Dr Naresh Kumar
Dr Naresh Kumar
Principal Scientist & Nodal Officer – National Referral Center
Dairy Microbiology Division, ICAR-NDRI, Karnal
Email: firstname.lastname@example.org, email@example.com
Current Research Area
Food Safety & Quality Assurance with specialization in the field of biosensor development for rapid detection of Antibiotics, pesticides, Heavy metals, and bacterial pathogens for application in the Dairy industry
- Master Degree (Dairy Bacteriology)
- Doctorate Degree (Dairy Microbiology)
Major Research Accomplishments
One of the significant achievement was to established state of the art infrastructure facility as National Referral Center on Milk Quality and Safety (NRCMQS) which includes BSL-3 Containment under World bank funded NAIP project with fund support of 17.5 Crores in 2014 for addressing the scientific issues on milk safety in our country with the mandate to provide scientific solution in specialized field of milk safety and quality assurance; to carry out Risk Profile Work on Potential Contaminants in milk and milk products; development of milk safety standards, sampling plan and testing procedures for regulatory compliance; Rapid Biosensor Based Tools/Assay/Kits for field application and to conduct training on HRD development on milk quality.
With the established facility at NRC rapid sensors were developed for detection of potential contaminants in dairy food chain by exploring bacterial spores as biosensor.Bacterial Spore as innovative approach for its application as bio-recognition element was successfully employed in rapid detection of antibiotic, pesticides and bacterial contaminants in dairy foods and their commercialization under field condition. Spore-based sensing technologies were developed to target many emerging contaminants in different types of food matrices, including milk and milk products in order to ensure food safety and quality. Different types of approaches were attempted for the development of spore-based biosensors for detection of antibiotic, pesticides and bacterial contaminants in milk.
Paper Strip Based concept for Detection of Antibiotic Residues in Milk:
Paper-based sensors are new alternative technology for fabricating simple, low-cost, portable and disposable analytical devices for many applications. The major advantages of using paper as a sensing platform include ease of availability, low cost, passive liquid transport, compatibility with chemicals/bio-chemicals and fast response. With these basic properties in mind, a spore-germination inhibition based assay for antibiotic detection was developed on the paper strip for exploring its potential under field application. The stages involved in developing paper sensors include the proper choice of paper, fabrication/patterning, and semi- quantitative analysis based on color development as a result of specific marker enzymes released during spore germination.The test involves pre-dipping of strip in milk and germination of spores in presence of germinant mixture when incubated at 64o C for 1:15 hrs. Development of blue color on paper strip indicates the absence of antibiotic residues and no color development indicates presence of antibiotic Residues in milk. The technology was developed under externally funded Outreach project on monitoring of drug residues and environmental pollutants funded by ICAR with fund support of 71 lakhs.
Spore Based Kit for Detection of Antibiotic Residues in Milk at Dairy Farm: The developed technology is working on principle of spore germination and its inhibition in presence of antibiotic residues in milk. In case when antibiotic residues are absent in milk, marker metabolites are released during germination which change the color of the indicator. However, in presence of antibiotic residues in milk, the spore germination process is inhibited at ≥ MRL level of contaminants and no change in color indicates the presenceofdrugresiduesinmilkwhenincubatedat 64o C for 2.30 hrs.
Technology was developed under externally funded Ministry of Food processing (MFPI) with fund support of 41 lakhs.
Paper Strip based concept for Rapid Detection of Pesticide Residues: Pesticides are well known carcinogen and their impact on human beings and presence in different food products including milk are well known in the prior art. The existing conventional chromatographic methods (LC/GC-MS) are time- consuming and laborious. Currently, new standards for pesticides have been developed by FSSAI and implemented for regulatory compliance in different food products including milk. For routine monitoring of pesticides under field application, three stage assay on paper strip has been developed based on “spore germination and enzymeinhibition principle”. In case where analyte i.e. pesticide is absent, specific marker enzyme (s) are produced by spores during germination which will act specifically on chromogenic substrate resulting in colored end product on paper strip, whereas complete inhibition of marker enzyme will take place when pesticides are present in food sample (Patent Reg. 3819/DEL/2015).Simple and cost- effective technology for field application for testing of pesticides residues in milk, cereal based foods, fruit juices, feed, fodder, soil, water, manure etc. Technology was developed under externally funded Niche area of excellence (NAE) with funded by Education Division ICAR with support of 121.420 lakhs
Spore Based concept on Biochip for Listeria monocytogenes, Enterococci and E. coli in milk: A miniaturized spore based assay for detection of bacterial contaminants in milk on functionalized gold chip with minimal quantity of milk and other reagents with enhanced sensitivity was developed. The developed assay was integration with other consortia partners to have multi-analytehigh throughout put analysis for dairy applications. The spore enzyme assay was evaluated on biochip using EMCCD camera. The fluorescent signals were quantified in terms of integrated optical density (IOD).Patent on the technology has been published in IPO (1357/ DEL /2013) in Indian patent journal no. 50/2014, Dated12/12/2014. Technology was developed under externally funded NAIP project funded by World bank with support of 22.0 crores
- Fellow of National Academy of Dairy Science (NADSI) -2014
- Fellow of National Academy of Agriculture Science (FNAAS)- 2019
- Eminent Scientist award conferred by the Samagra Vikas Welfare Society (SVWS) for outstanding contribution in the field of Agriculture. (2017)
- Recognition by “Food and Agricultural Organization of the United Nations (FAO) 2017
- Recognition by “CG Centre -international live stock research Centre (ILRI)-2017
- Recognition/ Appreciation from “World Bank team members during their visit at NDRI while evaluating the performance of NATIONAL REFERRAL CENTRE FOR MILK QUALITY AND SAFETY established under NAIP project -2014
- Best consortia award in NAIP project on development of biosensors and micro-techniques for bacterial contaminants in milk (2010)
- Nominated as part of Indian delegation for attending codex meeting on drug residues held at Minneapolis, Minnesota (2013)
- Scientific panel members on bio-hazard in FSSAI and contributed in development of regulatory microbiological standards for dairy industry (2008 onwards)
- Recognition by Board of Management (BOM) of NDRI (2008)
- Recognition by “Centre for Science (CSE) and Environment, New Delhi
- Recognition by ICAR-NDRI by Nominated as member of the Board of Management (BOM) of ICAR-NDRI by the director for two years (2018-19)
- Appointed as Member secretary of codex committee by the Director w.e.f. 2013 onwards
- Nominated as Expert member of Institute Bio-safety Committee (IBSC) by Director of the institute. (2015-19)
- Training program on E-learning for dairy science faculty under NAIP project at National academy of agricultural research management , Hyderabad for 10 days i.e. October 19-29 , 2010
- One week training programme on AMR under ICAR-ILRI project Organized by ILRI under ICAR-ILRI project entitled “Assessment of Antimicrobial Residues and Resistance in Dairy Animals in India at NDRI from (3rd Jan. 2017 – 9th Jan. 2017)
- Training on Laboratory Quality Management Systemand internal audit as per IS/ISO/IEC 17025 :2005 organized by National Institute Of Training For Standardization (NITS) for NABL accreditation at Bureau Of Indian Standard (BIS) ,Noida from (20-23 Feb. 2018)
- Training Programme on procurement related matters and financial management system Participated in National Training Programme on procurement related matters and financial management system under world bank funded project sponsored by PIU-NAIP –ICAR from 3-4 February 2009.
- Training on Quality Management system (ISO: 9001:2000) and Food Safety Management System (HACCP)
- Novel Process of Sporulation, Activation and Germination in Thermophilic Bacteria for Rapid Detection of Antibiotic Residues in Milk. Patent grant No: 264145 dated 9.12.2014.Inventors: Kumar Naresh, Patil G. R., Rane S., Malik R. K. (2014).
- Development of spore inhibition based enzyme substrate assay(SIB-ESA) for monitoring aflatoxin M1 inmilk Patent grant no: Indian Patent No. 292836 dated 13/02/2018. Inventors: Kumar, Naresh, Singh, N.A., Singh. K., Malik, R. K. and Raghu, H.V. Sunial Bhand (2010).
- Technology on Spore Germination Based Detection Kit For β-Lactam Group in Milk. Patent grant no: 273160 granted on 20/05/2016 Inventors: Naresh Kumar, Sougata Das and Manju Gare.
- Novel enzyme-substrate based bio-assay for real time detection of monocytogenes” in milk system. Patentpublicationno1357/DEL/2013-Published in Indian patent journal no. 50/2014, Dated12/12/2014.Inventors:Balhara, M., Kumar, N., Thakur, G., Raghu, H. V., Singh, V. K., Lawania, R., Khan, A., Shabnam (2012).
- Real time detection of enterococci in dairy foods using spore germination basedbioassay. Patent publication no: 119/DEL/2012 published in patent journal no.– 19/2015 dated 08/05/2015 Inventors: Kumar Naresh, Kaur G, Thakur G, Raghu HV, Singh N, Singh VK and Raghav N. (2012).
- Rapid spore enzyme based miniaturized assay (s) for detection of pesticide residues. Patent publication no: Indian Patent Reg. No. 3819/ DEL/ 2015. Publication journal no. 21/2017 dated 26 May 2017 Inventors:NareshKumar,N.Tehri,R.Gopaul,P. K.Sharma, S.Morab and Raghu H V.(2015).
- Development of enzyme-spore based assay (s) for monitoring antibiotic residuesin milk. Patent publication no: 2213 /DEL/2014 Publication Journal no. 07/2016, U/S 11A dated 2016/02/12. Inventors : Naresh Kumar, A. Khan, S. Arora, F. Patra, M. Dahiya, Raghu H.V., M. Balhara, P. Kumar, S. Shaikh. (2014).
- Marker enzymes and spore germination based assay for detection of E. coli in milk and milk products. Indian Patent no. 2214/DEL/2014.Inventors : Naresh Kumar, Avinash , Ramakant, Raghu H.V., (2014)
Technology Developed, validated and Licensed:
- Technology “Detection of L. monocytogenes in milk”
- Validation: M/s. SGS India Pvt.Ltd. Gurgaon dated 9th Nov 2012
- Commercialized to: M/s Neugen Diagnostics (India) Pvt. Ltd., Hyderabad, with an amount of 5 Lakhs as non-exclusive license during Agri-Tech Meet organized at NASC complex dated 18-19th July 2013
- Technology “Detection of Enterococci in milk”
- Validation: M/s. SGS India Pvt.Ltd. Gurgaon dated 9th Nov 2012
- Commercialized to: M/s Neugen Diagnostics (India) Pvt. Ltd. Hyderabad, with an amount of 5 Lakhs as non-exclusive license during Agri-Tech Meet organized at NASC complex dated 18-19th July 2013.
- Technology “Paper strip assay for rapid detection of pesticide residues in milk”
- Validation:Validated byNABL accredited SGS laboratory, Gurgaon. The results obtained with developed assay was compared withGC-MS/MS, LC-MS and similar results were obtained.The limit of detection (LODs) as claimed were found reproducible during validation work. No false positive or negative results were observed. Certificate no ref no gg17-001563.001 dated 20-4-2017
- commercialized to :
- M/s Hatsun Agro product limited Agro, Chennai with an amount of 5.75Lakh as non-exclusive license, during Institute-Industry Meet, 17th Dec 2016.
- M/s. Florecer Services Private Limited, New Delhi, through Agrinnovate with nonexclusive license fee of Rs.5.90 Lakhs+ 2% Royalty dated 01 July 2019
- Technology “Spore based kit for detection of antibiotic residues in milk at dairy farm”,
- Validation: validated by third party throughanopentenderandM/s.DoveResearchand Analytics, Panchkula (A unit of Dove Chemicals)intermsof working test procedure, sensitivity against various groups of antibiotics as per Limits of Detection (LODs). Certificate noDRA/NDRI/16 17/080217/001dated9-02-2017.
- M/s Hatsun Agro Private Ltd. Chennai with an amount of 3.45 Lakh as non-exclusive license during Institute-Industry Meet, 17th Dec 2016.
- M/s. Delmos Research Pvt. Ltd. Gurgaon with an amount of 3.54 Lakh as non-exclusive license on 12 March 2019.
- Technology“Paper strip for detection of antibiotic residues in milk”
- Validation: validated by third party through an open tender and M/s. Dove Research and Analytics, Panchkula(AunitofDoveChemicals)in terms of working test procedure, sensitivity against various groups of antibiotics as per Limits of Detection (LODs). Certificate no DRA/NDRI/16-17/080217 /002 dated 9-2-2017
- M/s. Florecer Services Private Limited, New Delhi, with an amount of Rs. 5.90 Lakh as non-exclusive license dated 01 July 2019.
- Kumar, N*., Raghu, H. , Kumar, A., Haldar, L., Khan, A., Rane, S., &Malik, R. K. (2012). Spore germination based assay for monitoring antibiotic residues in milk at dairy farm. World Journal ofMicrobiology &Biotechnology, 28 (7), 2559-2566
- Tehri,N.*, Kumar, N., Raghu, H. V., Thakur,G., Sharma, P.K. (2017). Role of stereospecific nature of germinant in Bacillus megaterium spores 3 Biotech.7:259(1 10).DOI 10.1007/s13205-017-0897-0
- Das, S., Kumar, N*, Vishweswaraiah, R. H., Haldar, L., Gaare, M., Singh, V. K., &Puniya, A.K. (2014). Microbial based assay for specific detection of β-lactam group of antibiotics in milk. Journalof food science technology,51(6),1161-1166
- Singh,N.A.,KumarN*.,V. Raghu, P. K.Sharma,K. Singh, Alia Khan,and N. Raghav (2013) “Spore inhibition based enzyme substrate assay for monitoring of Aflatoxin M1 in milk. Toxicological &Environmental Chemistry. 95 (5): 755-777 DOI.org/10.1080/0277 2248.2013.807540
- Kaur, G., Malik, R. K., Mishra, S. K., Singh, T. P., Bhardwaj, A., Singroha, G. and Kumar, N. (2011). Nisin and class IIa bacteriocin resistance among Listeria and other foodborne pathogens and spoilage bacteria. Microbial drug resistance, 17(2),197-205
- Bhardwaj, A., Gupta, H., Iyer, R., Kumar, N., and Malik, R. K.* (2009). Tyramine-producing enterococci are equally detected on tyramine production medium, by quantification of tyramine by HPLC, or by tdc genetargeted PCR. Dairy Science and Technology, 89(6), 601-611
- Singh, V. K., Singh, N. A., Kumar, N., Raghu, H. V.*, Sharma, P. K., Singh, K. P., & Yadav, A. (2014). Spore immobilization and its analytical performance for monitoring of aflatoxin M1 in milk. Canadian journal of microbiology, 60(12), 793-798DOI1139/cjm- 2014-0465
- Kumar, S.*, Srivastava, A. K., Dumka, V. K., Kumar, N., and Raina, R. K. (2010). Plasma pharmacokinetics and milk levels of ceftriaxone following single intravenous administration in healthy and endometritic cows. Veterinary research communications,34(6), 503-510
- Singh, V.K., Singh, N.A., Raghu, H.V., Kumar, N*, Singh, K.P., Sharma, P.K. and Raghav, N. (2016), Fluorescence-Based Detection of Aflatoxin M1 in Milk using Immobilized Spores. Journal of Food Safety, 36: 145–152. doi:10.1111/jfs.12221
- Kumar, S.*, Srivastava, A. K., Dumka,V. , &Kumar, N.(2014). Intramuscular Pharmacokinetics and Milk Levels of Ceftriaxone in EndometriticCows. Israel Journal of Veterinary Medicine, 69(3), 130-135
- Kumar, S., H. Raghu,Kumar, N*. Singh, and R. K. Malik. (2013). Spore based chromogenic assay for detection of β-lactam antibiotic in milk. Indian Journal of Dairy Science 66(6):504-517
- Sharma, P.K*., Sharma, P.K., Kumar, N., Suman and Dhingra, N. (2017).Identification and Characterization of BileSalt Hydrolyzing Lactobacillus International Journal of Current Microbiology and Applied Sciences 6(3): 1655-1675
- Sudarshan Kumar*; A Bhardwaj; Srivastava, A.; Mutha Rao; Naresh Kumar(2015). White side test – a field test on the cervical mucus of cows for diagnosis of endometritis. Intas Polivet 16(2):207-213
- R Lawaniya*, M Balhara, A Khan, B Arora, N Kumar, Avinash, P K Sharma, H V Raghu and R K Malik (2015). Screening of commercial enrichment media for selective growth of coli. Indian J. Dairy Science, 68(1)
- Mandeep Balhara*,R.Lawaniya, Naresh Kumar, H. Raghu, ShabnamKouser, Pradip Kumar Sharma and Malik R.K (2014). Assessment ofenrichment broths for their ability to recover Listeria monocytogenes from spiked milk. Indian J. Dairy Science, 67(6)
- Nimisha Tehri, Naresh Kumar, Avinash Yadav, Raghu H.V., Namita Ashish Singh (2018). Sugars mediated germination in spores of Bacillus megaterium. International Journal of Microbiology Research: 10 (3), 1058-1061, 2018
- Singh, N. A., Kumar, N., Raghu, H. V., Bhand, S., Chandra, S., & Sharma, P. K. (2018). A spore-based miniaturized novel assay for rapid aflatoxin detection in milk. Environmental Chemistry Letters 17(2), 1097-1103, 2019
- Tehri, N., Kumar, N., Raghu, H. V., &Vashishth, A. (2018). Biomarkers of bacterial spore germination. Annals of microbiology 68(9), 513-523
- Thakur G., Yadav A., Tehri N., Kumar N*. Raghu H.V., Singh N. and Singh.K. (2013) Rapid &novel microscopy technique to detect germinationinitiation and specificity in Bacillus International Journal of Research in Pure and Applied Microbiology,3(4): 134-138
- Yadav K., Bhardwaj A., Kaur G., Iyer R., De S., Kumar N., and Malik R. K. (2009). Potential of Lactococcus lactis as a Probiotic and Functional Lactic Acid Bacteria in Dairy Industry. International Journalof Probiotics and Prebiotics, 4(3):219-228.
- Verma N, Singh NA, Kumar N, Singh VK, Raghu HV (2013) Development of “Field Level” Chromogenic Assay for Aflatoxin M1 Detection inMilk. Adv Dairy Res 1: 108. doi: 10.4172/2329- 888X.1000108/ (2013) : 2-8
- Kumar N, Thakur G, Raghu HV, Singh N, Sharma PK, (2013) Bacterial Spore Based Biosensor for Detection of Contaminants inMilk. J Food Processing Technology4: doi:10.4172/2157- 7110.1000277
- Shivani Arora1*, Naresh Kumar, Avinash Yadav1, Hirikyathanahalli Vishweswaraiah Raghu1 (2016) Spore: potential of invaluable bacterial wrap. Int. J. Life. Sci. Scienti. Res., 2(5): 513-518
- Geetika Thakur, Raghu H.V1 2 ., Tehri N., Kumar N., Avinash Y., and Malik R. K (2014) Biochip based detection- An emerging tool for ensuring safe milk. Journal of Innovative Biology, 1, 147-154
- Tehri N., Kumar N., Vashishth A. (2019) Current Trends in enzymatic biosensors for pesticides determination (accepted)
- Gaare, M., Kumar, H.V. Raghu*, A. Khan, and V.K. Singh. 2012. “Specific Detection of β-lactam AntibioticsinMilkbySpore BasedAssay.” International Research Journal of microbiology 3: 168–173
- NeelamVerma, Namita Ashish Singh*, Kumar N. and H. V. Raghu (2013). Screening of different media for sporulation of International Journal of Microbiology Research &Reviews. 1 (4):68-73
- H Raghu, N Kumar, K Mayuri Arya, P Sharma (2017)Screening of Lectins for Specific Detection of Listeria Monocytogenes. Journal of Microbiology & Experimentation, volume 5 (3): 00150. DOI: 10.15406/jmen.2017.05.00150
- K. Sharma, P. K. Sharma, N. Kumar, and M. Kumar. (Molecular identification of bile tolerant Lactobacillus fermentum strains isolated from Human Faeces. International Journal of Agricultural Invention 1(2):182-189: Dec, 2016
- Naresh Kumar* and Prasad, D.N. (1992). Inhibitory effect of nisin on various Lactic and non-lactic microorganisms. Microbiologie-aliments-nutrition. 10:359-363
- Naresh Kumar* and Prasad, D.N. (1992). Comparative Nisin sensitivity of Lactococcus spp in broth and skimmed milk under optimum growth conditions. Microbiologie-aliments-nutrition. 10:181:184
- Naresh Kumar* and Prasad, D.N. (1993). Factors affecting the inhibitory action of nisin against Lactococcus and streptococcus spp. Microbiologie-aliments-nutrition. 11:269-275
- Naresh Kumar* and Prasad, D.N. (1994). Scanning electron microscopy studies of nisin action against gram positive bacteria. Microbiologie-aliments-nutrition. 112:429-436.
- Naresh Kumar* and Prasad, D.N. (1994). Factors affecting the inhibitory action of nisin against Lactobacillus and bacillus spp. Microbiologie-aliments-nutrition. 12:155-161
- Naresh Kumar* and Prasad, D.N. (1994). Effect of incubation period on inhibitory action of nisin in skimmed milk against lactic and non-lactic microorganisms. Microbiologie-aliments-nutrition. 12:163-167.
- Manual of Good Practices and safety for National Referral center for milk quality and safety. Volume –II, National Dairy Research Institute, Karnal, India. Naresh Kumar, Rajan Sharma, A. K. Mohanty and Raghu H V. (2014) Published and release by Agriculture minister during ICAR foundation day Pp 1-289.
- A Biosafety manual –A holistic approach biosafety in dairy industry. Naresh Kumar, Raghu H V., RK Malik, Geetika Thakur, Mandeep, Ramakant. (2012) NDRI Publication under NAIP Funded projectMicrobiology of Processed Dairy Foods” complied by and published by Dairy Microbiology Division, NDRI, Karnal. Malik, R. K., vij, Shilpa Kumar N. March 2009 (NDRI publication no 44/2009)
- A manual on Microbiological Quality and Safety Evaluation of Dairy Products. Raghu H V., Kumar N. and Malik R. K. (2014) NDRI Publication No. 124/2014. Pp 1-185
- Training programme on fundamental aspects of dairy microbes and their safety concerns in dairy industry. Naresh Kumar, R K Malik, S K Tomar, Rameshwar Singh (2009) NDRI Publication
- Conventional and rapid techniques for monitoring quality and safety in dairy industry. Naresh Kumar, R K Malik, S K Tomar, Rameshwar Singh (2010) NDRI Publication
- Conventional and Rapid Microbiological Techniques for Quality and Safety Monitoring in Dairy Industry. Naresh Kumar and Raghu HV (2011) NDRI Publication
- Microbiological and chemical analysis of milk and milk products. Raghu HV, Naresh Kumar and Rajan Sharma (2017) NDRI Publication
- Microbial Analytical technique for Quality assurance of milk and milk products. Naresh Kumar, Raghu HV (2014) NDRI Publication
- Current Quality Concepts for Ensuring Safety of Live Stock Products. Naresh Kumar and Rajan Sharma (2013) NDRI Publication
- Chemical and microbiological analysis of milk and indigenous milk products. Dr Sumit Arora, Dr. Vivek Sharma, Dr. Naresh Kumar& Dr Rajesh Kumar (2009) NDRI publication
- Analytical Potential of Bacterial Spores for Assessment of Milk Quality in “Microbial Cell Factories” Nimisha Tehri, Naresh Kumar, Rajesh Gopaul,Pradip K Sharma, and H. V. Raghu (2018) published by Taylor and Francis Group, CRC Press
- Microbial Spores: Concepts and Industrial Applications in “Microbial Bio-prospecting for Sustainable Development. Nimisha Tehri, Naresh Kumar,V. Raghu, Ravi Shukla, Amit Vashishth (2018) published by Springer 978-981-13-0053-0
- Monitoring of veterinary drug residues in milk and milk products- present scenario in India in Food safety, quality assurance, global trade: concept and strategies. Kumar N. (2009) Published by Dr S P Singh ,Julie Funk ,S C Tripathi And Nanda Joshi in International Book Distributing Co. publication division ISBN 8189-268-2
- Analysis of quality of drinking water in “Microbiology-an overview. Singh N.A., Kumar, N., H.V. Raghu. (2016) published by IK International Publishers, New Delhi (In Press)
- Prospects of organic dairy farming in India and the performance behavior and milk quality of organically managed buffaloes in Global organic agribusiness India.-Arrives. Kamboj M. L., Harika A. S., Chhabra A., Prasad S. and Kumar N. (2008) published by Menon M K., PaulY. S., Muralidhara, N
- Estimation of Antibiotic Residues in Milk in Handbook of Milk Analysis. Kumar N. and Malik R. Edited by K. Anjali Aggarwal, Veena Mani. Harjit Kaur Pg. 57-65 (March 2008)D.R.I. Publication no. 38/2008.
- Functional Foods: Their Role in Disease Prevention and Health promotion in Applied Dairy and Food Microbiology –contemporary reviews. Malik R. K. and Kumar N. Ed by Rameshwar Singh, S. K. Tomar and Gunjan Goel (Eds) (2005 Reprinted in 2008)D.R.I. Publication