Training compendium = 3

    Editorship of NAAS rated journal

    • Founder and Associate editor, Journal of Animal Research, a Bimonthly peer reviewed research publication in Animal Science. (NAAS rating 5.68, 2018)
    • Advisory Board Memberof NAAS rated journal, Journal of Livestock Biodiversity, a half yearly publication by SOCDAB Society (NAAS rating 2.38, 2018)

    Awards:

    • Associate ship Award from National Dairy Science Academy-India
    • Breed Saviour Award from National Biodiversity Authority of India & SEVA & Life Network
    • Young Scientist Award received from Indian Association for the Advancement of Animal Research, Bareilly
    • University Topper (Ph.D.) from ICAR-NDRI (Deemed University) – Karnal
    • B.V.Sc. & A.H. Merit Scholar Award from College of Veterinary and Animal Sciences – Mathura (U.P.)
    • Junior Research Fellowship for M.V.Sc. from Indian Council for Agricultural Research, New Delhi
    • Junior Research Fellowship from Indian Council for Medical Research, New Delhi
    • Received Outstanding Achievement Award, for meritorious and significant contribution made in Agriculture and Allied Sciences from Society for Bioinformatics and Biological Sciences
    • Prof. KN Sharma, Memorial Award for Best Research Paper presented by SOCDAB
    • Best Oral Presentation Award (3)
    • Best Poster Award (4)
    • Best Stall of the Institute during Exhibition, Kisaan Mela, etc. (4)

    Training:

    • Databases and Software for Analysis of Animal Genetic and Breeding Data
    • Advances in Breeding Management Technologies for the Improvement in Livestock Productivity
    • Genetic Improvement of Farm Animals Using Advanced Breeding and Biotechnological Strategies

    Patents, Technology, Methodology, Genetic Stock, Variety, etc.:

    • REGISTERED THREE NEW BREEDS OF LIVESTOCK AT NATIONAL LEVEL.
    • 1. Belahi Cattle – Identified, Characterised and Registered as 38th Cattle Breed of India. Accession No. INDIA_CATTLE_0532_BELAHI_03038.
    • 2. Chhattisgarhi Buffalo – Identified, Characterised and Registered as 16th Buffalo Breed of India. Accession No. INDIA_BUFFALO_2600_CHHATTISGARHI_01016
    • 3. Bhakarwali Goat – Identified, Characterised and Registered as 34th Goat Breed of India. Accession No. INDIA_GOAT_0700_BHAKARWALI_06034
    • Thirty four (34) gene Sequences submitted in National Centre of Biotechnology gene bank (USA) and Accession No(s) were obtained.

    Publications:

    • Vohra V, Bhattacharya T K, Dayal S, Kumar P and Sharma A. 2006. Genetic variance of Beta lactoglobulin gene and its association with milk composition traits in riverine buffalo. Journal of Dairy Research 73: 499-503
    • Bhattacharya T K, Mishra S S, Sheikh F D, Dayal S, Vohra V, Kumar P and Sharma A. 2004. Variability of milk fat globule membrane protein gene between cattle and riverine buffalo. DNA Sequence- The Journal of Sequencing and Mapping 15 (5-6): 326-331
    • Dayal S, Bhattacharya T K, Vohra V, Kumar P and Sharma A. 2006. Effect of alpha lactalbumin gene polymorphism on milk production traits in water buffalo Asian Australian Journal of Animal Sciences 19(3): 305-308
    • Dayal S, Bhattacharya T K, Vohra V, Kumar P and Sharma A. 2006. Genetic polymorphism of alpha lactalbumin gene in riverine buffalo DNA Sequence-The Journal of Sequencing and Mapping 16(3): 173-179
    • Sharma N, Kang TY, Lee S J, Jin Nam Kim, Chang Hyun Hur, Jong Chul Ha, Vohra V, Dong Kee Jeong. 2013. Status of bovine mastitis and associated risk factors in subtropical Jeju Island, South Korea. Tropical Animal Health and production. 45: 1829-1832
    • Vohra V, Niranjan S K, Mishra A K and Joshi B K. 2014. Belahi Cattle Pastoralism in the Himalayan Foot Hills. Proc. Aust. Soc. Anim. Prod. 2014 Vol. 30: 317
    • Chandershekhar S Patil, Chakravarty A K, Singh A, Kumar V, Jamuna V and Vohra V. 2014. Development of a predictive model for daughter pregnancy rate and standardization of voluntary waiting period in Murrah buffalo. Tropical Animal Health and production. 46: 279–284
    • Jamuna V, Chakravarty A K, Patil C S, Dash S K, Mahajan A C, Kumar V and Vohra V. 2014. Enhancing milk and fertility performances using selection index developed for Indian Murrah buffaloes. Tropical Animal Health and production 46: 967–974.
    • Vohra V, Niranjan S K, Mishra A K, Jamuna V, Chopra A, Sharma N and Dong Kee Jeong. 2015. Phenotypic characterization and multivariate analysis to explain body conformation in lesser known buffalo (Bubalus bubalis) from North India. Asian Australian Journal of Animal Sciences 28(3): 311-317.
    • Chopra A, Gupta I D, Verma A, Chakravarty A K and Vohra V. 2015. Lactoferrin gene promoter variants and their association with clinical and sub clinical mastitis in indigenous and crossbred cattle. Polish Journal of Veterinary Sciences 18(3): 465-471
    • Niranjan S. K, Goyal S, Dubey P. K, Vohra V, Singh S, Kathiravan P and Kataria R. S. 2016. Molecular Characterization of Buffalo Haptoglobin: Sequence based structural comparison indicates convergent evolution between ruminants and human. Animal Biotechnology, 27(1):30–37
    • Jamuna V, Chakravarty A K, Kumar V, Mir MA and Vohra V. 2016. Standardizing Pregnancy Rate of Indian Murrah Buffaloes for Higher Milk Yield. Buffalo Bulletin, 35(1):109-119
    • Kumar M, Vohra V, Ratwan P and Chakravarty AK. 2016. SNP identification in thioesterase domain of fatty acid synthase gene in Murrah buffaloes. The Journal of Animal and Plant Science. 26(6): 1828-1832
    • Vohra V, Sodhi M, Niranjan S K, Mishra A K, Chopra A, Kumar M and Joshi A K. 2017. Characterization of rare migratory cattle and evaluation of its phylogeny using short-tandem-repeat-based markers. Journal of Applied Animal Research, 45(1): 355-363
    • Mishra S K, Dubey P K, Dhiman A, Dubey S, Verma D, Kaushik AC, Singh R, Niranjan SK, Vohra V, Mehrara KL, and Kataria RS (2019) Sequence-based structural analysis and evaluation of polymorphism in buffalo Nod-like receptor-1 gene. 3 Biotech (2019) 9(1): 26 https://doi.org/10.1007/s13205-018-1534-2
    • Sharma R, Ahlawat S, Sharma H, Kulkarni VS, Kataria RS, Vohra V and Tantia MS. 2019. Microsatellite based Genetic Diversity and Mutation-Drift Equilibrium in Dharwadi Buffalo (Bubalus bubalis) of India. Agric. Res. published online https://doi.org/10.1007/s40003-018-0396-5
    • Mishra SK, Dubey PK, Goyal S, Singh S, Niranjan SK, Vohra V, Mukesh M and Kataria RS. 2019. Identification of novel polymorphism in buffalo stanniocalcin-1 gene and its expression analysis in mammary gland under different stages of lactation. Journal of Genetics (2019) 98:38 https://doi.org/10.1007/s12041-019-1082-5
    • Vohra V, Chakravarty A K and Chopra A. 2010. Revealing Different Shapes of Lactation Curves in Karan Fries Cows. Indian Journal of Dairy Science 63(6): 459-63
    • Vohra V, Niranjan S K, Singh P K, Sadana D K and Joshi B K. 2011. Pastoral Management of Kankrej Cattle during Migration between Western Dryland and Trans-Gangetic Plain of India. Indian Journal of Dairy Science 64(6): 501-507
    • Vohra V, Chakravarty A K and Chopra A. 2011. Lactation Curve modeling in Karan Fries Cows using empirical mathematical model. Journal of Animal Research 1(1): 57-61
    • Vohra V, Chakravarty A K, Singh A, Gupta I D, Chopra A, Dubey P P and Kumar D. 2011. Identification of leptin gene polymorphism and its association with 305 day milk yield in Karan Fries cows. Indian Journal of Animal Science. 81(4): 388-390
    • Vohra V, Dayal S and Bhattacharya T K. 2012. SSCP typing of alpha-lactalbumin and beta-lactoglobulin gene and its association with milk production and constituent traits in Indian riverine buffalo. Indian Journal of Animal Science 82(8): 884-888
    • Nawle V S, Chakravarty A K, Chakraborty D, Vohra V. 2012. Effects of non-genetic factors on different reproductive traits in Murrah buffalo. Indian Veterinary Journal 89(12): 18-19
    • Vohra V, Niranjan S K and Joshi B K. 2012. Gojri– A novel migratory buffalo germplasm in Punjab and Himachal Pradesh. Journal of Animal Research 2(3): 17-321
    • Vohra V, Niranjan S K and Joshi B K. 2012. Belahi Cattle: Uniform but distinct germplasm of Haryana. Journal of Animal Research 2(1): 47-51
    • Chopra A, Gupta I D, Verma A, Soumya N P and Vikas V. 2013. Identification of lactoferrin gene polymorphism and its association with Mastitis Incidence. Journal of Animal Research 3(1): 103-108
    • Chopra A, Gupta I D, Verma A and Vohra V. 2014. Detection of Allelic Variants in Lactoferrin gene promoter using Created Restriction Site PCR-RFLP and its Association with Mastitis. Indian Journal of Animal Sciences 89(10): 36-38
    • Jamuna V, Chakravarty A K, Patil C S, Mahajan A C, Dash S and Vohra V. 2013. Decline in Reproductive performance in High producing Murrah Buffalo. Journal of Animal Research 3(2): 203-208
    • Vohra V, Kumar M, Chopra A, Niranjan S K, Mishra A K and Kataria R S. 2015. Polymorphism in exon-40 of FASN gene in lesser known buffalo breeds of India. Journal of Animal Research 5(2): 325-328
    • Bukhari S, Das A K, Kumar N, Raghuvanshi P, Taggar R K, Chakraborty D, Kumar D, Vohra V and Gupta P. 2015. Genetic polymorphism of promoter region of lactoferrin gene and its association with mastitis resistance in Jersey crossbred cattle. Indian Journal of Animal Research 49(2): 165-167
    • Yatoo SA, Taggar RK, Chakraborty D, Kumar N, Kumar D and Vohra V. 2015. Status of FecB gene mutation in Dorper Sheep from Jammu. Journal of Animal Research 5(4): 253-55
    • Kumar M, Vohra V, Ratwan P, Chopra A and Chakravarty A K. 2017. Influence of FASN gene polymorphism on milk production and its composition traits in Murrah buffaloes. Indian Journal of Animal Research 51 (4): 640-643
    • Kumar M, Vohra V, Ratwan P and Chakravarty A K. 2015. Exploring polymorphism in 3′ UTR region of STAT1 gene in different Buffalo breeds. Indian Journal of Dairy Science 68(5): 473-476
    • Jamuna V, Gupta A K, Singh A, Patil C S, Kumar M and Vohra V. 2016. Leptin gene polymorphism in association with lactation milk yield in Murrah buffaloes. Indian Journal of Animal Science 86(1): 95-97
    • Kumar M, Vohra V, Ratwan P, Valsalan J, Patil CS, Chakravarty AK. 2016 Estimates of genetic parameters for fat yield in Murrah buffaloes, Veterinary World, 9(3): 295-298
    • Vohra V, Mishra AK, Niranjan SK, Chopra A, Kumar M and Joshi BK. 2016. Phenotypic characterization, management and performance of Belahi cattle. Indian Journal of Animal Science. 86(3): 355-358
    • Mishra SK, Mishra AK, Raja KN, Vohra V, Singh S, Singh Y, Ahlawat S and Kataria RS. 2018. Polymorphism analysis at FecB locus in Kajali sheep of India. Indian Journal of Animal Research. 52(3): 474-476
    • Mishra AK, Raja KN, Vohra V, Singh S and Singh Y. 2016. Phenotypic traits and performance of Kajali sheep: a lesser known ovine genetic resource of Punjab, India. Indian Journal of Animal Science. 86(11):1279-1282
    • Mishra AK, Vohra V, Raja KN, Singh S and Singh Y. 2017. Principal component analysis of biometric traits to explain body conformation in Kajali sheep of Punjab, India. Indian Journal of Animal Science. 87(1): 93-98
    • Vohra V, Singh M, Mukherjee K and Kataria R S. 2017. Identification and characterization of Chhattisgarhi buffalo population in India. Indian Journal of Animal Science. 87(2): 182-185
    • Vohra V, Chopra A, and Chakravarty AK. 2017. Prediction of lactation persistency in crossbred cattle using genotype profile of lactation curve traits. Indian Journal of Animal Science. 87(1): 97-104
    • Vohra V, Chopra A, and Chakravarty AK. 2017. Prediction of lactation persistency in crossbred cattle using genotype profile of lactation curve traits. Indian Journal of Animal Science. 87(1): 97-104
    • Vohra V, Singh M, Das R, Chopra A and Kataria R S. 2017. Multivariate analysis of biometric traits and their shared variance in Chhattisgarhi buffalo. Indian Journal of Animal Sciences 87 (7): 864–870
    • Singh S, Mishra AK, Vohra V, Raja KN, Singh Y, Singh KM, Ganguly I And Arora R. 2017. Microsatellite based genetic diversity estimation in Kajali sheep and its phylogenetic relationship with other indigenous sheep breeds. Indian Journal of Animal Sciences 87 (9): 1097–1101
    • Singh R, Niranjan SK, Rajesh C, Mishra SK, Vohra V, Dash SK, Misra D and Kataria RS. 2018. Cytogenetic characterization of Kalahandi and Paralakhemundi buffaloes of Odisha state confirms their riverine status. Indian Journal of Dairy Science (2018) 71(3): 279-283
    • Singh R, Rajesh C, Mishra SK, Gurao A, Vohra V, Niranjan SK and Kataria RS. 2018. Comparative expression profiling of heat-stress tolerance associated HSP60 and GLUT-1 genes in Indian buffaloes. Indian Journal of Dairy Science 71(2):183-186
    • Singh R, Lavakumar S, Gurao A, Mishra SK, Niranjan SK, Vohra V, Dash SK and Kataria RS. 2018. STR Marker based genetic diversity evaluation of Chilika buffalo of Odisha State. Journal of livestock biodiversity 8(1): 29-35
    • Vohra V. 2018. Genomic selection and its significance in Indian dairying. Indian Journal of Dairy Science 71(6): 539-545
    • Lavakumar S, Singh R, Niranjan SK, Mishra SK, Kumar P, Vohra V, Dash SK and Kataria RS. (2019). Cytogenetic characterization of Sambalpuri and Manda buffaloes of Odisha. Indian Journal of Animal Sciences 89 (1): 53–56

    Dr. Chander Datt

    Qualifications:

    • M Sc. (Animal Nutrition)
    • Ph. D. (Animal Nutrition)

    Students guided (As major advisor):

    S. No. Name of student Title of thesis Year of completion
    Mv. Sc.
    1. Ajay Kumar Efficacy of pentasulphate mixture and arsenic in alleviating chronic selenosis and expression of glutathione peroxidase genes in buffaloes 2011
    2 Dinesh Kumar Effect of different dietary protein levels on   nutrient utilization and blood parameters in Sahiwal calves 2012
    3 Ph. Suraj Sharma Variations in metabolism of essential minerals in relation to residual feed intake in growing male Sahiwal calves 2013
    4 Bhong Nana Baban Assessment of residual feed intake and related biochemical parameters in growing male Sahiwal calves 2013
    5 Kuldeep Dudi Studies on nutrient utilization and blood parameters in divergently selected    Sahiwal female calves for residual feed intake 2014
    6 Elsadig Idris Eissa

     

    To study variability in residual feed intake, nutrient utilisation and blood parameters in     Murrah buffalo calves 2015
    7 Digvijay Singh Effect of vanadium supplementation on growth, nutrient            utilisation and blood   biochemical parameters in Sahiwal calves 2016
    8 Khushboo Jain Effect of inclusion of certain micronutrients in the diet on nutrient utilization, immunomodulation and hormonal profile in calves during different environmental conditions 2017
    9 Avinesh Sharma Effect of red seaweed  (Kappaphycus alverazii ) based feed additive on production and health of lactating crossbred cattle. 2018
    10 Thamizhan, P. Contd
    Ph. D.
    1. Akash Mishra Effects of supplementation of nano zinc oxide on zinc

    bioavailability, immunomodulation, expression of zinc responsive genes and performance in buffalo calves

    2017
    2. Swati Shivani Effect of nano copper supplementation on its bioavailability, immunomodulation    and      growth performance in goats Contd.
    3. Shambhvi Effect of Nickel supplementation on nutrient utilization, growth performance and biochemical parameters in calves Contd.

     Research projects handled:

    S.

    No

    Project Duration Funding agency Status of the project

     

    1 Nutritional Evaluation of Local Feed Resources For Ruminants In Tripura (As PI) 2000-07 ICAR Completed
    2 Essential Mineral Status Of Soils, Feeds/Fodders And Animals In Tripura (As PI) 2000-07 ICAR Completed
    3 Effect Of Inclusion Of Local Feed Resources And Probiotics In The Diets Of Non-Ruminants On Growth And Feed Efficiency (As PI) 2006-09 ICAR Completed
    4 Augmentation Of Productive And Reproductive Efficiency In Goat Through Managemental And Hormonal Interventions (As CO-PI) 2007-12

    (Involvement till Jan, 2009)

    ICAR Completed
    5 Genetic Improvement In Growth And Production Traits Of Japanese Quail In Agroclimatic Conditions Of Tripura (As CO-PI) 2007-12

    (Involvement till Jan, 2009)

    ICAR Completed
    6 Livelihood Improvement And Empowerment Of Rural Poor Through Sustainable Farming Systems In Northeast India 2007-

    (Involvement till Jan, 2009)

    NAIP
    7 To Study The Efficacy Of Pentasulphate And Arsenic in Alleviating Chronic Selenosis And Gene Expression of Selenoproteins in Buffaloes (As PI)

     

    March, 2009-Feb.2012 ICAR Completed
    8 A Pilot Study on Development of Organic Milk Production System (As CO-PI)

     

    2008-2011

     

    ICAR Completed
    9 Yield And Quality of Sorghum, Cowpea, Oat And Berseem Fodder Under Organic Manure Based Production System (As CO-PI) 2007-2011)

    Involvement since July, 2009

    ICAR Completed
    10 Network Project On R&D Support For Process Upgradation Of Indigenous Milk Products for Industrial Application-Contamination Component (As CO-PI) 2004-2013

    (Involvement since Aug., 2009)

    Network Project (ICAR) Completed

     

     

     

    11 Participatory Approach On The Effect of Various Micronutrients On Productive Performance Of Dairy Animals Under Field Conditions (CO-PI) March, 2011-Feb-2013 ICAR Completed
    12 Indicators Of Retained Foetal Membrane and Evaluation Of Existing Preventive and Therapeutic Regimes For Maintaining Postpartum Fertility In Dairy Animals (As CO-PI) Since March, 2012-2016 ICAR Completed

     

    13 Assessment Of Variation In Residual Feed Intake in Ruminants (As PI) Since March, 2012-2016 ICAR Completed

     

    14 Evaluating The Impact of Ration Balancing On Methane         Emission In Dairy Animals  (NDDB-NDRI Consultancy Project) As CO-PI May2013-March2015 World Bank Completed

     

    15 NICRA Since 2011 Ongoing
    16 “Confirmation of lactation performance and animal safety of dairy animals of the Bos sp. treated with recombinant bovine somatotropin” with ELANCO Since June, 2013-2014 ELANCO Completed
    17 Precise Energy and protein provisions for transition buffaloes for optimizing production, immunity and health (As CO-PI) April, 2013-March,2016 ICAR Completed
    18 Kappaphycus alvarezii and red seaweed based formulations for improving productivity and health of dairy and poultry animals

    Sub component: To study the effect of Kappaphygus alvarezii based feed additives on production and health of dairy animals (As PI)

     

    April,  2016-March, 2019 CSIR, New Delhi Completed
    19 Studies on vanadium and boron for their role in immune endocrine functions, bioavailabilty of minerals and production performance in dairy animals (As CO-PI) April, 2015-March, 2019 ICAR Completed
    20 Role of certain trace minerals in semen quality of cattle and buffaloes (As CO-PI) April, 2016-March, 2019 ICAR Completed
    21 Salinity and drought stress adaptability of cactus (Opuntia ficus-indica)          as             potential forage in arid environments (Funded by DST, Ministry of Sci. &          Technol.,         GOI): Lead centre- CSSRI, Karnal

    As CO-PI

    April, 2018 DST, Ministry of Sci. &          Technol.,             GOI)

     

    On-going
    22 Studies on iodine deficiency in livestock and its management.   Lead centre- SKAUST, Jammu

    (As CO-PI)

    April, 2018 DST, Ministry of Sci. &          Technol.,             GOI)

     

    On-going

    List of Research Publications:

    S. No Names of author (s) Year Title Journal Vol.: page No. NASS rating
    1. Chander Datt and Singh, G. P 1995 Effect of protein supplementation on in vitro dry matter digestibility and gas production of wheat straw Indian J. Dairy Sci. 48: 357

     

    5.26
    2. Chander Datt and Singh, G. P.. 1995 In vitro digestibility and gas production as affected by supplementation of protein to urea treated wheat straw Indian J. Anim. Nutr. 12: 151-156

     

    5.02
    3.

     

    Chander Datt and Singh, G. P.

     

    1996 Effect of supplementation of protein to untreated and urea treated wheat straw on dry matter intake and nutrient utilization in crossbred cattle Indian J. Anim. Nutr. 13:137-141

     

    5.02
    4. Niranjan, M., Chander Datt and Anubrata Das 2001 Semen quality studies in two White Leghorn lines maintained at Tripura Indian J. Poult. Sci.

     

    36: 287-289 4.35
    5. Chander Datt and Aruna Chhabra 2004 Blood selenium level and glutathione proxidase activity as affected by high level of selenium supplementation in cattle and buffaloes: A comparative study  Indian J. Anim. Sci. 74: 872-877

     

    6.28
    6. Chander Datt, Aruna Chhabra, Niranjan, M., Chattopadhyaya, K., Dhiman, K.R. and Singh, N.P. 2004 In vitro digestibility of straw of different cultivars of paddy

     

    Indian J. Anim. Nutr. 21:149-153 5.02
    7. Chander Datt and Aruna Chhabra 2006 Effect of selenium supplementation on feed intake and nutrient utilization in cattle and buffaloes Indian J. Anim. Sci. 76: 955-960

     

    6.28
    8. Chander Datt, Niranjan, M., Aruna Chhabra and Dhiman, K.R. 2006 Nutritional evaluation of different bamboo leaves of Tripura Indian J. Dairy Sci. 59: 376-379

     

    5.26
    9. Chander Datt, Niranjan, M., Aruna Chhabra, Chattopadhyaya, K. and  Dhiman, K.R. 2006 Forage yield, chemical composition and in vitro digestibility of various cultivars of maize (Zea mays L.)

     

    Indian J. Dairy Sci. 59: 177-180 5.26
    10 Chander Datt, Aruna Chhabra, Bujarbaruah, K.M., Dhiman, K.R. and Singh, N.P. 2007 Nutritional evaluation of leaves of trees and shrubs as fodder for ruminants in Tripura Indian J. Dairy Sci. 60:184-190

     

    5.26
    11 Chander Datt, Aruna Chhabra, Bujarbaruah, K.M., Singh, N. P. and Dhiman, K.R. 2007 Nutritional evaluation of weeds commonly used as fodder for ruminants in Tripura Indian J. Anim. Nutr. 24: 1-6

     

    5.02
    12 Chander Datt, Datta M. and Singh, N.P. 2008 Assessment of fodder quality of leaves of multipurpose trees in subtropical humid climate of India Journal Forestry Res. 19: 209-214

     

    NA
    13 Chander Datt, Aruna Chhabra, Singh, N.P., Bujarbaruah, K.M. 2008 Nutritional characteristics of horticultural crop residues as ruminant feeds in Tripura. Indian J. Anim. Sci. 78: 312-316

     

    6.28
    14 Chander Datt and Aruna Chhabra 2008 Effect of inorganic or organic selenium supplementation on nutrient intake and rumen microbial population and protein content in cattle and buffaloes Indian J. Anim. Sci. 78: 758-763

     

    6.28
    15 Chander Datt, Sankaran, M., Singh, N.P. and Santhosh, B. 2007 In vitro evaluation of cultivars of tapioca and sweet potato tubers for livestock Indian J. Anim. Nutr. 24: 104-10.

     

    5.02
    16 M. Sankaran, Singh, N.P., Chander Datt, Santhosh, B., Nedunchezhiyan, M., Laskar, S.K. and Ngachan, S.V. 2008 Evaluation of high yielding cassava varieties under upland conditions of Tripura J. Root Crops 34: 73-76.

     

    3.86
    17 M. Sankaran, Singh, N.P., Chander Datt, Santhosh, B., Nedunchezhiyan, M., Laskar, S.K. and Ngachan, S.V. 2008 Morphological and proximate composition of Amorphophallus muelleri Blume J. Root Crops 34: 79-81.

     

    3.86
    18 Chander Datt, Singh, N.P., Aruna Chhabra, Bujarbaruah, K.M., and Dhiman, K.R. 2009 Nutritional evaluation of cultivated fodders grown under agro-climate of Tripura Indian J. Anim. Sci. 79: 1143-1148.

     

    6.28
    19 Kamble, A.B., Kundu, S. S., Shelke, S.K., Chander Datt and Nisha Jha. 2009 Evaluation of concentrate feedstuffs for carbohydrate and protein fractions and in vitro methane production Indian J. Anim. Nutr. 62: 116-121.

     

    5.02
    20 Chander Datt, G.V.N. Reddy and S.S. Kundu 2011 Effect of feeding differently processed paddy straw based complete diets on feed intake and nutrient utilization in Nellore lambs Indian J. Anim.Nutr. 28: 160-165.

     

    5.02
    21 Vinod Kumar, Monica Puniya and Chander Datt. 2011 Effect of heavy metals on in vitro rumen fermentation and use of lignosulfonate as an ameliorant. Indian Vet. J. 88: 77-79.

     

    22 Shete, S.M., Rijusmita Sarma, Chander Datt and S.K. Tomar. 2012 Effect of syringe type on rumen fermentation parameters while using in vitro gas production technique Indian Vet. J. 89:32-33.

     

    23 Chander Datt, S. Malik, M. Datta, N.P. Singh and S.S., Kundu

     

    2011 Effect of probiotics supplementation on feed consumption, nutrient digestibility and growth performance in crossbred pigs under Tripura climate Indian J. Anim. Nutr. 28: 331-335.

     

    5.02
    24 Avijit Haldar, Soumen Pal, Rajesh Pal, S. Pan, C. Biswas, D. Majumdar, Chander Datt, K.M. Bujarbaruah, M. Datta and B.S. Prakash 2012 Immunological physiological validation of an enzyme linked immunosorbent assay (ELISA) for the measurement of growth hormone in goat (Capra hircus) plasma Small Rumin. Res. 104: 163-168.

     

    6.97
    25 Jha, Nisha, Chander Datt  and S.S. Kundu 2011 Effect of protein sources and sodium sulphate supplementation on methane emission from berseem based rations Indian J. Anim. Nutr. 29: 355-362.

     

    5.02
    26 Jha, Nisha, Sachinandan De, S.S. Kundu and Chander Datt 2013 Identification of methanotrophs in the buffalo rumen by 16S rRNA sequence analysis Indian J. Anim. Sci.

     

    83: 1323-1327 6.28
    27 Sonali Prusty, Nisha Jha, S.S. Kundu, Ajay Kumar, P. Bala and Chander Datt 2012 Comparative nutritional evaluation of chicory, berseem and oats fodders in growing buffalo calves Indian J. Anim. Nutr. 29: 157-160.

     

    5.02
    28 Chander Datt, S.Malik, Avijit Haldar, N.P. Singh, M. Datta and S.S. Kundu 2013 Effect of probiotics supplementation on egg quality and production performance in Gramapriya birds under warm and humid climate  Indian J. Anim. Nutr. 30: 320-324.

     

    5.02
    29 Malik, S., S. Kumar, Chander Datt and N.P. Singh

     

    2011 Evaluation of D08 chicken variety in field and farm conditions of Tripura Indian J. Poultry Sci. 46: 35-39.

     

    4.35
    30 Chander Datt, Ajay Kumar and S.S. Kundu 2013 Effect of different levels of added selenium without or with arsenic on rumen fermentation parameters in buffaloes under in vitro conditions. Indian J. Anim. Sci. 83:1203-1206.

     

    6.28
    31 Chander Datt, S. Malik and S.S. Kundu 2013 Effect of substitution of maize with wild sotti (Curcuma zeodaria) rhizomes in the diets of crossbred pigs on feed intake, growth and feed conversion ratio Indian  Vet. J. 90: 37-38
    32 Sonali Prusty, S.S Kundu, Ajay Kumar, A P Ruhil and Chander Datt 2013 Quantitative prediction of indigestible cell wall fraction of tropical forages used in livestock feeding Indian J. Anim. Sci. 83:1347-1350

     

    6.28
    33 Sonali Prusty, Madhu Mohini, S.S. Kundu, Ajay Kumar and Chander Datt. 2013 Methane emission from Indian buffaloes fed on green fodders in relation to the nutrient intake and digestibility Trop. Anim. Health Prod.

     

    DOI 10.1007/s11250-013-0447-7. 6.98
    34 Dinesh Kumar, Chander Datt, L. K. Das, S. S. Kundu and V. K. Sharma 2013  Effect of different dietary protein levels on feed intake and blood parameters profile in growing Sahiwal calves Indian J. Anim. Nutr.

     

    30: 370-373 5.02
    35 Malik, S., Kumar, S., Sarkar, B., Doley, S., Chander Datt and Datta, M.

     

    2013 Evaluation of Tripura Brown, a native poultry germplasm at the organized farm and field conditions Indian Vet. J. 90: 26-29
    36 Chander Datt, A. Kumar, S. Prusty and S.S Kundu 2014 Effect of supplementation of arsenic and pentasulphate mixture in the diets of murrah buffaloes given high level of selenium on blood selenium and erythrocyte glutathione peroxidase activity J. Anim. Plant Sci. 24 (Suppl. 1):12-15 6.41
    37 Prusty S, Kundu S.S., Kumar A, Sontakke U.B., Datt C, Sharma V.K., Mondal , G. 2013 Dry matter and neutral detergent fiber degradation kinetics of roughages in relation to carbohydrate and protein fractions Indian J. Anim. Nutr.

     

    30: 374-380.

     

    5.02
    38 Vimlesh, C. Sharma, Mahesh, M.S., Madhu Mohini, Chander Datt and V.M. Namoothiri. 2014 Nutrient utilization and methane emissions in Sahiwal calves differing in residual feed intake. Archieves  Anim. Nutr. http/dx.doi.org.10.1080/1745039X.2014.951193.

     

    7.89
    39 Bala, P.A., Kundu, S.S., Chander Datt, Rani, R.R. and Sonali Prusty 2015 Comparative digestibility of forages by cattle and horses Vet. Practitioner 16: 4-6. 4.55
    40 Bala, P.A., Kundu, S.S., Chander Datt, Rani, R.R. and Sonali Prusty 2015 Nutrient evaluation of leguminous and non-leguminous fodder on CNCPS basis for horses Vet. Practitioner 16: 121-123 4.55
    41 Alka Kumari, Praveen Kumar, Ezaz Ahmad, Magan Singh, Rakesh Kumar, R.K. Yadav, Chander Datt and Anil Chinchmalapure

     

    2014 Fodder yield and quality of oats fodder (Avena sativa) as influenced by salinity of irrigation water and applied nitrogen levels Indian J. Anim. Nutr. 31: 266-271 5.02
    42 Das, L.K, Kundu S.S., Kumar D and Chander Datt 2015 Fractionation of carbohydrate   and protein content of some forage feeds for ruminants for nutritive evaluation Vet. World 8 (2): 197-202

     

    5.71
    43 Lalatendu Keshary Das, S.S. Kundu, Dinesh Kumar and Chander Datt 2014 The evaluation of metabolizable protein content of some indigenous feedstuffs used in ruminant nutrition Vet. World 7: 257-261 5.71
    44 Govind Kumar, Magan Singh, Rakesh Kumar, R.K. Yadav, Chander Datt, K. Paul,, P.G. Soni and A. Chauhan

     

    2015 Yield and quality of fodder turnip as affected by nitrogen application and weed management during lean period Indian J. Anim. Nutr. 32: 57-62 5.02
    45 Lalatendu Keshary Das, S.S. Kundu, Chander Datt and Dinesh Kumar 2014 Nutritional evaluation of some forage feeds of ruminants using TDN based model of National Research Council- 2001 The Ecoscan 8: 367-373

     

    4.65
    46 Dinesh Kumar, Chander Datt, L.K. Das and S.S. Kundu 2015 Evaluation of various feedstuffs of ruminants in terms of chemical compositioin and metabolisable energy

     

    Vet. World 8: 605-609. 5.71
    47 Tegene Negesse, Chander Datt and S.S. Kundu 2016 Variability in residual feed intake and nutrient utilization in Murrah buffalo heifers Trop. Anim. Health Prod. DOI:10.1007/S11250-016-1130-6. Published online on Sept. 12, 2016

     

    6.98
    48 Kuldeep Dudi and Chander Datt

     

    2015 Relationship of residual feed intake with blood metabolites and hormones in Sahiwal female calves. Forage Res. 41: 40-45  4.48
    49 Kuldeep Dudi, Chander Datt, Madhu Mohini, Indu Devi, V.K. Sharma and S.S. Kundu 2016 Nutrient utilization and enteric methane emissios in female Sahiwal calves of varying residual feed

     

    Indian J. Dairy Sci. 69: 555-559 5.26
    50 Ph. Suraj Sharma, Chander Datt, Bhong Nana Baban, S.S. Kundu, Nitin Tyagi and Vijay Kumar Sharma 2016 Effect of inclusion of different levels of culled potatoes in replacement of maize in the concentrate mixture on feed intake, nutrient utilization and growth in Sahiwal calves Indian J. Anim. Nutr.

     

    33: 17-21. 5.02
    51 Lalit Budhani, Madhu Mohini, Chander Datt and S.S. Thakur 2016 Effect of particle size of paddy straw on nutrient utilization and enteric methane emissions from Murrah buffalo calves Indian J. Anim. Nutr. 33:11-16.

     

    5.02
    52 Taramani Yadav, Nisha, K. Chopra, N.K. Chopra, Rakesh Kumar, Magan Singh, Chander Datt, P.G. Soni, Deepak Kumar Rathore and Sourabh Kumar 2016 Influence of weed control methods on yield and quality of cowpea fodder Indian J. Anim. Nutr.

     

    33: 70-74. 5.02
    53 Dinesh Kumar, Chander Datt, L.K. Das, P.K. Yadav and S.S. Kundu 2016 Effect of   dietary protein levels on performance, nutrient balance, plasma enzymes and   hormones profile of   growing Sahiwal calves

     

    Indian J. Anim. Res. 6: 33-39. 6.20
    54 Rakesh Kumar, Sourabh Kumar, Ashutosh, Magan Singh, B.S. Meena, S.K. Tomar,   Chander Datt, Hardev Ram and D.K. Rathore 2016 Performance of quality protein   maize under different planting windows Indian J. Anim. Nutr. 33: 154-159 5.02
    55 Vijay K. Sharma, S.S. Kundu, Sonaly Prusty, Chander Datt and Muneendra   Kumar 2016 Nutrient utilisation, growth performance and blood metabolites in Murrah   buffalo calves   (Bubalus bubalis) divergently selected for residual feed intake Arch.     Anim. Nutr. 70: 455-469 7.89
    56 M.L Kamboj, R.A. Mengistu, S. Bindal and Chander Datt 2016 Challenge feeding and   milk production performance in crossbred cows

     

    Indian J. Anim. Nutr. 33: 285-289 5.02
    57 Pooja Gupta Soni, R.K. Yadav, Ashwani Kumar, Rakesh Kumar, Chander Datt,   Koushik Paul and Govind Kumar 2016 Sorghum fodder production and its nutrient   composition under different residual sodium carbonate levels in irrigation water Indian  J. Anim. Nutr.  33: 345-349 5.02
    58 B. Patel, N. Kumar, V. Jain, H.M. Ajithakumar, S. Kumar, N. Raheja, S.S. Lathwal, C.   Datt and S.V. Singh 2017 Zinc supplementation improves reproductive performance    of  Karan-Fries cattle Indian J. Anim. Reprod. 38: 20-22 3.43
    59 M. Sujata Hegde, Jaisunder, A. Kumaresan, Chander Datt, Pawan Singh and Avtar     Singh 2016 Micro   mineral concentration in soil, plant and dairy cattle in Andaman   Group of  Islands Indian J. Anim. Nutr. 33: 394-398.

     

    5.02
    60 Negesse T, Chander Datt and Kundu, S.S. 2017 Residual feed intake, digestibility of   nutrients and efficiency of water utilizations in Murrah buffalo heifers J. Dairy Vet.   Anim. Res. 5 (2): doi: 00138. DOI: 10.15406/jdvar.2017.05.00138
    61 Sakshi Sindwani, Bhawana Chanana, Simmi Bhagat and Chander Datt 2017   Chemical   composition of bhimal fibres Int. J. Curr. Adv.  Res. 6: 5343-5347 Impact factor: SJF-5.99
    62 Jawid Sediqi, Alkesh Chaudhari, Nitin Tyagi, Sachin Kumar, Ravinder Malhotra,   Chander Datt and A.K. Tyagi 2018 Influence of levels of metabolisable protein in   the ration on nutrient utilization and lactation performance in Murrah buffaloes Indian    J. Anim. Nutr. 35: 31-36 5.02
    63 Sharma, VK, Kundu, S.S, Chander Datt, Prusty, S, Kumar, M. and Sontakke, U.B. 2018   Buffalo heifers selected for lower residual feed intake have lower feed intake, better   dietary nitrogen utilisation and reduced enteric methane production J. Anim. Physiol   Anim.   Nutr. 102: 607-614 7.61
    64 Ravi Prakash Pal, Veena Mani, Deepika Tripathi and Chander Datt

     

     

    2018. Inorganic   vanadium supplementation in crossbred calves: Effects on antioxidant status, immune   response and haemato-biochemical attributes Biol. Trace Element   Res.

     

    https://doi.org/10.1007/s12011-018-1295-0 8.36
    65 Gaurav Thorat, Anjali Khare, Lathwal, S.S. Mamta Joyswal, A. Aziz and Chander Datt 2018 Effect of dietary micronutrients on physilogical responses and biochemical   profile in growing cross bred calves Int. J. Current   Microbiol. Appl. Sci.  7 (07): 773-782
    66 D.S. Narvariya, Ramesh Chandra, Nishant Kumar, A.K. Roy,  Chander Datt and R.K.       Mehla 2018 Supplementary feeding:  Effect on growth and blood metabolites in           Sahiwal heifers Indian J. Anim. Nutr. 35: 161-165 5.02
    67 Rajesh Kumar, Ravi Prakash Pal, Veena Mani, Neelam Kewalramani, Chander Datt and Digvijay Singh 2018 Effect of inorganic vanadium supplementation on mineral balances and their circulatory levels in Karan Fries calves. Indian J. Anim. Nutr. 35: 260-265.

     

    5.02
    68 M. Sujata Hegde, Jaisunder, Pawan Singh and Chander Datt 2018 Macro mineral status in soil,   plant and dairy cattle in Andaman group of islands Indian J. Anim. Nutr. 35: 415-420

     

    5.02
    69 M. Vinceti, R. Chawla  T. Filippini, C. Dutt, S. Cilloni, R. Loomba, A. Bargellini, N.   Orsini, K.S. Dhillon  and  P. Whelton 2019 Blood pressure levels and hypertension prevalence in a high selenium environment: results from a cross-sectional study Nutrition, Metabolism & Cardiovascular   Diseases 29: 398-408 NA
    70 Digvijay Singh, Chander Datt, Akash Mishra, Swati Shivani, Ritika Gupta and Veena    Mani 2019 Effect of dietary vanadium supplementation on growth performance,             mineral balance and antioxidant activity in male Sahiwal calves Indian J.  Anim. Sci. 89: 543-548 6.28
    71 Avinesh Sharma, Chander Datt,             Ritika             Gupta, Jitendra Kumar, Shambhvi, A.K.             Tyagi and V. Mani 2019 Effect of supplementation of Kappaphycus alvarezii based             seaweed product on rumen fermentation parameters under in vitro conditions Indian J. Dairy Sci. 72:514-517 5.26
    72 Rinchu Loomba, T. Filippini, R.r Chawla, Rohit Chaudhary, S.Cilloni, Chander Datt, Shavinder Singh, K.S. Dhillon and Marco Vinceti. 2019 Exposure to a             high selenium environment in Punjab, India: effects on blood chemistry Sci.

    Total Env.(Accepted)

     

    10.61

    Dr. Ajay Kumar Dang

    Google Scholar Link: ORCID 0000-0002-7306-5302

    https://scholar.google.com/citations?user=XYNwpKoAAAAJ&hl=hi

    Training undergone:

     

    S.

    No.

    Name of course Organization Duration Nature of course
    1.       Refresher course in recent advances in Agricultural research project management NAARM, Hyderabad (21 days)

    18th Sept, 2002 to 08 Oct., 2002

    Research project management cycle, Monitoring and Evaluations of research projects, Impact assessment of agricultural research
    2.       Recent Advances in Expression Genomic Techniques. NDRI, Karnal (21 days) Feb. 20th to March 12th 2007. Extraction of RNA and gene expression studies
    3.       Advance training under DST-DAAD project Technical University Munich, Germany (30 days) 14th Oct. 2009 – 12th Nov.  2009 Mechanisms of cholesterol transfer into milk-a comparative gene expression and metabolism study in buffaloes and cattle.
    4.       Advance training under DST-DAAD project Technical University Munich, Germany (30 days) 5th Oct. 2010 – 3rd  Nov. 2010 Isolation and culture of mammary epithelial cells

    Externally funded projects:

    S. No Title of Project Funding

    Agency

    Role
    1. Modulating the immune-cellular components and their signalling molecules in bovine colostrum and milk after micro-nutrient interventions and their functional validation under ex-vivo, in vivo animal models DBT P.I.
    2. Neutrophils as potential biomarkers/ predictors  of  early  pregnancy in cows DBT P.I.
    3. Assessment of early pregnancy by analysis of neutrophil  dynamics and gene expression in dairy cattle DBT P.I.
    4. Molecular basis for modulation of immunocompetence of neutrophilic function in vivo and in vitro of high producing cows and buffaloes peripartum DBT P.I.
    5. Whole genome based SNP mining and development of breed signatures for dairy and dual-purpose indigenous cattle DBT CCPI
    6. Development of Phytopharmaceutical product for Bovine Mastitis CSIR-DBT CCPI
    7. Applications of probiotics in alleviating allergic responses and  immunosenescence DBT Co-PI
    8. Cloning, expression and functional characterization of a mammary gland protein MGP-40 expressed during involution and exploring its potential as a prognostic mammary biomarker in farm animals DBT Co-PI
    9. Analysis of mammary gland transcriptome and proteome during lactation and involution in indigenous cattle and buffalo for identification of probable mammary biomarkers NAIP

     

    Co-PI
    10. Mechanisms of cholesterol transfer into milk-a comparative gene expression and metabolism study in buffalo and cattle DST-DAAD Co-PI
    11. Role of probiotic lactobacilli in modulation of intestinal epithelium mediated barrier functions and immune signals DBT Co-PI
    12. Confirmation of lactation performance and safety of dairy animals of Bos species treated with recombinant bovine somatotropin (rBST) Consultancy

    (ELANCO)

    Co-PI
    13. Testing Jeevan  Pashudhan (Penergetic  T), Jeevan Pashudhan Ultra (Penergetic M) for improved immunity, prevention of mastitis in dairy animals Consultancy

    (PANERGETIC)

    Co-PI

     Awards:

    Sr. No. Name of Award Year Awarding Body
    1.       Young Scientist Award

    (Ph.D. student)

    2021 SAPICON 2021 (25-26 February, 2021) Bihar Veterinary College, Patna
    2.       2nd Best Oral Paper Presentation Award 2020 National Conference held at Post Graduate Institute of Veterinary Education and Research, Jaipur during February 4-6, 2020.
    3.       Dr. (Mrs.) Mridula Kamboj Young Scientist  Best Doctoral Thesis Award to Ph.D. Student 2019 9th Annual Meeting of the ISSRF held at Jawaharlal Nehru University, New Mehrauli Road, New Delhi-110067
    4.       J.N.Pandey Memorial Best poster award 2018 Society of Animal Physiologists of India (SAPI) Conference, 2018
    5.       Best research paper award 2018 SAPI Conference, 2018
    6.       SC Sood Memorial Best Doctoral Thesis Award to Ph.D. Student 2018 SAPI Conference, 2018
    7.       Second  prize for best paper and

    Rs. 3000/-

    2017 45th Dairy Industry Conference held at Mumbai by Indian Dairy Association (IDA)
    8.       J.N.Pandey Memorial Best poster award for best Poster SAPI- (Mhow) 2016 SAPI Conference, 2016
    9.       Best Master’s thesis award in the Production group to Master’s student 2015 ICAR-NDRI, Karnal
    10.   Best Research Paper Award 2014 2nd Annual Meeting of Society of Veterinary Science and Biotechnology
    11.   Best  Poster  Paper  Award 2014 9th  National Convention of Indian Dairy  Engineers Association  (IDEA)
    12.   Kamala Madan Young Scientist Best Doctoral Thesis Award to Ph.D. Student 2014 SAPI Conference, 2014
    13.   Best  Poster  Paper  Award 2013 2nd National  IAVNAW Conference, Jammu
    14.   Best poster paper 2007 International Conference on Dairy Foods, NDRI, Karnal
    15.   First prize for Technical Bulletin in Hindi 2005 ICAR-NDRI, Karnal
    16.   Second Prize for Best Paper 2005 Indian Dairy Assoc.,New Delhi
    17.   Dr. D.N. Mullick Memorial Award 2002 Alumni Association, NDRI, Karnal
    18.   Dr. P.K. Dwarkanath Young Scientists Memorial Award 2002 Alumni Association, NDRI, Karnal
    19.   Dr. D. Sunderasen Memorial Award 2002 Alumni Association, NDRI, Karnal

     Consultancy provided:

    Estimation of Total immunoglobulins, IgG, IgA and IgM in dry milk colostrums powders to Supreme Dairy Farm, Mumbai and Merck, Ltd., Worli, Mumbai. Training on estimation of milk SCC provided to Gopal Jee Dairy Foods, VRS Foods Limited, PARAS Dairies.

    Research papers published (Ten best publications of last 5 years):

    • Alhussien, M.N., Tiwari, S., Panda, B.K., Pandey, Y., Lathwal, S.S. and Dang, A.K. (2021) Supplementation of antioxidant micronutrients reduces stress and improves immune function/response in periparturient dairy cows and their calves.Journal of Trace Elements in Medicine and Biology, p.126718. (NAAS rating 2021: 9.25)
    • Alhussien, M.N. and Dang, A.K. (2020)Sensitive and rapid lateral-flow assay for early detection of subclinical mammary infection in dairy cows. Scientific Reports,10(1), p.11161. (NAAS rating 2021: 10.00)
    • Panda, B.S., Mohapatra, S.K., Chaudhary, D., Alhussien, M.N., Kapila, R. and Dang, A.K. (2020) Proteomics and transcriptomics study reveals the utility of ISGs as novel molecules for early pregnancy diagnosis in dairy cows. Journal of Reproductive Immunology, 140, p.103148. (NAAS rating 2021: 10.02)
    • Mohapatra, S.K., Panda, B.S., Verma, A.K., Kapila, R. and Dang, A.K. (2020) Implantation associated changes in expression profile of indoleamine-2, 3-dioxygenase 1, Th1-Th2 cytokines and interferon-stimulated genes on neutrophils and peripheral blood mononuclear cells of crossbred cows. Journal of Reproductive Immunology, 142, p.103188. (NAAS rating 2021: 10.02)
    • Panda, B.S., Mohapatra, S.K., Verma, A.K., Kamboj, A., Alhussien, M.N. and Dang, A.K. (2020). A comparative study on various immunological parameters influencing embryo survivability in crossbred dairy cows. Theriogenology, 157, pp.140-148. (NAAS rating 2021: 8.09)
    • Manjari, P., Hyder, I., Kapoor, S., Senthilnathan, M. and Dang, A.K. (2018) Exploring the concentration‐dependent actions of interferon‐τ on bovine neutrophils to understand the process of implantation. Journal of Cellular Biochemistry119(12), pp.10087-10094 (NAAS rating 2021: 10.24)
    • Alhussien, M.N. and Dang, A.K. (2018) Impact of different seasons on the milk somatic and differential cell counts, milk cortisol and neutrophils functionality of three Indian native breeds of cattle. Journal of Thermal Biology, 78. 10.1016/j.jtherbio.2018.08.020.(NAAS rating 2021: 8.36)
    • Alhussien, M.N. and Dang, A.K. (2018) Pathogen-dependent modulation of milk neutrophils competence, plasma inflammatory cytokines and milk quality during intramammary infection of Sahiwal (Bos indicus) cows. Microbial Pathogenesis, 121:131–138.(NAAS rating 2021: 8.91)
    • Sheikh, A.A., Hooda, O.K. and Dang, A.K. (2017) JAK3 and PI3K mediates bovine Interferon-tau stimulated gene expression in the blood neutrophils. Journal of Cellular Physiology (DOI: 10.1002/jcp.26296).(NAAS rating 2021: 11.55)
    • Panchal, I., Sawhney, I.K., Sharma, A.K. and Dang, A.K. (2016) Classification of healthy and mastitis Murrah buffaloes by application of neural network models using yield and milk quality parameters. Computers and Electronics in Agriculture, 06/2016; 127:242–248.(NAAS rating 2021: 9.86)

    Research Publications: 60

    Laboratory Manuals: 3

    Technical Books: 5

    Popular articles: 20

    Teaching as Course Incharge:

    Sr. No. Course No. Theory /Practical
    1.    AP-611 (Hematology, Cardiovascular and Respiratory Physiology) Theory and Practical
    2.    AP-622  (Physiology  of  Lactation) Theory and Practical
    3.    AP-714 (Advances  In  Biosynthesis and Secretion of Milk) Theory
    4.    AP-724 (Advances in Immunophysiology) Theory

    Dr. Sohan Vir Singh

    Qualifications:

    • M.Sc.(Animal Physiology)
    • Ph. D.( Animal Physiology)

    Major Research Accomplishments

    • Work performance of lactating non pregnant, dry non pregnant cows and buffaloes, indigenous and crossbred male bullocks were carried out during ploughing and carting. Animals were monitored for different hormones during work and rest.
    • Cutaneous skin blood flow in cattle and buffaloes were monitored during treadmill exercise during summer and winter season. Blood flow to periphery increased significantly due to treadmill exercise compared to resting conditions.
    • On the basis of gradient of change in physiological responses, skin temperature and milk productivity, it was observed that the minimum variations took place between 15-25°C in KF and Murrah buffaloes and 15-28°C in Sahiwal cattle.
    • The total immunoglobulin (Ig) levels were found to be higher during winter than summer season in Karan Fries cows. The level of total Ig was significantly lower (P<0.01) in mastitic and wounded animals on the day of detection compared to after treatment during both the seasons.
    • The productivity of animals was related to climate change in terms of ambient dry bulb, maximum temperature and Temperature Humidity Index. The milk production decline in Zebu and crossbred cows and buffaloes with increase in temperature humidity index.
    • Expression profile of major heat shock protein genes during different seasons in cattle and Murrah buffaloes under tropical climatic conditions     were carried out.
    • Adaptative genetic and physiological changes in integument, fibroblast cells and melanocytes of cattle during different seasons were carried out.
    • Seasonal variation in metabolic activity and related gene were studied in Tharparkar and Karan Fries heifers
    • Poor quality of semen and their amelioration by supplementing astaxanthin (potent herbal antioxidant) to Karan Fries breeding bulls during summer stress.
    • Insight into thermal adaptations of Tharparkar and Karan Fries cattle through thermal imaging during summer season were studied.
    • Mitigation of heat stress using custom design shelter system and supplementation of chromium propionate to growing Karan Fries cattle.
    • Influence of astaxanthin supplementation on growth performance, immunity and antioxidant status of Karan Fries heifers during summer season.
    • Effect of melatonin implant on productive and reproductive performance of Murrah buffaloes were carried out during summer season
    • Effect of astaxanthin and prill fat supplementation on energy metabolites, stress markers and milk production in rural buffaloes during summer season.
    • Effect of betaine supplementation on growth performance, feed intake and stress biomarkers status of buffalo heifers.

    Guidance to student:

    • M.Sc/ M.V.Sc : 16
    • Ph.D. : 05

    Awards:

    Sr. No. Name of the award/ prize Subject work Awarding Agency Year of award
    1. First Best Research article award Identification  of SNPs in ATP1A1 gene and their association with heat tolerance in Sahiwal and Karan Fries (Bos Taurus x Bos indicus) cattle under tropical climatic condition” Indian Dairy Association 2020
    2. First prize in oral presentation “Physiological responses, energy metabolites and prolactin levels of buffaloes with dietary astaxanthin, prill fat and their combination during heat stress” Association of Agrometeorology 2020
    3. Best poster presentation award A potential non- invasive tool for maping of surface temperature of buffaloes during summer season” Indian Dairy Association 2020
    4. Young Scientist Award Transitional Sahiwal Adapt Better than Karan Fries (Crossbred) Cattle during summer and Winter Seasons Association of Animal Scientists 2020
    5. Fellow Fellowship of Animal Physiologists Association (APA) Animal Physiologists Association 2019
    6. Felicitation & Release of book National Use of Weather Forecast for Livestock Management in Agrometeorological Services IMD, New Delhi 2018
    7. Best poster presentation award to my M.V. Sc student Effect of dietary supplementation of astaxanthin and prill fat to lactating buffaloes for amelioration and sustained productivity during heat stress Animal Physiologists Association 2019
    8. Best M.V. Sc thesis award to my M.V. Sc student Effect of astaxanthin and prill fat supplementation on energy metabolites, stress markers and milk production in rural buffaloes during summer season Animal Physiologists Association 2019
    9. Best Ph.D. Thesis Award to my Ph.D. student Influence of astaxanthin supplementation on growth performance, immunity and antioxidant status in heifers during summer season Animal Physiologists Association 2018
    10. Best Poster Presentation Award Effect of seasons on energy metabolites in transition Sahiwal and Karan Fries cows Animal Physiologists Association 2018
    11.

     

    Second Best Oral Presentation Award Scrotal surface temperature and its relationship with body weight, body condition score and scrotal circumference in Murrah buffalo breeding bulls Indian Society for Animal Production & Management 2016
    12. Second Best poster presentation award “ftad vuqiwj.k dk dkj.k Qzht xk;ksa ds iztuu izn”kZu” ICAR-NDRI, Karnal 2016
    13. Second Best poster presentation award Effect of Astaxanthin on semen quality of Karan Fries bulls during hot dry and hot humid seasons Animal Nutrition Society of India 2016
    14. Best oral presentation award Seasonal variation of seminal antioxidant enzymes concentration in crossbred (Tharparkar X Holstein Fries) Bulls under tropical climatic conditions Society for Animal Physiologists of India 2016

    Patents, Technology, Methodology, Genetic Stock, Variety, etc.:

    • Demarcation of the ranges of ambient temperature (thermoneutral zones) for Indian cattle, Zebu crosses (Karan Fries and Karan Swiss) and buffaloes.
    • Developed methodology for alleviating thermal heat using fan cum mist system. Angular high velocity blasting fan were used for effective heat dissipation and sustaining milk production and improving feed intake in crossbred cows and buffaloes during hot dry and hot humid months.
    • Developed work performance indices for Haryana and crossbred bullocks during carting based on Oxygen /pulse/kg body weight.
    • Developed a map to demarcate the different zones of India for stress and comfort based on temperature humidity index (THI).
    • Developed a methodology to monitor methane emission in ruminants.

    Research publications having NAAS rating equal to or more than 6 only

    1.  Kumar, S. and Singh, S.V. (2020) Influence of astaxanthin supplementation on attainment of puberty and lipid peroxidation in Sahiwal and Karan Fries (Holstein × Tharparkar) heifers during summer season. Biological Rhythm Research 51(1): 15-28 . (NAAS Rating: 6.77)
    2. Kumar, S., Singh, S.V. and Bhan, S.C. (2019) Effect of dietary supplementation of astaxanthin (potent antioxidant) on growth rate, DMI, FCR and metabolic changes in Karan Fries heifers during heat stress. Journal of Agrometeorology 21 (1): 80-88. (NAAS Rating: 6.64)
    3. Kumar, S. and Singh, S. V. (2019) Inhibition of NF-κB signaling pathway by astaxanthin supplementation for prevention of heat stress-induced inflammatory changes and apoptosis in Karan Fries heifers. Tropical Animal Health and Production51(5):1125-1134.(NAAS Rating: 7.09)
    4. Somagond, Y.M., Singh, S.V. and Deshpande, A. (2019): Effect of dietary supplementation of astaxanthin, prill fat and combination on stress indicators, milk yield and composition during heat stress in buffaloes. Biological Rhythm Research DOI:1080/09291016.2019.1658426. (NAAS Rating: 6.77)
    5. P., Singh, A., Singh, P., Singh, S.V., Yadav, S and Kumar, S. (2019) Scrotal infrared thermography and testicular biometry: Indicator of semen quality in Murrah breeding bulls. Animal Reproduction Science https//doi.org 10.1016/J.anireprosci.2019.106145. (NAAS Rating: 7.82)
    6. Saikia, , Verma, A., Gupta, I. D., Singh, S. V. and Hazarika, D. (2019) Identification of genetic variants in HSF1 gene and their association with heat tolerance in Murrah buffaloes. Indian Journal of Animal Sciences 89(10): 1099–1103. (NAAS Rating: 6.23)
    7. Kumar, S. and Singh, V. (2019) Heat stress amelioration by astaxanthin supplementation and Nrf2 transcribed down   regulation   of HSP70 and MnSOD   in Karan Fries  (Holstein   Friesian×Tharparkar) heifers Indian Journal of Animal Sciences 89(11): 1211–1216. (NAAS Rating: 6.23)
    8. Singh, S.V., Kumar, Y. and Kumar, (2019) Impact of temperature humidity index (THI) on physiological responses and milk yield of Tharparkar and Karan Fries cows exposed to controlled environmen. Journal of Agrometeorology 21(4): 405-410. (NAAS Rating: 6.64)
    9. Choudhary, R., Sharma, A., Kumar, S., Upadhyay, R. C., Singh, S. V., and Mohanty, A. (2019). Role of alpha-melanocyte stimulating hormone (α-MSH) in modulating the molecular mechanism adopted by melanocytes of Bos indicus under UVR stress. Molecular and Cellular Biochemistry, 465 : 141-153. (NAAS Rating: 8.88)
    10. Singh, S. V. and Naskar, S. (2019) Adaptation and mitigation strategies for sustained livestock production under changing climatic scenario. Journal of Agrometeorology 21(Special Issue-1): 15-28. (NAAS Rating: 6.64)
    11. Vaidya, M.M., Singh, S.V. and Singh, A.K. (2019) Effect of thermal stress on HSP 72 and leptin mRNA expressions and milk yield of periparturient dairy cows. Journal of Agrometeorology 21(Special Issue-1): 15-28. (NAAS Rating: 6.64)
    12. Yallappa M. Somagond, V. Singh and Aditya Deshpande (2020) Physiological responses, energy metabolites and prola levels of buffaloes supplemented with dietary astaxanthin, prill fat and their combination during heat stress. Indian Journal of Animal Sciences 90(1): 55–60. (NAAS Rating: 6.23)
    13. Aditya Deshpande, V. Singh, Yallappa M Somagond, Parvender Sheoran, Saurav Naskar And V. P. Chahal (2020) Physio-biochemical responses and growth performance of buffalo heifers to betaine supplementation during hot humid season under field conditions. Indian Journal of Animal Sciences 90 (3): 416–423. (NAAS Rating: 6.23)
    14. K. Singh,R. C. Upadhyay, Gulab Chandra, Sudarshan Kumar, D. Malakar, S. V. Singh and M. K. Singh (2020).  Genome­wide expression analysis of the heat stress response in dermal fibroblasts of Tharparkar (zebu) and Karan-Fries (zebu × taurine) cattle. Cell Stress and Chaperones 25(2): 327-344. (NAAS rating: 8.90)
    15. V. Singh And Chandra Bhan (2020) Physiological, hematological and biochemical profile of growing Murrah buffaloes exposed to heat stress in control climatic chamber. Indian Journal of Animal Sciences 90 (6): 928–933. (NAAS Rating: 6.23)
    16. Kotresh Prasad Chikkagoudara, Pawan Singh, Deepandita Barman, Carolina Potshangbam, Ninad Bhatt, Sohan Vir Singh, Surender Singh Lathwal (2020) – Eye temperature, an indicator for stress levels in young buffalo bulls – A case study of micro-environment modification. Journal of Agrometeorology 22 (3): 266-273. (NAAS Rating: 6.64)

    (ii) List of popular articles published:

    1. Sonali Prusty, Simson Soren and Sohan Vir Singh (2016) Area Specific Mineral Mixture in Farm Animal Nutrition. Indian Dairyman 68: 88-91.
    2. Sohan Vir Singh (2016) Housing and Shelter Management for Alleviating Climate Related Stress in Dairy Animal. Indian Dairyman 68: 78-81.
    3. G. Shashank, S.V. Singh, S. Grewal, and S. Soren (2017) Climate change: an entrenched rogue covering livestock sector. Indian Dairyman 69:84-88.
    4. Simson Soren, Sohan Vir Singh, Shashank, C.G. and Bharath Kumar, B.S. (2017) Heat stress in crossbred cattle bulls and ameliorative approaches under tropical climatic conditions. Indian Dairyman 69 (8): 92-96.
    5. Sohan Vir Singh and Arun Kumar Mishra (2017) Climate Smart Livestock Production Systems for enhancing economy and green environment. Indian Dairyman 69 (11): 96-100.
    6. Sunil Kumar, V. Singh, Priyanka Pandey and Suchit Kumar (2017) Endocrine status of heat stressed cows related to reproduction. Livestock Line 11:22-23.
    7. Kumar and S.V. Singh (2018) Assessment of heat stress in dairy animals under field and farm conditions. Indian Dairyman 70(8): 88-91.
    8. G. Shashank, T. Kaur, B.S. Kumar and S. V. Singh (2018) Impact of climate change on behavioral flexibility of animals. Indian Dairyman 70(10): 76-79.
    9. Sohan Vir Singh and S.C. Bhan (2019) Role of meteorological forecast in improving livestock practices and welfare. Indian Dairyman 71(6): 88-95.
    10. Sohan Vir Singh (2019) Ik”kq/ku mRiknu ij tyok;q dk izHkko ,oa cpkkoA nqX/k lfjrk 3 (4): 24-27.
    11. Sohan Vir Singhand S.C. Bhan (2019) Ik”kq/ku mRiknu ds fy, ekSle dk iwokZuqekuAnqX/k lfjrk ¼flarcj&vDVwcj½: 23-27.
    12. Sohan Vir Singh (2019) vkftfodk vkSj [kkn~; lqj{kk esa mi;ksxh HkSal ikyuA [ksrh (vDVwcj): 35-38.
    13. Mangesh M.Vaidya, Sandeep A.Dhenge, Vivek Khandait, S.M.Durge and S.V. Singh (2019) Shelter Management for Sustained Productivity in Farm Animals. Lab to Land 2 (3): 4-9.
    14. Yallappa M. Somagond, Sohan Vir Singh, Pooja Devi, V. R. Upadhyay, and Jayashree Gogoi (2020) Impact of Heat Stress on Semen Quality of Bovines. Dairy Planner 17 (2): 16-17.VVV
    15. V. R. Upadhyay, Sohan Vir Singh, Nishtha Kushwah, Yallappa M. Somagond, Gaurav Kumar and Nikita Bhalakiya (2020) Adaptability characteristics of cattle against heat stress in tropics. Indian Dairyman 72 (11): 78-81.