• Dr Subrata K. Das

    Dr Subrata K. Das
    Principal Scientist

    Animal Biotechnology Lab Eastern Regional Station, National Dairy Research Institute Kalyani - 741235, Nadia, West Bengal Cell No. 09433361567

    • Current Research Area
      Assisted Reproduction

    Qualifications:

    • Post Doctoral Research, University of New South Wales, Sydney, Australia
    • Ph.D (Anim.Biotechnology), IVRI, Izatnagar
    • M.Sc (Anim.Biotechnology), NDRI, Karnal
    • B.V.Sc & A.H, BCKV, West Bengal

    Major Research Accomplishments

    • Produced first IVF goat kid in India(Malakar, Das et al., 2008, Ind. J. Dairy Sci. 61 (2):136-140., Malakar, Das et al, 2007, Asian-Aust. J. Anim. Sci. 20(6):842-846).
    • Produced embryos through Somatic Cell Nuclear Transfer (SCNT) in buffalo. (Meena and Das, 2006. Anim. Reprod. Sci.93: 258-267).
    • Established Fibroblast cell culture monolayer for SCNT.(Meena and Das, 2006, Ind. J. Biotechnol. 5: 236-238).
    • Produced embryos through Somatic Cell Nuclear Transfer (SCNT) in goat (Das, S.K. et al., 2003, Small Ruminant Res. 48(3): 217-225).
    • Cryopreservation of oocytes through vitrification in buffalo. (Das et al, 1999. Bubalus bubalis1:75-80., Dhali, Das et al., 2000, Theriogenology, 53: 1295-1303 and Dhali, Das et al., 2000, Anim. Reprod. Sci.63: 159-165).
    • Sexing of preimplantation embryos. (Das et al, 1993. Ind J Anim. Sci. 63(9): 970-972., Das et al, 1994,. Ind.J Dairy Sci. 47(5): 396-400).
    • Produced IVF cattle embryos in modular incubator chamber. (Das et al, 2009, Ind. Vet. J. 87 (8):748-750.
    • In-Vitro Embryo Development using Purified Cattle Oviduct Specific Proteins. Livestock Science. (dx.doi.org/10.1016/j.livsci.2012.12.002).

    Guidance to students:

    M.Sc Student as major advisor – 2
    M.Sc Student as advisor –            7

    Awards:

    • Dr. G.B.Singh Memorial Award by The.Indian Society for the Study on Animal Reproduction (ISSAR)- 1993
    • Rajbhasa Award by National Dairy Research Institute (NDRI), Karnal-1996
    • Biotechnology Overseas Associateship Award 2007-08 by Department of Biotechnology (DBT), Govt. of India – 2009

    Training:

    • Agricultural Research Management Training (46th FCARPM) at National Academy of Agril. Research Management (NAARM), Hyderabad.
    • Application of Molecular genetic techniques for livestock improvement. National Dairy Research Institute, Karnal.
    • Endocrinology of Reproductive disorders in farm animals. Indian Veterinary Research Institute, Izatnagar
    • Embryonic stem cell line establishment. Post Doc Training at Stem Cell Lab, Faculty of Medicine, UNSW, Sydney, Australia.

    Patents, Technology, Methodology, Genetic Stock, Variety, etc.:

    • Produced first IVF kid in India.
    • Produced embryos through Somatic Cell Nuclear Transfer (SCNT) in goat
    • Cryopreserved buffalo oocytes by vitrification.
    • Established Fibroblast cell culture monolayer for SCNT.
    • Produced embryos through Somatic Cell Nuclear Transfer (SCNT) in buffalo
    • Produced IVF cattle embryos in modular incubator chamber.

     

    Publications:

    • Malakar, D., Das, S.K., Mukesh, M., Sodhi, M. and Goswami, S.L. (2007). Production of kids from in vitro fertilized goat embryos and their parentage assessment using microsatellite markers. Asian-Aust. J. Anim. Sci. 20(6): 842-846.
    • Meena, C.R. and Das, S.K*. (2006). Development of water buffalo (Bubalus bubalis) embryos from in vitro matured oocytes reconstructed with fetal skin fibroblast cells as donor nuclei. Anim. Reprod. Sci.93: 258-267.
    • Das, S.K*. and Majumdar, A.C. (2004). Reconstruction of in-vitro matured goat oocytes by using synchronized skin fibroblast cell as donor nuclei. Ind. J. Biotechnology, 3: 435-440.
    • Das, S.K*, Majumdar, A.C. and Taru Sharma, G. (2003). In vitro development of reconstructed oocytes after somatic cell nuclear transfer with fetal fibroblast cells. Small Ruminant Res. 48(3): 217-225.
    • Das, S.K*, Upadhyay, R.C. and Madan, M.L. (1999). Heat stress in Murrah buffalo calves. Livestock Prod. Sci. 61: 71-78.
    • Das, S.K*, Chauhan, M.S., Palta, P. and Tomer, O.S. (1997). Influence of cumulus cells on in-vitro maturation of denuded buffalo (Bubalus bubalis) oocytes. The Vet. Rec. 141(20): 522-523.
    • Das, S.K*, Chauhan, M.S., Palta, P., Katiyar, P.K. and Madan, M.L. (1996). Replacement of fetal bovine serum and FSH with buffalo follicular fluid in in-vitro maturation of buffalo oocytes. Theriogenology, 45(1): 245.
    • Madan, M.L., Das, S.K. Palta, P. (1996). Application of reproductive technology to buffaloes. Anim. Reprod. Sci.42: 299-306.
    • Das, S.K., Goswami, S.L., Ramesha, K.P.and Datta, T.K (1994). A technique for chromosome analysis of preimplantation embryos. Ind. J Dairy Sci. 47(5): 396-400.
    • Das, S.K., Goswami, S.L.and Ramesha, K.P. (1993). Sexing of preimplantation embryos. Ind J Anim. Sci. 63(9): 970-972.
    • Das, S. K*. and Santra, A. (2010). In Vitro development of cattle embryos from in vitro matured and fertilized oocytes in modular incubator chamber. Ind. Vet. J. 87(8):748-750.
    • Das, S.K*., Sharma, A.K., Bhatia, V. and Mohanty, A.K. (2011). Oviductal Secretory proteins as media supplement for In-Vitro Embryo Development in cattle. Reproduction, Fertility and Development, 24(1):164.
    • Sharma, A.K., Mohanty, A.K. and Das, S. K*. (2011). Heparin binding Oviductal Secretory proteins as media supplement for In-Vitro Embryo production in cattle. Reproduction, Fertility and Development, 24(1):188-189.
    • Meena, C.R. and Das, S.K*. (2006). Vitrification of water buffalo (Bubalus bubalis) fetal skin fibroblast cells. Ind. J. Biotechnol. 5: 236-238.
    • Meena, C.R. and Das, S.K*. (2005). In vitro development of reconstructed water buffalo (Bubalus bubalis) oocytes after fetal skin fibroblast cell nuclear transfer. Reproduction, Fertility and Development, 17(2): 248.
    • Das, S.K*. and Majumdar, A.C. (2003). Effect of different electric impulses on development of in-vitro matured goat oocytes reconstructed with fetal fibroblast cells. Ind. J. Anim. Sci. 43(11): 43-47.
    • Dhali, A., Manik, R.S., Das, S.K., Singla, S.K. and Palta, P. (2000). Effect of ethylene glycol concentration and exposure time on post-vitrification survival and in vitro maturation rate of buffalo oocytes. Theriogenology, 53 (1): 253.
    • Dhali, A., Manik, R.S., Das, S.K., Singla, S.K. and Palta, P. (2000). Vitrification of buffalo (Bubalus bubalis) oocytes. Theriogenology, 53: 1295-1303.
    • Dhali, A., Manik, R.S., Das, S.K., Singla, S.K. and Palta, P. (2000). Post-vitrification survival and in vitro maturation rate of buffalo oocytes: effect of ethylene glycol concentration and exposure time. Anim. Reprod. Sci.63: 159-165.
    • Das, S.K*, Manik, R.S.and Tomer, O.S. (1999). Cryopreservation of buffalo oocytes by vitrification. Bubalus bubalis, 1:75-80.
    • Das, S.K*, Chauhan, M.S., Palta, P.and Tomer, O.S. (1999). Chromosome configuration of buffalo oocytes matured in vitro. Ind. J. Anim. Sci.69 (10): 803-805.
    • Das, S.K*, Upadhyay, R.C. and Madan, M.L. (1997). Changes in skin temperature and physiological reactions in Murrah buffalo during solar exposure in summer. Asian-Aust. J. Anim. Sci.10 (5):478-483.
    • Chauhan, M.S., Palta, P., Das, S.K., Katiyar, P.K. and Madan, M.L. (1997). Replacement of serum and hormone additives with follicular fluid in the IVM medium: Effects on maturation, fertilization and subsequent development of buffalo oocytes. Theriogenology, 48(3): 461-469.
    • Das, S.K*, Upadhyay, R.C. and Madan, M.L. (1997). Surface and core temperature changes in Murrah buffaloes during cold weather conditions. Ind. J Anim. Sci. 67(7): 581-584.
    • Das, S. K*. and Santra, A. (2010). In Vitro development of cattle embryos from in vitro matured and fertilized oocytes in modular incubator chamber. Ind. Vet. J. 87(8):748-750.
    • Pradeep, M.A., Jagadeesh, J., De, A.K., Kaushik, J.K., Malakar, D., S. Kumar, Dang, A.K., Das, S.K. and Mohanty, A.K. (2011). Purification, sequence characterization and effect of goat oviduct-specific glycoprotein on in-vitro embryo development. Theriogenology. 75(6):1005-15.
    • Jagadeesh, J., Singh, S., Choudhary, S., Pradeep, M.A., Sudarshan, K., Kumaresan, A., Das, S.K. Kaushik, J.K., and Mohanty, A.K. (2012). Molecular cloning, Sequence Characterization and Heterologous Expression of Buffalo (Bubalus bubalis) Oviduct-specific Glycoprotein in E.coli. Molecular Biology Reports.39:10031-10043. DOI 10.1007/s11033-012-1872-9.
    • Das, S. K*, Sharma, A. K., Sushil K. Mahapatra., Chatterjee, A., Bhatia, V. and Mohanty, A. K. (2013). Purification of Cattle Oviduct Specific Proteins and their Effect on In-Vitro Embryo Development. Livestock Science, 152:88-93. (dx.doi.org/10.1016/j.livsci.2012.12.002).
    • Sharma, A.K., Mahapatra, Sushil. K., Mohanty, A.K. and Das, S.K*. (2013). Purification of heparin binding oviduct specific proteins and its effect on in vitro embryo development in cattle. Ind. J. Expt. Biol. 51(5):347-351.
    • Singh, Surya Bansi and Das, Subrata K.* (2014). Embryo development through in vitro maturation and fertilization of cattle oocytes. Nature and Science. 12(8):67-70.(ISSN:1545-0740)
    • Mohapatra, S. K., Singh, S., Kumar, S., Dang, A. K., Datta, T. K., Das, S. K., Mohanty, T. K., Kaushik, J. K. and Mohanty, A. K.* (2014). Comparative expression profiling of insulin-like growth factor binding protein-5 (IGFBP-5) in milk of Bos indicus and Bubalus bubalis during lactation. Animal. Dec 10:1-7.
    • Sharma, Aditya K., Mahapatra, Sushil. K., Mohanty, A.K. and Das, Subrata K*. (2014). Effect of heparin-binding oviduct specific proteins on in vitro embryo development in cattle. Ind. J. Biotechnol. 13(3):408-410
    • Singh, Surya Bansi and Das, Subrata K.* (2014). Cryopreservation of fetal skin fibroblast cells in cattle. Int. J. Biotechnol. & Bioeng. Res. 5 (1):53-57. (ISSN 2231-1238).