Dr. Manoj Kumar Singh
Animal Biotechnology Centre, ICAR-NDRI, Karnal
Current Research Area
Embryo Biotechnology, Somatic Cell Cloning, Stem Cell Biology, Transgenesis, Ovum pick-up.
|Degree||Name of the College/Institute and University||Passing Year|
|M.V.Sc. & A.H.
|Biotechnology Centre, J.N.K.V.V., Jabalpur||2004|
|B.V.Sc. & A.H.||Bombay Veterinary College, Mumbai/M.A.F.S.U., Nagpur||2002|
Research Projects handled/being handled:
|Title of the project||Level of association (PI/Co-PI)||Period||Sponsoring organization
|Project Cost in Lakhs|
|1.||Conservation of Indigenous Pig of Assam through Handmade Cloning technique||PI
|02.01.2017||02.07.2020||DBT, New Delhi||40.59 Lakh
|2.||Global transcriptome and miRNA analysis for deciphering reasons for low cloning efficiency in buffalo||Co-PI||22.03.2018||21.03.2021||DST, New Delhi
|3.||Production of multiple copies of elite buffalo bulls using animal cloning technology||Co-PI||01.04.2018||31.03.2022||NASF, New Delhi||264.3 Lakh|
|4.||Synthetic Endometrium: A novel model to study early embryonic development and uterine health in ruminants||Co-PI||01.04.2017||31.03.2020||NASF (ICAR)||77.48 Lakhs
|5.||Generation of induced pluripotent stem (iPS) cell like cells from buffalo fibroblasts||PI||01.04.2014||31.03.2017||N.D.R.I., Karnal (Institute Project)||27.00 Lakh|
|6.||Development of parthenogenetic goat from embryonic stem cells||PI
|01.04.2013||31.03.2017||NFBSFARA (ICAR)||90.84 Lakhs|
|7.||Semen sexing in cattle
|Co-PI||June 2015||31.03.2017||ICAR, New Delhi|
|8.||Expression of apoptosis-related genes in buffalo embryos produced in vitro under different oxygen concentrations.||PI||January, 2010||Dec, 2012||N.D.R.I., Karnal
|9.||Establishment of facilities for research and training in stem cell technology in pigs.||Co-PI
PI from March 30, 2016
|April, 2012||October, 2016||DBT, New Delhi||37.38 Lakhs|
|10.||Buffalo transgenesis for expression of desired proteins.||Co-PI
PI from March 30, 2016
|June, 2014||June, 2016||DBT, New Delhi||59.078 Lakhs|
|11.||Development of transgenic goat for production of human lactoferrin.||(Co-PI)
PI from March 30, 2016
|June, 2012||May, 2016||NFBSFARA (ICAR)||253.68 Lakhs|
|12.||Characterization and differentiation of embryonic and spermatogonial stem cells in cattle and buffaloes.||Co-PI||Sep. 2008 Joined from July, 2009||June, 2014||N.A.I.P., New Delhi||398.51 Lakhs|
|13.||Development of transgenes for in vitro expression in buffalo mammary gland epithelial cells.||Co-PI||Sep. 2011||Sep. 2013||DBT, New Delhi||43.08 Lakhs|
|14.||Improvement in fertilizability of cryopreserved buffalo bull semen by minimizing cryocapacitation and apoptosis like changes.||Co-PI||March, 2011||Feb, 2013||DBT, New Delhi||17 Lakhs
|15.||Molecular cloning and expression of buffalo leukemia inhibitory factor (LIF) in eukaryotic system.||Co-PI||January, 2010||Dec, 2012||N.D.R.I., Karnal
|16.||Embryo production by Ovum Pick-Up from live cattle.||Co-PI||Sep. 2008 Joined from July, 2009||August, 2011||DBT, New Delhi||Rs 58.88 Lakh|
|S. No.||Course code||Course nomenclature||Credit hours|
|1.||BT – 613||Immunology Applied to Biotechnology||(2+1)|
|2.||BT – 614||Reproductive Biotechnology||(2+1)|
|3.||BT – 713||Advances in Reproductive Biotechnology||(3+0)|
|4.||BT – 722||Advances in Animal Cell Culture||(3+0)|
|5.||LP-411||Anatomy of Farm Animals||(2+1)|
|6.||GS – 635||Disaster Management||(1+0)|
- Publications: 46
|Articles in Foreign Referred Journals (39)|
|1.||Propagation of goat putative spermatogonial stem cells under growth factors defined serum-free culture conditions.||Sharma A, Shah SM, Tiwari M, Roshan M, SinghMK, Singla SK, Palta P, Manik RS and Chauhan MS.||2020 Cytotechnology, 72:489-497.|
|2.||Successful transplantation of transfected enriched buffalo (Bubalus bubalis) spermatogonial stem cells to homologous recipients.||A. Sharma, A. Kumaresan, P. Mehta, N. Nala, M.K. Singh, P. Palta, S.K. Singla, R.S. Manik and M.S. Chauhan.||2020
Theriogenology, 142: 441-449.
|3.||Media switching at different time periods affects the reprogramming efficiency of buffalo fetal fibroblasts.||N. Rawat, M.K. Singh, T. Sharma P. Vats, D. Nagoorvali, P. Paltaa, M.S. Chauhan, and R.S. Manik.
Animal Biotechnology, DOI: 10.1080/10495398.2019.1671435
|4.||Selection of Reference miRNAs for Relative Quantification in Buffalo (Bubalus bubalis) Blastocysts Produced by Hand-Made Cloning and In Vitro Fertilization.||S.V. Lagah, T.J. Sood, P. Palta, M. Mukesh, M.S. Chauhan, R.S. Manik, M.K. Singh and S.K. Singla.
Cellular Reprogramming, 21(4): 200-209.
|5.||Calcium ionophore enhanced developmental competence and apoptotic dynamics of goat parthenogenetic embryos produced in vitro.||Dua D., Nagoorvali D., Chauhan M.S., Palta P., Mathur P., Singh M.K.||2019
In Vitro Cell Dev Biol Anim. 55(3):159-168
|6.||Comparative analysis of buffalo (Bubalus bubalis) non-transgenic and transgenic embryos containing human insulin gene, produced by SCNT.||P. Mehta, R. Kaushik, K.P. Singh, A. Sharma, M.K. Singh, M.S. Chauhan, P. Palta, S.K. Singla, R.S. Manik.||2019
Theriogenology 135 25e32
|7.||Supplementation of glial cell line-derived neurotrophic factor, fibroblast growth factor 2, and epidermal growth factor promotes self-renewal of putative buffalo (Bubalus bubalis) spermatogonial stem cells by upregulating the expression of mir-20b, mir-21, and mir-106a.||A. Sharma, S.V. Lagah, D. Nagoorvali, B.S.B. Kumar, M.K.Singh, S.K. Singla, R.S.Manik, P. Palta,and M.S. Chauhan.||2019
Cell Reprogram. 2019Feb;21(1):11-17.
|8.||Optimization of serum-free culture conditions for propagation of putative buffalo (Bubalus bubalis) spermatogonial stem cells.||A. Sharma, S.M. Shah, N. Saini,P. Mehta, B.S.B. Kumar, D. Dua, M.K. Singh, S.K.Singla, P. Palta, R.S. Manik and M.S. Chauhan.||2019
Cell Reprogram. 2019 21(1):1-10.
|9.||Treatment of buffalo (Bubalus bubalis) SCNT embryos with microRNA-21 mimic improves their quality and alters gene expression but does not affect their developmental competence.||Rashmi, S. Sah, S. Shyam, M.K. Singh, P. Palta.||2019
Theriogenology 126, 8-16
|10.||Epigenetic alteration of donor cells with histone deacetylase inhibitor m- carboxycinnamic acid bishydroxymide (CBHA) improves the in vitro developmental competence of buffalo (Bubalus bubalis) cloned embryos.||Agarwal H, Selokar NL, Saini M, Singh MK, Chauhan MS, Palta P, Singla SK, Manik RS.||2018
Cell Reprogram 20(1):76-88.
|11.||m-carboxycinnamic acid bishydroxamide improves developmental competence, reduces apoptosis and alters epigenetic status and gene expression pattern in cloned buffalo (Bubalus bubalis) embryos.||Agarwal, H., Selokar, N.L., Saini, M., Singh, M.K., Chauhan, M.S., Palta, P., Singla, S.K. and Manik, R.S.||2018 Reproduction in Domestic Animals. Doi:10.1111/rda.13198.|
|12.||Establishment, growth, proliferation and gene expression of buffalo (Bubalus bubalis) transgenic fetal fibroblasts containing human insulin gene, and production of embryos by hand-made cloning using these cells.||Mehta, P., Kaushik, R., Singh, K.P., Sharma, A., Singh, M.K., Chauhan, M.S., Palta, P., Singla, S.K. ans Manik R.S. (2018).||2018
Cellular Reprogramming 20(2): 135-143.
|13.||Testicular cell-conditioned medium supports embryonic stem cell differentiation toward germ lineage and to spermatocyte- and oocyte-like cells.||Shah SM, Saini N, Singh MK, Manik RS, Singla SK, Palta P and Chauhan MS. 2016.||2016
Theriogenology, 86 (3): 715-729.
|14.||Effect of sex of embryo on developmental competence, epigenetic status, and gene expression in buffalo (Bubalus bubalis) embryos produced by hand-made cloning.||Sandhu A, Mohapatra SK, Agrawal H, Singh MK, Palta P, Singla SK, Chauhan MS, Manik RS.||2016
Cell Reprogram, 18(5):356-365.
|15.||Supplementation of tauroursodeoxycholic acid during IVC did not enhance in vitro development and quality of buffalo IVF embryos but combated endoplasmic reticulum stress.||A. Sharma, H. Agrawal, N. Mullani, A. Sandhu,M.K. Singh, M.S. Chauhan, S.K.Singla, P. Palta, R.S. Manik.||2015
Theriogenology 84 (2015) 200-207
|16.||Caspase-9 inhibitor Z-LEHD-FMK enhances the yield of in vitro produced buffalo (Bubalus bubalis) pre-implantation embryos and alters cellular stress response.||Mullani N, Singh MK, Sharma A, Rameshbabu K, Manik RS, Palta P, Singla SK and Chauhan MS.||2015
Research in Veterinary Science. 104:4-9.
|17.||Developmental competence of different quality bovine oocytes retrieved through ovum pick-up following in vitro maturation and fertilization.||Saini N, Singh MK, Shah SM, Singh KP, Kaushik R, Manik RS, Singla SK, Palta P and Chauhan MS.||2015
|18.||Molecular characterization and expression of buffalo (Bubalus bubalis) DEAD-box family VASA gene and mRNA transcript variants isolated from testis tissue.||Kaushik R, Singh KP, Bahuguna V, Rameshbabu K, Singh MK, Manik RS, Palta P, Singla SK and Chauhan MS. 2015.||2015
|19.||Cumulus cell-conditioned medium supports embryonic stem cell differentiation to germ cell-like cells.||Shah SM, Saini N, Ashraf S, Singh MK, Manik RS, Singla SK, Palta P and Chauhan MS. 2015.||2015
Reproduction Fertility and Development. 29: 679-693.
|20.||Comparative expression analysis of gametogenesis-associated genes in foetal and adult bubaline (Bubalus bubalis) ovaries and testes||Shah SM, Saini N, Ashraf S, Zandi M, Singh MK, Manik RS and Chauhan MS. 2015.||2015
Reproduction in Domestic Animals. 50(3), 365-377.
|21.||Optimization of Buffalo (Bubalus bubalis) Embryonic Stem Cell Culture System.||Zandi M, Muzaffar M, Shah SM, Singh MK, Palta P, Kumar Singla S, Manik R and Chauhan MS.||2015
Cell J. 17(2):264-73.
|22.||Activation and inhibition of the Wnt3A signaling pathway in buffalo (Bubalus bubalis) embryonic stem cells: effects of WNT3A, Bio and Dkk1.||Zandi M, Shah SM, Muzaffar M, Singh MK, Palta P, Singla SK, Manik RS and Chauhan MS.||2015
International Journal of Fertility and Sterility. 9(3): 361-70.
|23.||Developmental competence and expression pattern of bubaline (Bubalus bubalis) oocytes subjected to elevated temperatures during meiotic maturation in vitro.||Ashraf S, Shah SM, Saini N, Dhanda S, Kumar A, Goud TS, Singh MK, Chauhan MS and Upadhyay RC.||2014
Journal of assisted reproduction and genetics 31 (10), 1349-1360.
|24.||Quantitative expression of pluripotency-related genes in parthenogenetically produced buffalo (Bubalus bubalis) embryos and in putative embryonic stem cells derived from them.||Singh, K.P., Kaushik, R., Mohapatra, S.K., Garg, V., Rameshbabu, K., Singh, M.K., Palta, P., Manik, R.S., Singla, S.K. and Chauhan, M.S. 2014.||2014
Gene Expression Patterns 16(1), 23-30.
|25.||Construction of a recombinant human insulin expression vector for mammary gland-specific expression in buffalo (Bubalus bubalis) mammary epithelial cell line.||Kaushik, R., Singh, K.P., Kumari, A., Rameshbabu, K., Singh, M.K., Manik, R.S., Palta, P., Singla, S.K. and Chauhan, M.S. 2014.||2014
Molecular Biology Reports 41(9), 5891-5902.
|26.||Buffalo (Bubalus bubalis) ES cell-like cells are capable of in vitro skeletal myogenic differentiation.||M.K. Singh, K.P. Singh, D. Kumar, R.A. Shah, T. Anand, M.S. Chauhan, R.S. Manik, S.K. Singla and P. Palta. 2013.||Reprod. Dom. Anim., 48(2):284-91.|
|27.||WNT3A signaling pathway in buffalo (Bubalus bubalis) embryonic stem cells.||Zandi M, Muzaffar M, Shah SM, Kaushik R, Singh MK, Palta P, Singla SK, Manik RS and Chauhan MS. 2013.||2013
Reproduction, Fertility and Development: 26: 551-561.
|28.||Effect of physiologically relevant heat shock on development, apoptosis and expression of some genes in buffalo (Bubalus bubalis) embryos produced in vitro.||A. Yadav, K.P. Singh, M.K. Singh, N. Saini, P. Palta, R.S. Manik, S.K. Singla, R.C. Upadhyay and M.S. Chauhan. 2013.||2013
Reproduction in Domestic Animals, 48(5): 858-865.
|29.||Isolation, characterization, and EGFP expression in the buffalo (Bubalus bubalis) mammary gland epithelial cell line.||Kaushik R, Singh KP, Kumari A, Singh MK, Manik RS, Palta P, Singla SK and Chauhan MS.||2013
In Vitro Cellular & Dev Biol – Animal 49(1): 1-7.
|30.||Effects of GDNF, FGF2 and EGF on proliferation and expression of some important genes in buffalo (Bubalus bubalis) spermatogonial cells.||P.H. Kadam, S. Kala, H. Agrawal, K.P. Singh, M.K. Singh, M.S. Chauhan, P. Palta, S.K. Singla and R.S. Manik.||2012
Reproduction, Fertility and Development. 25: 1149-1157.
|31.||Equivalency of buffalo (Bubalus bubalis) embryonic stem cells derived from fertilized, parthenogenetic and handmade cloned embryos.||M. Muzaffar, N.L. Selokar, K.P. Singh, M. Zandi, M.K. Singh, R.A. Shah, M.S. Chauhan, S.K. Singla, P. Palta and R.S. Manik.||2012
Cellular Reprogramming, 14(3):267-79.
|32.||In vitro culture and morphological characterization of prepubertal buffalo (Bubalus bubalis) putative spermatogonial stem cell.||Kala S, Kaushik R, Singh KP, Kadam PH, Singh MK, Manik RS, Singla SK, Palta P, Chauhan MS.||2012
J. Assist. Reprod. Genet. 29(12): 1335-1342.
|33.||Expression pattern of pluripotent markers in different embryonic developmental stages of buffalo (Bubalus bubalis) embryos and putative embryonic stem cells generated by parthenogenetic activation.||Singh KP, Kaushik R, Garg V, Sharma R, George A, Singh MK, Manik RS, Palta P, Singla SK, Chauhan MS.||2012
Cell. Reprogram. 14(6): 530-538.
|34.||Oxygen concentration and cysteamine supplementation during in vitro production of buffalo (Bubalus bubalis) embryos affect mRNA expression of BCL-2, BCL-XL, MCL-1, BAX and BID.||Elamaran G, Singh KP, Singh MK, Singla SK, Chauhan MS, Manik RS, Palta P.||2012
Reprod. Domest. Anim. 47(6): 1027-1036.
|35.||Derivation of buffalo embryonic stem-like cells from in vitro-produced blastocysts on homologous and heterologous feeder cells.||Kumar, D., Anand, T., Singh, K.P., Singh, M.K., Shah, R.A., Chauhan, M.S. Palta, P., Singla, S.K., and Manik, R.S.||2011
J Assist Reprod Genet., 28: 697-688.
|36.||Buffalo (Bubalus bubalis) embryonic stem cell-like cells and preimplantation embryos exhibit comparable expression of pluripotency-related antigens.||T. Anand, D. Kumar, M.K. Singh, R.A Shah, M.S. Chauhan, R.S. Manik, S.K. Singla and P. Palta.||2011
Reprod. Dom. Anim. 6: 50-58.
|37.||Effect of sodium nitroprusside, a nitric oxide donor, and aminoguanidine, a nitric oxide synthase inhibitor, on in vitro development of buffalo (Bubalus bubalis) embryos.||S. Saugandhika, D. Kumar, M.K. Singh, R. Shah, T. Anand, M.S.Chauhan, R.S. Manik, S.K. Singla, P. Palta.||2010
Reprod. Dom. Anim., 45 (5): 931-933.
|38.||Pregnancies established from handmade cloned blastocysts reconstructed using skin fibroblasts in buffalo (Bubalus bubalis).||R.A. Shah, A. George, M.K. Singh, D. Kumar, T. Anand, M.S. Chauhan, R.S. Manik, P. Palta and S.K. Singla.||2009 Theriogenology. 71(8):1215-9.|
|39.||Hand-Made Cloned Buffalo (Bubalus bubalis) Embryos: Comparison of Different Media and Culture Systems.||R.A. Shah, A. George, M.K. Singh, D. Kumar, T. Anand, M.S. Chauhan, R.S. Manik, P. Palta and S.K. Singla.||2008
Cloning and Stem Cells. 10 (4):435-42. (Now Cellular Reprogramming)
|Articles in Indian Referred Journals (07)|
|40.||Comparative study of three different media to improve semen processing and flow cytometric analysis in bovine.||Nala N., Kumar V., Singh M.K., Manik R.S. and Singla S.K.
Journal of Animal Research, 7(3): 537-541.
|41.||Derivation, enrichment and characterization of goat (Capra hircus) spermatogonial stem cells from pre-pubertal testes.||Sharma A, Shah SM, Saini N, Kaushik R, Singh MK, Manik RS, Singla SK, Palta P and Chauhan MS.||2016
Indian J. Anim. Res., 50(5): 662-667.
|42.||‘Holi’: India’s first cattle calf produced through ovum pick-up – IVF technology- from an aged sahiwal cattle.||Saini N, Singh MK, Singh KP, Manik RS, Singla SK, Palta P and Chauhan MS.||2015
Indian J. Animal Sci. 85(3), 244-246.
|43.||Expression of apoptosis related genes in buffalo embryos produced through in vitro fertilization and parthenogenetic activation.||Singh, MK, Saw S, Singh KP, Saini N, Kaushik R, Manik RS, Palta P, Singla SK and Chauhan MS.||2014
Indian Journal of Animal Sciences84 (2): 146–151.
|44.||Characterization of the coding region of basic fibroblast growth factor (FGF2) c-DNA (open reading frame) in buffalo cumulus cells.||Kumar S, Singh MK, Kumar V, Palta P, Singla SK, Manik RS and Chauhan MS. (2013).||Indian Journal of Animal Sciences 83(7):744-746.|
|45.||Expression patterns of OCT-4 and NANOG genes in buffalo (Bubalus bubalis) embryos produced by in vitro fertilization or parthenogenetic activation.||Kumar D, Anand T, Shah RA, Singh MK, Chauhan MS and Manik RS.||2012
Ind. J. Anim. Sci. 82(8): 834-837.
|46.||Generation of buffalo embryonic stem cell-like cells from in vitro produced, day 8 hatched and day 9 expanded blastocysts||Kumar D, Anand T, Singh KP, Shah RA, Singh MK, Chauhan MS, Palta P, Singla SK and Manik RS.||2012
Ind. J. Anim. Sci. 82(8): 838-843.
Present Lab Members: Dr. M.K. Singh & Dr. P. Palta
Students currently enrolled
|Sr. No.||Name of student||Class||Dissertation topic:|
|1.||Mr. Gaurav Tripathi
|Ph.D. Scholar||Effect of miRNA- 29b and miRNA- 449b on buffalo transgenic embryos, containing human insulin gene, produced by handmade cloning.|
|2.||Ms. Tanya Gupta
|Ph.D. Scholar||Global DNA methylation profile of spermatozoa of cloned buffalo bulls and their respective donor bulls.|
|3.||Ms. Kumari Rinka
|Ph.D. Scholar||Effect of trichostatin A and 5-aza-2’-deoxycytidine on the developmental competence and transcriptome profile of buffalo embryos produced by Hand-made cloning”|
|4.||Ms. Sonal Gupta
|Ph.D. Scholar||Effect of oxamflatin and ascorbic acid on the developmental competence and transcriptome profile of buffalo embryos produced by Hand- made cloning (Proposed).|
|5.||Mr. Atul Kaushal
|Ph.D. Scholar||Course work|
|6.||Mr. Prem Kumar
|Ph.D. Scholar||Course work|
|7.||Mr. Faiz Akram
|M.Sc. Scholar||Course work|
|8.||Ms. Smriti Gupta
|M.Sc. Scholar||Course work|
|9.||Ms. Kanika Gandhi
|M.Sc. Scholar||Course work|
|M.Sc. Scholar||Course work|
Technologies Developed (if any):
- Ovum pick-up-IVF technique standardised in indigenous cattle (India’s first cattle calf “Holi” produced)
- Hand-guided Cloning protocol in buffalo developed.
- Established the procedure of buffalo embryonic stem cell culture and their induced differentiation into myogenic cells.
- Establishment of spermatogonial stem cell line in buffalo and goat.
- Protocol established for transgenic embryo production in goat and buffalo.