Significant achievements:

1. Produced World’s First buffalo Cloned calf.
2. Produced buffalo cloned calf “Garima-II” from embryonic stem cells.
3. Produced India’s first OPU-IVF Sahiwal calf.
4. Produced OPU-IVF yak in a collaborative project with NRC-Yak, Dirang (Arunanchal Pradesh)
5. Produced reproducible cloned buffalo “Garima-II”, delivered Mahima and Karishma.
6. Developed Hand guided cloning technology in goat and buffalo and in buffalo produced many superior genetically cloned buffalo calves (Garima, Shresth, Swarn, Purnima, Lalima, Rajat, Swarupa).
7. Produced cloned calf “Deepasha”from endangered wild buffalo of Chhattisgarh.
8. Produced 5 Embryonic Stem Cell lines in buffalo. These are the only lines available in the world, so far.
9. Produced GFP expressed cloned transgenic Goat and Buffalo embryos.

Technologies developed:

1. In vitro embryo production (IVF) Technology in goat, Buffalo & yak, implemented in all over the country.
2. Developed new protocol of Semen cryopreservation using Egg Yok -Tris diluents in Jamunapari Goat.
3. Developed technology for Transgenic embryo production in Goat an Buffalo using hand guided cloning.
4. Developed hand guided Cloning Technology in Goat and Buffalo.
5. Developed Embryonic Stem Cell Culture Technology in Goat and Buffalo.
6. Ovum –Pick up Technology in Cattle.
7. Developed Protocol for Freezing of Goat Semen & Artificial Insemination and commercialized.

                               These technologies brought the ‘Name & Fame’ to ICAR & India.

Research & Development -Projects handled: 19 (as Principal Investigator (PI): 12, Co-Pi: 9)

1. Principal Investigator (PI) of Department of Biotechnologies (Govt. of India) funded projects; 10
2. National Agricultural Innovation Projects (ICAR): 2, (PI:1, Co-Pi: 1)
3. National Fund Project: 3, (PI:1, Co-Pi: 2)
4. Indo- German project granted by DST (Govt. of India & Germany)Co-Pi: 1
5. DBT (Govt. India) & other agency: 5

Multi-disciplinary and multi-Institute research/network programmes:

1. Inter Institutional Research Projects (Collaborative project with: IISC, Bangalore, AAU Guwahati, GADVASU, Ludhiana, CIRC, Meerut, ICAR, RC Barapani, ): 5
2. Inter National project collaboration (FBN Dormstoff, Germany): 1
3. Monitoring DBT project as a Task Force member of Committee: 3

Foreign Visits: Foreign deputation (USA, Germany, Canada)- 4 times as different capacities

Inter disciplinary programmes and participation in research activities

1. PI of 11 out funding research projects (DBT, DST, NAIP, National Fund, ICAR )
2. Task Force Member of 3 Research & Development Committees of Department of Biotechnology (Govt. of India), New Delhi from 2012 onwards.
3. Member of a Team Constituted by Hon’ble DG, ICAR, New Delhi for development Booklet on Agricultural Biotechnology for Hon’ble Member of Parliament.
4. Reviewer of  all the reputed journals- Theriogenology, Cellular Reprogramming, Reproduction of Domestic Animals and Indian Journal- Ind. J. Animal Sciences, Ind. J. Experimental Biology, Ind. J. of Biotechnology.

Teaching & Guidance:

1. Guided 10 PhD & 9 Master degree students in Reproductive Biotechnology area..
2. Taught 22 courses (PhD &MVSc) at at NDRI (Deemed University), Karnal and IVRI (Deemed University), Izatnagar.
3. Developed 5 New Courses for PhD & Masters’ degree.
4. Mentor in DST sponsored INSPIRE programme in Biological Sciences.
5. Evaluated 21 PhD thesis of different universities.
6. Delivered 35 lecture in INSPIRE (DST, Govt. of India) program.

Conference Organized:

1. Chairman & Convener of National Seminar on Small Ruminants, CIRG, Makhdoom 2018
2. Organizing Secretary of 12^th Agricultural Science Congress (over1800 participants)
3. Organizing Secretary of one International Conferences (ISSRF-2011) and over 900 participants have attended.
4. Organizing Secretary one National Conference Reproductive Biology, 2012 (ISSRF) (over 500 participants)
5. Organized 5 Training programmes on Reproductive technologies (21 days) for faculty/ scientists. From SAUs, Vet. Univ., CAUs, Universities.
6. Delivered Key note address (7) and lead lectures (29) and act as Chairman Scientific Session (6) in many International Conferences, National Conferences.

Training Organized/ imparted:

1. Organised 10 National Trainings for faculty/ Scientists (Course Director of 6 Training programme and Co-Course Director of 5 Training Programme)
2. Developed 5 Training manuals.
3. Course Director of 18 Training Programmes (45 days each) for Biotechnology students from different Institution/ universities and trained 514 students.
4. Over 800 framers were trained during mu Director position at CIRG, Makhdoom.

Funds Generated/ Mobilization of Fund (as Principal Investigator and Co- PI):

           Rs. 3087.76Lakh

As PI: 306.058 Lakhs fund has been generated from the 8 out funding projects granted to me as PI by Department of Biotechnology (DBT), (Govt. of India), New Delhi; Rs.755 lakh from NAIP & NFBSFARA, NASF; over 32 lakh through consultancy.

Awards, Fellowship, Honors & Recognition:

1. Fellow of National Academy of Agricultural Sciences (NAAS).
2. Fellow of National Academy of Dairy Sciences (NASI).
3. P. Bhattacharya Life Time Achievement Award by National Academy of Sciences (NAAS), 2021.
4. Rafi Ahmed Kidwai Award by ICAR, New Delhi in 2015 (Highest Award of ICAR)
5. ICAR-Team Awardfor outstanding contribution in Animal Sciences 2014.
6. VASVIK Industrial Award in Agricultural Sciences, 2015 by Vividhlaxi Audyogik Samshodhan Vikas Kendra (VASVIK) organization established in 1974 and run by the Patel Extrusion Group, with ISO 9002 certified companies.
7. DBT Biotechnology Overseas Fellowship Award, 1997 by Department of Biotechnology, Ministry of science and Technology, Govt. of India, New Delhi.
8. Rao Bahadur B. Viswanath Award 2019 by IARI presented in convocation on Feb. 8, 2019, New Delhi.
9. Labhshwetar Award by Indian Society for the Study of Reproduction and Fertility (ISSRF)(Registration no. MS Bombay 485/1988 G.B.B.S.D), 2015.
10. Certificate of Merit Award for Exemplary Research by Virginia Tech., USA in 1999.
11. Erasmus Mundus Scholarship Awarded by EM-ABG, European Union in 2009, Executive Agency, Netherlands
12. Prof. G.P. Talwar Middle Career Scientist Award by Indian Society for the Study of Reproduction and Fertility (ISSRF)Registration no. MS Bombay 485/1988 G.B.B.S.D)
13. President of Indian Society for Sheep & Goat Production & Utilization (ISSGAPU), Avikanagar (Registered Society)
14. Certificate of Recognition by Hon’ble DG, ICAR, New Delhi.
15. Appreciation Letter by Hon’ble DG, ICAR, New Delhi on production of cloned calf Garima II.
16. Appreciation Letter by Hon’ble Agriculture Minister on production of cloned calf Garima & Mahima.
17. Member of Board on Management NDRI, Karnal, 2015.
18. Member of Institute Management Committee, NRC-E, Hisar.
19. Chairman, National Technical Committee for Breed Improvement of sheep and goats constituted by DAHDF, Govt of India dated 19^th May 2017.
20. Nominee of His Excellency Governor of Punjab for selection of Faculty for PAU, Ludhiana.  (by Principal secretary to Governor, Punjab, letter No. PRB-2G-PAU-2015/2745 dated 29.5.2015), 2015-17, office letter D.O.No. 9506 dated 18.06.2015 by the Registrar, PAU, Ludhiana
21. Fellow of Society for Extension Education Registered Professional Society award) by Society for Extension Education. Agra.
22. Visiting faculty to the Research institute for the biology of farm animals (Forschungsinstitut fur die Biologie landwirtschaftlicher Nutztiere, FBN) from September 16- October 5, 2009.
23. Visiting/adjunct Faculty at Stem cell Biology Department, National Institute for Research in Reproductive Health (ICMR), Mumbai from November 26 to December 7, 2007.
24. Visiting scientist to College of Agricultural and Environmental Sciences, Animal and Dairy Science Department, University of Georgia, USA, from April 28 to May 27, 2010
25. Eight best posters/oral presentation awards in National & International Conferences.
26. Task Force Member of 4 committees in Department of Biotechnology (Govt. India), New Delhi.
27. Mentor of Biotechnology of HNB Garhwal University (Central University) Srinagar Garhwal. (UK).
28. Member of National Selection Committee for Indo-US Fellowship for Women in stem (WISTEMM) by DST (Govt. of India), New Delhi  for 3 years. This committee is to select the women for USA Fellowship program. Letter from Dr. Namita Gupta from DST, dated 23.03.2018.
29. Recognized in Limka book of  Records 2014, Ed. Vijay Ghose ((ISBN: 978-93-82867-06-7) page3 37-338.

Research Publications:

(http://www.ncbi.nlm.nih.gov/pubmed/?term=chauhan+MS+Karnal)

https://scholar.google.com/citations?user=NSkU8fUAAAAJ&hl=en

• Research Articles: 155
• Review Articles: 12
• Books: 8
• Popular articles: 34

Other Recognition:

• Recognized by DD Kisan “Kisano ke Mahanayak (episode 18)https://www.youtube.com/watch?v=u4JLO-kLw9k
• Eureka program of Rajya Sabha TVhttps://www.youtube.com/watch?v=nipKKfhlqa0

Brief Biography: Dr. Dheer Singh is a distinguished Biochemist, Fellow of National Academy of Sciences (FNASc) and Fellow of National Academy of Agricultural Sciences, India. He received M.Sc. and Ph.D degree in Biochemistry from Lucknow University & National Dairy Research Institute (NDRI) Deemed University at Karnal (Haryana) in India, respectively. During his doctoral degree, selected as Agricultural Research Scientist (ARS) and joined Animal Biochemistry Division at NDRI as scientist and subsequently promoted to Principal Scientist and currently joined as Head, Animal Biochemistry Division. Awarded BOYSCAST (Better opportunity for young scientist in chosen area of science and technology) fellowship from Department of science and Technology (DST), Ministry of Science and Technology, Govt. of India to pursue higher study abroad and worked in Department of Cell Biology and Developmental and Anatomy at School of Medicine, University of South Carolina, USA in the area of transcriptional regulation of eukaryotic genes. Visiting scholar of School of Medicine, Stanford University, USA and worked Identification of novel endocrine genes using DNA microarray, computational tools and RNAi in functional analysis of genes in granulosa cells. Life member of prominent professional National scientific societies namely, SBC, India, ISSRF, ISCB etc. and currently, I am convener, SBC (I) Karnal Chapter. During his research career of more than 25 years, he has supervised 18 Masters and 18 Ph.D students. Handled more than 25 research project funded by various funding agencies including Indo-German joint collaborative project (DST-DFG & DST-DAAD) on epigenetic regulation of genes. Published more than 90 research papers in peer reviewed International Journals, apart from delivering more than 50 invited lectures both in India and abroad. Major focus of research of his lab is 1) Identification and analysis of the transcriptional regulation of selected trait-affecting (e.g. fertility) genes. 2) Application of miRNA & RNA interference tools for livestock functional genomics, 3) Epigenetic regulation of gene expression, 4) Exosome Biology and 5) Development of nanobiosensor.

Major Professional Awards/ Recognitions /Fellowships

• Fellow of National Academy of science, India (FNASc)
• Fellow of National Academy of Agricultural Sciences, India
• Fellow, National Academy of Dairy Sciences of India (FNADS)
• N.R. Moudgal Oration award for outstanding contribution in research in Reproductive Heath by Indian Society for Study in Reproduction and fertility (ISSRF)
• Chellappa Oration award 2014 by Indian Society of Veterinary Pharmacology & Toxicology for outstanding contribution in Animal & Veterinary Sciences
• Labhsetwar Award 2013 for outstanding contribution in research in Reproductive Heath by Indian Society for Study in Reproduction and fertility (ISSRF)
• BOYSCAST fellowship award, Department of Science and Technology, Govt. Of India
• 11^th AJAS/CAPI Outstanding Research Award by Asian-Australasian Association of Animal Production Societies (AAAP)
• 9^th AJAS/CAPI Outstanding Research Award by Asian-Australasian Association of Animal Production Societies (AAAP)
• SAB Eminent Biotechnology Award by Society of Applied Biotechnology, India
• FSAB (Fellow of Society of Applied Biotechnology).

Specific areas of Research Interest and accomplishment

• Identification and analysis of the genetic and epigenetic regulation of selected fertility related genes by functional genomics: It is well known that estrogens play key roles in the reproduction. Aromatase is the key enzyme encoded by the CYP19A1 More than a decade of my research explored the molecular biology of the CYP19 gene by uncovering its gene sequence and transcriptional regulation during physiological and pathological conditions like normal physiological ovarian cycles, pregnancy and infections in bovine model.
• Elucidating the molecular and cellular mechanisms underlying the ovarian and uterine cellular dysfunction during the infertility associated with postpartum uterine infection: Uterine infection after parturition is one of the most common endemic diseases in almost all mammals leading to infertility by disrupting uterine and ovarian functions. Using metagenomics approaches, a microorganism associated with uterine diseases was identified and developed a PCR based detection method (Patent application No. 806/DEL/2016 & Published for examination). In addition, the molecular mechanism leading to the down regulation of aromatase gene by lipopolysaccharide was elucidated, and this downregulation was limited by IGF-1 via the activation of phosphatidylinositol-dependent kinase/AKT. These findings are of practical relevance to manage the compromised follicular development by nutrition to maintain the circulating IGF1 concentrations during postpartum.
• Development of nano-biosensor for ovulation prediction: An efficient method for detecting ovulation will contribute to improved fertility both in human and animals. Although, several ovulation prediction kits are available for women, such kits are limited for animals. For example, ovulation and pregnancy detection are of labor-intensive and skill based in animals. Recently, a peptide-based antibody against Luteinizing hormone has been developed (applied for patent Application 1854/DEL/2015 & published for examination) for developing an easy and affordable lateral flow strip based nano-biosensor for estrus detection at farmers’ doors for timely AI to improve conception.
• Cell based biosensor development: We developed 3-dimensional cell culture systems for granulosa cells to better perform reproductive toxicology studies. Similarly, our studies on 3D culture systems of liver cells and intestinal cells would be helpful for toxicological evaluation of food as food is one of the major environmental factors affecting the reproduction.
• Smart delivery systems using natural nanoparticle (exosome): Milk is the natural food. In addition to nutrients, it contains the natural nanoparticles called exosomes, which act as smart delivery vehicles. Hence, milk exosomes can be used to deliver the therapeutic molecules for treating infertility. Our studies characterized cow and buffalo milk exosomes and proved that curcumin encapsulated in milk exosomes is resistant to human digestive processes and bioavailable for the uptake by the granulosa cells in vitro. In addition, we have also demonstrated that non-invasive bio-fluid exosomes miRNA would be next –generation potential biomarkers of various reproductive problems/diseases as well as drugs/nutraceuticals delivery for therapeutics.

List of important papers

1. Nayan, V., Onteru, S. K., & Singh, D. (2021). Epitope-based in silico peptide design yields peptide-directed antibodies that recognize the buffalo luteinizing hormone. International Journal of Biological Macromolecules. doi:10.1016/j.ijbiomac.2021.02.083,
2. Rani, P., Yenuganti, V. R., Shandilya, S., Onteru, S. K., & Singh, D. (2017). miRNAs: The hidden bioactive component of milk. Trends in Food Science & Technology, 65, 94-102.
3. Shandilya, S., Rani, P., Onteru, S. K., & Singh, D. (2017). siRNA in milk exosomes is resistant to digestion and cross intestinal barrier in vitro. Journal of Agricultural and Food Chemistry, 65(43), 9506-9513.
4. Shri, M., Agrawal, H., Rani, P., Singh, D., & Onteru, S. K. (2017). Hanging Drop, A Best Three-Dimensional (3D) Culture Method for Primary Buffalo and Sheep Hepatocytes. Scientific Reports, 7(1), 1203.
5. Chaurasiya V, Kumari S, Onteru SK, Singh D (2020). miR-326 down-regulate CYP19A1 expression and estradiol-17b production in buffalo granulosa cells through CREB and C/EBP-β, Journal of Steroid Biochemistry and Molecular Biology, 199, 105608
6. Monga R, Ghai S,Datta TK and Singh D (2012). Involvement of transcription factor GATA-4 in regulation of CYP19 gene during folliculogenesis and luteinization in buffalo ovary.  Steroid Biochemistry and Molecular Biology 130(1-2):45-56.
7. Sunita,, Singh, P. K., Onteru, S. K., & Singh, D. (2018). Histone deacetylase: A potential therapeutic target for ovarian dysfunction. Frontiers in bioscience (Landmark edition), 23, 512.
8. Sharma, D., Golla, N., Singh, S., Singh, P. K., Singh, D., & Onteru, S. K (2019) An efficient method for extracting next‐generation sequencing quality RNA from liver tissue of recalcitrant animal species. Journal of cellular physiology. Jan 29.
9. Golla, N., Chopra, A., Boya, S., Kumar, T. V. C., Onteru, S. K., & Singh, D. High serum free fatty acids and low leptin levels: Plausible metabolic indicators of negative energy balance in early lactating Murrah buffaloes. Journal of cellular physiology. 2019. Jun;234(6):7725-773
10. Baddela, V. S., Onteru, S. K., & Singh, D. (2017). A syntenic locus on buffalo chromosome 20: novel genomic hotspot for miRNAs involved in follicular-luteal transition. Functional & integrative genomics, 17 (2-3), 321-334.
11. Chaurasiya, V., Kumari, S., Onteru, S. K., & Singh, D. (2020). miR-326 down-regulate CYP19A1 expression and estradiol-17b production in buffalo granulosa cells through CREB and C/EBP-β. The Journal of Steroid Biochemistry and Molecular Biology, 199, 105608.
12. Mehta A, Ravinder, Onteru SK, Singh D (2015) HDAC inhibitor prevents LPS mediated inhibition of CYP19A1 expression and 17β-estradiol production in granulosa cells. Molecular and Cellular Endocrinology, 414, 73-81.
13. Rani, P., Vashisht, M., Golla, N., Shandilya, S., Onteru, S. K., & Singh, D. (2017). Milk miRNAs encapsulated in exosomes are stable to human digestion and permeable to intestinal barrier in vitro. Journal of Functional Foods, 34, 431-439.
14. Chaurasiya V, Kumari S, Onteru SK, Singh D. (2020) Up-regulation of miR-326 regulates pro-inflammatory cytokines targeting TLR-4 in buffalo granulosa cells. Molecular Immunology, 119, 154-158.
15. K Onnureddy, SK Onteru, D Singh (2015) IGF-1 attenuates LPS induced pro-inflammatory cytokines expression in buffalo (Bubalus bubalis) granulosa cells. Molecular immunology 64 (1), 136-143.
16. Sharma I and Singh D (2012) Conjugated linoleic acids attenuate FSH and IGF-1 stimulated cell proliferation, IGF-1, GATA-4, aromatase expression and oestradiol-17β production in buffalo granulosa cells involving PPARγ, PTEN and PI3K/Akt. Reproduction, 144 373–383.
17. Rani, P., Onteru, S. K., & Singh, D. (2020). Genome-wide profiling and analysis of microRNA expression in buffalo milk exosomes. Food Bioscience, 38, 100769.
18. Vashisht, M., Rani, P., Sunita., Kumar Onteru, S., & Singh, D. (2017). Curcumin primed exosomes reverses LPS induced pro inflammatory gene expression in buffalo granulosa cells. J Cell Biochem. 2018 Feb;119 (2):1488-1500.
19. VR Yenuganti, R Ravinder, D Singh (2015) Conjugated Linoleic Acids Attenuate LPS‐Induced Pro‐Inflammatory Gene Expression by Inhibiting the NF‐κB Translocation through PPARγ in Buffalo Granulosa Cells. American Journal of Reproductive Immunology 72 (3), 296-304.
20. Holly A. LaVoie, Dheer Singh and Yyonne Y. Hui (2004) Concerted Regulation of the Porcine steroidogenic Acute Regulatory Protein Gene Promoter Activity by Follicle-Stimulating Hormones and Insulin-Like Growth Factors I in Granulosa cells Involves GATA-4 and CCAAT/Enhancer Binding Protein-beta. Endocrinology, 145 (7) 3122-3134.

Qualifications :

• Ph. D (National Dairy Research Institute, Karnal, India)
• M. S. (National Dairy Research Institute, Karnal, India)
• B. S. (Gujarat Agricultural University, Anand, India)

Major Research Accomplishments :

Awards :

• Best poster paper award for paper titled “Antihyperlipidemic effect of Arjuna Ghee (Herbal Ghee)” presented at the 20th Indian Convention of Food Scientists and Technologists (IFCON) held at NIMHANS Convention Centre, Bengaluru during December 21-23, 2009.
• Recipient of Dr. J. R. Patel Memorial Education and Research Award for the year 2007.
• Third best paper award for paper entitled “Application of response surface methodology for standardization of technology for the manufacture of Kradi cheese using culture NCDC 167” presented in International Conference on Traditional Dairy Foods, held at NDRI, Karnal during November 14-17, 2007
• Best paper award of NDRI for paper entitled Turant taiyar punarvinirman-yogya Basundi mishran ka vikas. Hindi Week, September 14, 2005, NDRI, Karnal.
• Best poster presentation award (2004) for poster entitled “Development of a Process for Ready-to Reconstitute Rasomalai Mix” at the poster session during XXXIII Dairy Industry Conference held at Hotel ‘The Ashok’, New Delhi during 27-28, Sepember, 2004.
• Best research paper award of IDA for paper entitled “Kinetics of deteriorative changes in stored UHT milk – pH and acidity” published in Indian J. Dairy Sci. 53: 123 – 130 (2000).
• ICAR Jr. Research Fellowship: Postgraduate studies (1984-86)
• NDRI Sr. research Fellowship: Doctoral studies (1987-91)

Training :

• Participated in the 2nd overall meeting of the ISEKI_Mundus 2 (ISEKI_Mundus 2 Internationalization and Sustainability of ISEKI_Food Network) and ISEKI_Food 3 (ISEKI_Food 3 – Innovative Developments and Sustainability of ISEKI Food) at Wageningen, The Netherlands as invited partner during June 06-09, 2010
• Participated in the 1st overall meeting of the ISEKI_Mundus 2 (ISEKI_Mundus 2 Internationalization and Sustainability of ISEKI_Food Network) and ISEKI_Food 3 (ISEKI_Food 3 – Innovative Developments and Sustainability of ISEKI Food) at SIK – The Swedish Institute for Food and Biotechnology, Gothenburg, Sweden as invited partner during July 01-03, 2009
• Visited Dublin Institute of Technology, Dublin, Ireland, Universidade Catolica Portuguesa – ESB, Porto, Portugal and Katholieke Hogeschool Sint-Lieven, Gent, Belgium during February 18-May 14, 2008 as Visiting Professor under Erasmus programme of the European Union.
• Visited Srilanka as consultant on Dairy Products Development to Department of Animal Production and Health, Peradeniya, Sri Lanka under the aegis of Sri Lanka Council for Agricultural Research Policy, Colombo, Sri Lanka during December 8 to 22, 2006
• Visited Inst. of Food Process Engg., Technical Univ. of Munich, Freising, Germany to participate in workshop for discussing collaborative project on Membrane Concentrated Milk for UHT Sterilization (Volkswagen Foundation-Funded Collaborative Research Project) during July 01- 14, 1999
• Member of the Standing Committee on Minor components and Characterization of Physical Properties of International Dairy Federation during 2004-07
• Member, Food Safety and Standards Authority of India Scientific Panel on labelling and claims/Advertisements, Government of India
• Organising Secretary, International Conference on Traditional Dairy Foods, November 14-17, 2007 held at NDRI, Karnal (www.dairysociety.org)
• Undergone Training in Recent Advances in Membrane Technology organised under the aegis of Centre of Advanced Studies in Dairy Technology at N.D.R.I., Karnal, India: March 20-April 04, 1996
• Undergone Training in Agricultural Research Management at National Academy of Agricultural Research Management (NAARM), Hyderabad, India: Jan-June 1992

Patents, Technology, Methodology, Genetic Stock, Variety, etc.:

• A process for instant rasmalai mix. G.R.Patil, R.R.B.Singh, A.A.Patel, S.K.Nayak and Sangeeta Mishra (Patent details: 2738/DEL/2006)
• Technology for 5 min ready to reconstitute Kheer mix. A.A Patel, G.R.Patil, R.R.B.Singh, Neeraja Tyagi, Vishal Tripathi and Alok Jha (Patent details: 1076/DEL/2006)
• A formulation and process for ready-to-reconstitute Basundi mix. Prateek Sharma, R.R.B.Singh, G.R.Patil and A.A.Patel. (Patent details: 2226/DEL/2007)
• Low fat oven baked rehydratable Gulabjamun. G.R.Patil, R.R.B.Singh, A.K.Singh, A.A.Patel, Anuradha Singh and Rekha Dahiya (Patent details:788/DEL/2007)
• Development of process for herbal ghee (Arjuna Ghee) Rajani Kant, G.R.Patil, R.R.B.Singh and A.A.Patel (Patent details: 640/DEL/2007)
• A process for long-life milk cake. Anil Kumar, G. R. Patil, R.R.B.Singh and A.A. Patel (Patent details: 641/DEL/2007)
• A process for commercial manufacture of Kradi. H.A.Punoo, G. R. Patil and R.R. B.Singh (Patent details: 2134/DEL/2008)
• Development of process for manufacture of Low-fat spread using Ghee. D. D. Patange, A. A. Patel, D. N. Bhosale, G. R. Patil and R. R. B. Singh.(Patent details: 1796/MUM/2009)

Publications :

• Sharma P, Singh RRB, Singh AK, Patel AA and Patil GR (2009) Sorption isotherms and thermodynamics of water sorption of ready-to-use Basundi mix. Lebensmittel wissenschaft und Technologie 42 (1): 441-445
• Singh RRB, Ruhil AP, Jain, DK, Patel AA and Patil GR (2009) Prediction of sensory quality of UHT milk – A comparison of kinetic and neural network approach. Journal of Food Engineering 92 (2): 146-151
• Ahmed Nadeem, Singh RRB, Singh AK, Patel AA and Patil GR (2008) Effect of maltodextrin addition on moisture sorption properties of Khoa. International Journal of Dairy Technology 61(4): 403-410
• Singh AK, Tiwari S, Singh RRB, Tyagi, RK and Arora S (2008) Optimization of ingredient levels for manufacturing malted milk beverage using response surface methodology. International Journal of Dairy Technology, 61 (2): 192-198
• Jayendra Kumar A, Singh RRB, Patil GR and Patel AA (2006) Kinetics of colour and texture changes in Gulabjamun balls during deep-fat frying. Lebensmittel wissenschaft –und Technologie 39 (7): 827-833
• Singh RRB, Rao KH, Anjaneyulu ASR & Patil GR (2006) Water sorption characteristics of raw goat meat: effect of temperature. Journal of Food Engineering 75(2): 228-236.
• Jayendra Kumar A, Singh RRB, Patil GR and Patel AA (2005) Effect of temperature on moisture desorption isotherm of kheer. Lebensmittel wissenschaft –und Technologie 38 (3): 303-310
• Nayak SK, Makrariya A, Singh RRB, Patel AA, Sindhu JS, Patil GR and Tomar, P (2003) Heat stability and flow behaviour of buffalo milk added with corn starch. Food Hydrocolloids 18: 379-386
• Jha Alok, Patel AA and Singh RRB (2002) Physico-chemical properties of instant Kheer mix. Lait 82:501-513
• Singh RRB, Rao KH, Anjaneyulu ASR & Patil GR (2001) Moisture sorption properties of smoked chicken sausages from spent hen meat. Food Research International 34:143-148